Characterization of a cDNA encoding Arabidopsis secretory phospholipase A2-α, an enzyme that generates bioactive lysophospholipids and free fatty acids

Ryu, Stephen B.; Lee, Hyoung Yool; Doelling, Jed H.; Palta, Jiwan P.

doi:10.1016/j.bbalip.2005.08.005

Cited by 23 publications

(29 citation statements)

References 41 publications

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“…The amino acid sequence, enzymatic properties, and expression pattern of AtsPLA 2 -α are distinguishable from those of the other three AtsPLA 2 isoforms, suggesting that it may play a distinct role that differs from that of the other AtsPLA 2 s (24). Here, we show that the Arabidopsis TF AtMYB30 partially modifies the targeting of AtsPLA 2 -α from cytoplasmic vesicles to the nucleus, where both proteins interact.…”

Section: Discussionmentioning

confidence: 76%

At sPLA ₂ -α nuclear relocalization by the Arabidopsis transcription factor AtMYB30 leads to repression of the plant defense response

Froidure

Canonne

Daniel

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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We hereby confirm that the original images are those first published in PNAS. When preparing the figures for submission to PNAS, the raw images were cropped and/or stretched to match the other blots and saved in the format for submission. Unfortunately, we did not systematically archive an independent copy of each raw image, and only the final version of the figures was stored. Fig. 3 appears to have areas of unmarked splicing and background inconsistencies, but we are confident, however, in the scientific accuracy of the data despite being unable to provide the original images."Further, we acknowledge that images of yeast colonies in Fig. S1B are indeed duplicated. Our intention was to represent the presence (or absence) of yeast growth observed with the different protein combinations. We recognize that this should have been clearly indicated in the figure legend and apologize for this omission. We have been able to retrieve the original results and prepared the revised figure below showing growth of yeast colonies expressing the different protein combinations. We apologize for any inconvenience the publication of these figures may have caused." The corrected Fig. S1 and its corrected legend appear below. The SI has been corrected online.

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Section: Discussionmentioning

confidence: 76%

At sPLA ₂ -α nuclear relocalization by the Arabidopsis transcription factor AtMYB30 leads to repression of the plant defense response

Froidure

Canonne

Daniel

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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“…Among the top selected genes, the lipid degradation pathway gene BrPLA2-ALPHA (Bra038125) had the highest degree of connection and a cis-eQTL on A05, colocating with major PUFA faQTLs for linoleic acid (C18:2) and eicosadienoic acid (C20:2) and minor faQTLs for other FAs (Table II). Ryu et al (2005) functionally characterized the BrPLA2-ALPHA gene and concluded that it has an acyl preference for linoleic acid over palmitic acid in phospholipid hydrolysis in Arabidopsis. They also reported a role of this gene in the release of free FAs and lysophospholipids from membrane phospholipids.…”

Section: Discussionmentioning

confidence: 99%

A Systems Genetics Approach Identifies Gene Regulatory Networks Associated with Fatty Acid Composition in Brassica rapa Seed

et al. 2015

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Fatty acids in seeds affect seed germination and seedling vigor, and fatty acid composition determines the quality of seed oil. In this study, quantitative trait locus (QTL) mapping of fatty acid and transcript abundance was integrated with gene network analysis to unravel the genetic regulation of seed fatty acid composition in a Brassica rapa doubled haploid population from a cross between a yellow sarson oil type and a black-seeded pak choi. The distribution of major QTLs for fatty acids showed a relationship with the fatty acid types: linkage group A03 for monounsaturated fatty acids, A04 for saturated fatty acids, and A05 for polyunsaturated fatty acids. Using a genetical genomics approach, expression quantitative trait locus (eQTL) hotspots were found at major fatty acid QTLs on linkage groups A03, A04, A05, and A09. An eQTL-guided gene coexpression network of lipid metabolism-related genes showed major hubs at the genes BrPLA2-ALPHA, BrWD-40, a number of seed storage protein genes, and the transcription factor BrMD-2, suggesting essential roles for these genes in lipid metabolism. Three subnetworks were extracted for the economically important and most abundant fatty acids erucic, oleic, linoleic, and linolenic acids. Network analysis, combined with comparison of the genome positions of cis-or trans-eQTLs with fatty acid QTLs, allowed the identification of candidate genes for genetic regulation of these fatty acids. The generated insights in the genetic architecture of fatty acid composition and the underlying complex gene regulatory networks in B. rapa seeds are discussed.

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“…4; Supplemental Table S3-2). PLA2a, a secretory phospholipase A2 enzyme, specifically hydrolyzes the sn-2 position of phospholipids and has a preference toward the linoleoyl acyl chain (Ryu, 2004;Ryu et al, 2005). ROD1 encodes a phosphatidylcholine:diacylglycerol cholinephosphotransferase that is involved in a major reaction for the transfer of C18:1 to phosphatidylcholine for desaturation as well as for the reverse transfer of C18:2 and C18:3 to the TAG synthesis pathway ( Fig.…”

Section: Discussionmentioning

confidence: 99%

MYB89 Transcription Factor Represses Seed Oil Accumulation

et al. 2016

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ORCID IDs: 0000-0002-0738-7899 (M.Z.); 0000-0002-2121-9517 (M.C.).In many higher plants, seed oil accumulation is precisely controlled by intricate multilevel regulatory networks, among which transcriptional regulation mainly influences oil biosynthesis. In Arabidopsis (Arabidopsis thaliana), the master positive transcription factors, WRINKLED1 (WRI1) and LEAFY COTYLEDON1-LIKE (L1L), are important for seed oil accumulation. We found that an R2R3-MYB transcription factor, MYB89, was expressed predominantly in developing seeds during maturation. Oil and major fatty acid biosynthesis in seeds was significantly promoted by myb89-1 mutation and MYB89 knockdown; thus, MYB89 was an important repressor during seed oil accumulation. RNA sequencing revealed remarkable up-regulation of numerous genes involved in seed oil accumulation in myb89 seeds at 12 d after pollination. Posttranslational activation of a MYB89-glucocorticoid receptor fusion protein and chromatin immunoprecipitation assays demonstrated that MYB89 inhibited seed oil accumulation by directly repressing WRI1 and five key genes and by indirectly suppressing L1L and 11 key genes involved in oil biosynthesis during seed maturation. These results help us to understand the novel function of MYB89 and provide new insights into the regulatory network of transcriptional factors controlling seed oil accumulation in Arabidopsis.

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Characterization of a cDNA encoding Arabidopsis secretory phospholipase A2-α, an enzyme that generates bioactive lysophospholipids and free fatty acids

Cited by 23 publications

References 41 publications

At sPLA ₂ -α nuclear relocalization by the Arabidopsis transcription factor AtMYB30 leads to repression of the plant defense response

At sPLA ₂ -α nuclear relocalization by the Arabidopsis transcription factor AtMYB30 leads to repression of the plant defense response

A Systems Genetics Approach Identifies Gene Regulatory Networks Associated with Fatty Acid Composition in Brassica rapa Seed

MYB89 Transcription Factor Represses Seed Oil Accumulation

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Characterization of a cDNA encoding Arabidopsis secretory phospholipase A2-α, an enzyme that generates bioactive lysophospholipids and free fatty acids

Cited by 23 publications

References 41 publications

At sPLA 2 -α nuclear relocalization by the Arabidopsis transcription factor AtMYB30 leads to repression of the plant defense response

At sPLA 2 -α nuclear relocalization by the Arabidopsis transcription factor AtMYB30 leads to repression of the plant defense response

A Systems Genetics Approach Identifies Gene Regulatory Networks Associated with Fatty Acid Composition in Brassica rapa Seed

MYB89 Transcription Factor Represses Seed Oil Accumulation

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At sPLA ₂ -α nuclear relocalization by the Arabidopsis transcription factor AtMYB30 leads to repression of the plant defense response

At sPLA ₂ -α nuclear relocalization by the Arabidopsis transcription factor AtMYB30 leads to repression of the plant defense response