Characterization of a chemosensory protein (ASP3c) from honeybee (<i>Apis mellifera</i> L.) as a brood pheromone carrier

Briand, Loı̈c; Swasdipan, Nicharat; Nespoulous, Claude; Bézirard, Valérie; Blon, Florence; Huet, Jean‐Claude; Ebert, Paul R.; Pernollet, Jean‐Claude

doi:10.1046/j.1432-1033.2002.03156.x

Cited by 135 publications

(115 citation statements)

References 62 publications

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“…Obp13, 14, 15, and chemosensory protein 3 (ASP3) were identified in the hemolymph of A. mellifera summer workers (Bogaerts et al 2009), and Obp14 was found in winter worker hemolymph (Erban et al 2013). Briand et al (2002) observed that CSP3 is a brood pheromone carrier in A. mellifera . Foret et al (2007) found CSP3 in late larvae, but no detectable expression of CSP3 was found during the initial stages of pupal development (24 h); however, the CSP3 transcripts were, again, detectable in red-eye pupae, and the level gradually increased as the head became dark.…”

Section: Olfaction-odorant-binding and Chemosensory Proteinsmentioning

confidence: 93%

Detailed proteome mapping of newly emerged honeybee worker hemolymph and comparison with the red-eye pupal stage

et al. 2016

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-The honeybee, Apis mellifera , undergoes complete metamorphosis before transitioning to the adult stage. The newly emerged individual and the red-eye pupa stage are well defined and easily recognizable in the time life cycle honeybee and, therefore, very useful for studying physiological and developmental factors. We analyzed in detail the hemolymph proteome of newly emerged honeybee worker using 2D-E-MS/MS (pI 3-10 and 4-7). The comparison of identical hemolymph volumes (20 μL per 2D-E) for newly emerged bee and red-eye pupa revealed a dramatic decrease in the number of spots (qualitative changes) and overall protein quantity during the non-feeding stage. The results increase our knowledge about honeybee metamorphosis during the non-feeding period and clarify previous findings regarding particular proteins. The results will be useful for future comparative physiological, developmental, and host-pathogen studies on individual or population level.Apis mellifera / hemolymph / metamorphosis / hexamerin / ferritin

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Section: Olfaction-odorant-binding and Chemosensory Proteinsmentioning

confidence: 93%

Detailed proteome mapping of newly emerged honeybee worker hemolymph and comparison with the red-eye pupal stage

et al. 2016

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“…Although a few studies have sought to implicate various small soluble proteins in pheromone recognition (17,(20)(21)(22)(23), it seems more likely that these proteins are involved in generic odorant solubilization and play little role in odor coding (24,25). The only known hymenopteran pheromone ligand-receptor pair to date is the honey bee (Apis mellifera) queen pheromone 9-ODA and the OR AmelOR11 (26).…”

Section: Significancementioning

confidence: 99%

Transcriptomics and neuroanatomy of the clonal raider ant implicate an expanded clade of odorant receptors in chemical communication

McKenzie

Fetter-Pruneda

Ruta

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

123

182

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A major aim of sociogenomic research is to uncover common principles in the molecular evolution of sociality. This endeavor has been hampered by the small number of specific genes currently known to function in social behavior. Here we provide several lines of evidence suggesting that ants have evolved a large and novel clade of odorant receptor (OR) genes to perceive hydrocarbonbased pheromones, arguably the most important signals in ant communication. This genomic expansion is also mirrored in the ant brain via a corresponding expansion of a specific cluster of glomeruli in the antennal lobe. We show that in the clonal raider ant, hydrocarbon-sensitive basiconic sensilla are found only on the ventral surface of the female antennal club. Correspondingly, nearly all genes in a clade of 180 ORs within the 9-exon subfamily of ORs are expressed exclusively in females and are highly enriched in expression in the ventral half of the antennal club. Furthermore, we found that across species and sexes, the number of 9-exon ORs expressed in antennae is tightly correlated with the number of glomeruli in the antennal lobe region innervated by odorant receptor neurons from basiconic sensilla. Evolutionary analyses show that this clade underwent a striking gene expansion in the ancestors of all ants and slower but continued expansion in extant ant lineages. This evidence suggests that ants have evolved a large clade of genes to support pheromone perception and that gene duplications have played an important role in the molecular evolution of ant communication.sociogenomics | chemosensation | social evolution | antennal lobe | Formicidae T he field of sociogenomics seeks to understand the molecular basis of sociality, asking how social life evolved and how it is governed at the genomic level (1). A particularly pertinent question in the field is the role of novel genes vs. conserved genes in the evolution and regulation of social behaviors, including communication (2, 3). Communication is important to a broad range of organisms, yet relatively little is known about the genetic components of communication systems and how they evolve. A particularly interesting question in the field is how social signals are perceived and what sort of genetic and physiological specializations facilitate signal perception in highly social organisms (4, 5). In the most tractable sociogenomic models, the eusocial hymenopteran insects, communication is largely chemical, and the genetics and physiology of chemosensation in these species may hold the key to understanding their advanced communication (4,6, 74).Much is already known about the insect chemosensory system in general (8,9). Briefly, chemicals are detected by porous sensory hairs (sensilla) located on chemosensory organs such as the legs, wings, palps, and especially the antennae. The sensilla house the dendrites of olfactory and/or gustatory receptor neurons (ORNs/GRNs) with receptor proteins in the olfactory receptor (OR), the gustatory receptor (GR), or the ionotropic receptor (IR) gene ...

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“…Furthermore, several haemolymph proteins were resolved by twodimensional gel electrophoresis that were not correlated with microbial challenge, among them apolipophorin-III (ApoLp-III), another component of the lipid transport system (Weers and Ryan, 2006), antennal-specific protein 3c (ASP3c), a soluble chemosensory protein (Briand et al, 2002;Forêt et al, 2007), and three members of the odorant binding-like protein (OBP) family, OBP 13, OBP 14 and OBP 18. Of the three OBPs identified in the haemolymph of adult drones, OBP 14 was present also in drone larvae (Fig.2) whereas OBP 13 and OBP18 were not detected in larval haemolymph (data not shown).…”

Section: Adult Young Drones Respond To Septic Injury With a Broad Arrmentioning

confidence: 99%

Honey bee drones maintain humoral immune competence throughout all life stages in the absence of vitellogenin production

Gätschenberger

Gimple

Tautz

et al. 2012

Journal of Experimental Biology

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SUMMARYDrones are haploid male individuals whose major social function in honey bee colonies is to produce sperm and mate with a queen. In spite of their limited tasks, the vitality of drones is of utmost importance for the next generation. The immune competence of drones -as compared to worker bees -is largely unexplored. Hence, we studied humoral and cellular immune reactions of in vitro reared drone larvae and adult drones of different age upon artificial bacterial infection. Haemolymph samples were collected after aseptic and septic injury and subsequently employed for (1) the identification of immune-responsive peptides and/or proteins by qualitative proteomic analyses in combination with mass spectrometry and (2) the detection of antimicrobial activity by inhibition-zone assays. Drone larvae and adult drones responded with a strong humoral immune reaction upon bacterial challenge, as validated by the expression of small antimicrobial peptides. Young adult drones exhibited a broader spectrum of defence reactions than drone larvae. Distinct polypeptides including peptidoglycan recognition protein-S2 and lysozyme 2 were upregulated in immunized adult drones. Moreover, a pronounced nodulation reaction was observed in young drones upon bacterial challenge. Prophenoloxidase zymogen is present at an almost constant level in non-infected adult drones throughout the entire lifespan. All observed immune reactions in drones were expressed in the absence of significant amounts of vitellogenin. We conclude that drones -like worker bees -have the potential to activate multiple elements of the innate immune response. Supplementary material available online at

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Characterization of a chemosensory protein (ASP3c) from honeybee (Apis mellifera L.) as a brood pheromone carrier

Cited by 135 publications

References 62 publications

Detailed proteome mapping of newly emerged honeybee worker hemolymph and comparison with the red-eye pupal stage

Detailed proteome mapping of newly emerged honeybee worker hemolymph and comparison with the red-eye pupal stage

Transcriptomics and neuroanatomy of the clonal raider ant implicate an expanded clade of odorant receptors in chemical communication

Honey bee drones maintain humoral immune competence throughout all life stages in the absence of vitellogenin production

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