Characterization of a coumarin C-/O-prenyltransferase and a quinolone C-prenyltransferase from Murraya exotica

Abstract

Prenyltransferases (PTs) play important roles in the biosynthesis and structural diversification of natural products. In the present study, two new PTs were characterized from a medicinal plant Murraya exotica. MePT1...

Substrate-Dependent Alteration in the <i>C</i>- and <i>O</i>-Prenylation Specificities of <i>Cannabis</i> Prenyltransferase

“…10,11) As the only PT that catalyzes both C-and O-prenylations, MePT1 from Murraya exotica synthesizes a trace amount of 7-O-geranylumbelliferone along with C-geranylated major products. 12) In addition, plant aromatic PTs are characterized by their high substrate specificity, and in general recognize aromatic substrates with specific skeletons. 9) Therefore, CsPT4 is a notable enzyme that can accept both resorcylic acid and acylphloroglucinol substrates and alters the C-/O-prenylation patterns depending on the substrate skeleton.…”

Identification of O-Methyltransferases Potentially Contributing to the Structural Diversity of 2-(2-Phenylethyl)chromones in Agarwood

“…Relative gene transcript levels were calculated using the 2 −ΔΔCt method and normalized to the AsRPL gene as an internal control. 29,30 Statistical Analysis. Data from three independent experiments were analyzed by using GraphPad Prism 7.0 software.…”

“…A thermal cycler was carried out as the following program: one cycle of 95 °C for 3 min and 35 cycles of 95 °C for 30 s and 60 °C for 30 s. Three biological replicates were analyzed for each material. Relative gene transcript levels were calculated using the 2 –ΔΔ Ct method and normalized to the AsRPL gene as an internal control. , …”

Terpenoids from Penicillium chrysogenum MT-40, an endophytic fungus isolated from Huperzia serrata

“…The EtOAc fraction (130 g) was subjected to silica gel column chromatography with a stepwise gradient elution using dichloromethane, dichloromethane-MeOH, and MeOH, to yield fourteen fractions (Fr. [1][2][3][4][5][6][7][8][9][10][11][12][13][14]. Fr.7 (1.25 g) was subjected to an RP-C 18 open column (MeOH/H 2 O, 35 : 65-100 : 0 gradient) to provide 13 fractions (Fr.7.1-7.13).…”

“…Terpenoids biosynthetically derived from the universal C5 units of dimethylallyl diphosphate (DMAPP) and isopentenyl diphosphate (IPP) are the largest family of natural products with an astonishing diversity of structures (>80 000) and biological activities. 1,2 For example, the well-known strigolactones and gibberellin are important signaling molecules involved in plant seed germination and growth, [3][4][5] ocimene, 6 menthol, 7 and santalenes 8 are essential aromatic ingredients in perfumes, and artemisinin 9 and taxol 10 are used as front-line therapeutics to treat malaria and cancer.…”