Characterization of a compartment syndrome–like injury model

Oyster, Nick; Witt, Michelle R.; Gharaibeh, Burhan; Poddar, Minakshi; Schneppendahl, Johannes; Huard, Johnny

doi:10.1002/mus.24461

Cited by 20 publications

(19 citation statements)

References 39 publications

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“…Tissue edema and disorganization were early observed 24 h after injury, and within the first 4 days, 50% of the muscle fibres underwent degeneration. Immune cell infiltration, as described in other models, occurred within 2 days after damage and persisted throughout the first week, with a peak at day four [154][155][156]. Following the acute inflammatory response, fibroblast and myofibroblast growth resulted in enhanced collagen deposition, also supporting the formation of newly regenerating fibers.…”

Section: Crush Injury and Ischemia-reperfusion Damagesupporting

confidence: 54%

“…Indeed, although early markers of satellite cell activation such as Pax7 and MyoD were observed early after damage (2 days), regenerating myofibers were detected 7 days after injury in correspondence with the phase of collagen deposition (Figure 1) [154]. The extensive damage induced by muscle compression was also highlighted by signs of denervation, such as the dispersed localization of acetylcholine receptors around crushed myofibers and of vasculature alterations, including neo-angiogenesis preceded by the presence of enlarged vessels and hemorrhagic areas [154,156,157].…”

Section: Crush Injury and Ischemia-reperfusion Damagementioning

confidence: 98%

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Mechanisms Regulating Muscle Regeneration: Insights into the Interrelated and Time-Dependent Phases of Tissue Healing

Forcina

Cosentino

Musarò

2020

Cells

162

130

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Despite a massive body of knowledge which has been produced related to the mechanisms guiding muscle regeneration, great interest still moves the scientific community toward the study of different aspects of skeletal muscle homeostasis, plasticity, and regeneration. Indeed, the lack of effective therapies for several physiopathologic conditions suggests that a comprehensive knowledge of the different aspects of cellular behavior and molecular pathways, regulating each regenerative stage, has to be still devised. Hence, it is important to perform even more focused studies, taking the advantage of robust markers, reliable techniques, and reproducible protocols. Here, we provide an overview about the general aspects of muscle regeneration and discuss the different approaches to study the interrelated and time-dependent phases of muscle healing.

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Section: Crush Injury and Ischemia-reperfusion Damagesupporting

confidence: 54%

Section: Crush Injury and Ischemia-reperfusion Damagementioning

confidence: 98%

Mechanisms Regulating Muscle Regeneration: Insights into the Interrelated and Time-Dependent Phases of Tissue Healing

Forcina

Cosentino

Musarò

2020

Cells

162

130

View full text Add to dashboard Cite

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“…Experimental evidence suggests that in ammation is one of the main driving factors of secondary injury following ACS [32][33][34]. Recent studies have shown that transplanted MSCs reduce IRI in skeletal muscle by inhibiting the in ammatory response and oxidative stress [35,36] while promoting muscle tissue repair after injury [37].…”

Section: Discussionmentioning

confidence: 99%

Embryonic Stem Cell-derived Mesenchymal Stem Cells Alleviate Skeletal Muscle Injury Induced by Acute Compartment Syndrome

Jiang

Yang

Liu

et al. 2022

Preprint

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Background Acute compartment syndrome (ACS), a well-known complication of musculoskeletal injury, results in muscle necrosis and cell death. Embryonic stem cell-derived mesenchymal stem cells (ES-MSCs) have been shown to be a promising therapy for ACS. However, their effectiveness and potentially protective mechanism remain unknown. The present study was designed to investigate the efficacy and underlying mechanism of ES-MSCs in ACS-induced skeletal muscle injury. Method A total of 120 male Sprague–Dawley (SD) rats underwent 2 hours of intracompartmental pressure elevation by saline infusion into the anterior compartment of the left hindlimb to establish the ACS model. ES-MSCs were differentiated from the human embryonic stem cell (ES) line H9. A dose of 1.2×106 of ES-MSCs was intravenously injected during fasciotomy. Post-ACS assessments included skeletal edema index, serum indicators, histological analysis, apoptosis, fibrosis, regeneration, and functional recovery of skeletal muscle. Then, fluorescence microscopy was used to observe the distribution of labeled ES-MSCs in vivo, and western blotting and immunofluorescence analyses were performed to examine M2 macrophage infiltration in skeletal muscle. Finally, we used liposomal clodronate to deplete macrophages and reassess skeletal muscle injury in response to ES-MSC therapy. Result ES-MSCs significantly reduced systemic inflammatory responses, ACS-induced skeletal muscle edema and cell apoptosis. In addition, ES-MSCs inhibited skeletal muscle fibrosis and increased regeneration and functional recovery of skeletal muscle after ACS. The beneficial effects of ES-MSCs on ACS-induced skeletal muscle injury were accompanied by an increase in CD206-positive M2 macrophage polarization. After depleting macrophages with liposomal clodronate, the beneficial effects of ES-MSCs were abolished. Conclusion Our findings suggest that embryonic stem cell-derived mesenchymal stem cells infusion could effectively alleviate ACS-induced skeletal muscle injury, in which the protective effects were related to M2 macrophage polarization.

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“…Fortunately a rare occurrence, a phlebotomy tourniquet left in place for prolonged periods of time (e.g., hours) can result in the development of extremity compartment syndrome-a potentially limb-threatening condition [106,107]. Compartment syndrome is a serious injury defined by an increase in pressure within a fascia-enclosed muscle compartment that results in compromised circulation leading to nerve damage and muscle necrosis [108]. This can lead to permanent disability, amputation, or even death from the release of toxic metabolites.…”

Section: Special Topicsmentioning

confidence: 99%

Dangers of Peripheral Intravenous Catheterization: The Forgotten Tourniquet and Other Patient Safety Considerations

Kaur¹,

Rickard²,

Domer³

et al. 2019

Vignettes in Patient Safety - Volume 4 [Working Title]

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Intravenous catheterization is a widely used invasive procedure, with applications in both ambulatory and hospital settings. Due to its inherently invasive nature, intravenous (IV) therapy is associated with a number of potential complications, many of which are directly relevant to patient safety (PS). PIV-related morbidity may be due to mechanical or nonmechanical factors. The most frequent nonmechanical peripheral venous catheterization adverse events (PVCAEs) include insertion site pain, phlebitis, hematoma formation, and infusate extravasation. The most common mechanical PVCAE is catheter obstruction/occlusion and dislodgement. Significant complications can also occur with the administration of incorrect type or wrong amount of IV fluids. Moreover, simultaneous infusion of incompatible medications can result in infusate precipitation. Finally, less frequent but significant complications have been reported, including bloodstream and local infections, air embolization, nerve damage, arterial puncture, skin necrosis associated with vasopressor infusions, and limb-threatening forgotten tourniquet events. Taken together, the above complications can lead to substantial patient discomfort, unnecessary or prolonged hospitalization, increased costs, and additional downstream morbidity. Efforts to prevent PVCAEs and improve patient outcomes should involve thorough provider education, clinical vigilance by all involved healthcare providers, health service level strategies, as well as the proactive participation of all stakeholders, including patients and their families.

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Characterization of a compartment syndrome–like injury model

Cited by 20 publications

References 39 publications

Mechanisms Regulating Muscle Regeneration: Insights into the Interrelated and Time-Dependent Phases of Tissue Healing

Mechanisms Regulating Muscle Regeneration: Insights into the Interrelated and Time-Dependent Phases of Tissue Healing

Embryonic Stem Cell-derived Mesenchymal Stem Cells Alleviate Skeletal Muscle Injury Induced by Acute Compartment Syndrome

Dangers of Peripheral Intravenous Catheterization: The Forgotten Tourniquet and Other Patient Safety Considerations

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