1996
DOI: 10.1182/blood.v88.4.1284.bloodjournal8841284
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Characterization of a bipotent erythro-megakaryocytic progenitor in human bone marrow

Abstract: The aim of the present study was to determine if the human erythroid (E) and megakaryocytic (MK) lineages were closely linked to the existence of a bipotent burst-forming unit (BFU) E/MK progenitor. In methylcellulose cultures, BFU-E/MK colonies were observed at day 12 and closely resembled mature BFU-E with the exception that the erythroid component was surrounded by MK. These colonies were quite different from the colony forming unit (CFU)-GEMM-derived colonies, which were composed of a larger number of eryt… Show more

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Cited by 236 publications
(101 citation statements)
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“…Interestingly, the increase in megakaryocyte size and ploidy during the first year of age parallels the switching of haemoglobin from fetal to adult forms. It may be possible that shared transcriptional regulatory mechanisms are operating in megakaryocytes and erythrocytes during postnatal development, given the close relationship between both lineages (Romeo et al, 1990;Shivdasani et al, 1995;Debili et al, 1996;Kieran et al, 1996;Tsang et al, 1998;Vyas et al, 1999).…”
Section: Discussionmentioning
confidence: 99%
“…Interestingly, the increase in megakaryocyte size and ploidy during the first year of age parallels the switching of haemoglobin from fetal to adult forms. It may be possible that shared transcriptional regulatory mechanisms are operating in megakaryocytes and erythrocytes during postnatal development, given the close relationship between both lineages (Romeo et al, 1990;Shivdasani et al, 1995;Debili et al, 1996;Kieran et al, 1996;Tsang et al, 1998;Vyas et al, 1999).…”
Section: Discussionmentioning
confidence: 99%
“…The most interesting functions of lineage-restricted transcription factors are exercised at the branch points of cellular hierarchies, where cell-specific programs of gene expression are activated or reinforced to permit emergence of a lineage identity. While there is increasing evidence that MKs derive from a bipotential erythro-MK progenitor [101], there are presently few clues about the transcriptional basis for defining the MK lineage. Perhaps most frustrating is the repeated observation that important aspects of differentiation of both erythroid cells and MKs depend, to varying degrees, on the same handful of hematopoietic transcription factors: GATA proteins, FOG-1, and NF-E2.…”
Section: Transcriptional Control Of Cell Fatementioning
confidence: 99%
“…Erythrocytes and megakaryocytes have been postulated to derive from a common bi-potent progenitor cell (McDonald & Sullivan, 1993). Originally supported by indirect evidence, such as the simultaneous expression of erythroid (glycophorin A) and megakaryocyte (glycoprotein IIb/IIIa) lineage markers on primitive haemopoietic cells (Rowley et al, 1992), more direct proof of the existence of a common progenitor has recently been provided by cell cloning studies (Debili et al, 1996). Given the closeness of their origins, it may not be surprising that two of the major regulators of erythroid and megakaryocyte development, EPO and TPO, have been shown to have a high degree of amino terminal amino acid sequence homology (Lok et al, 1994).…”
mentioning
confidence: 99%