Characterization of a Gene Encoding 3-Deoxy-D-Arabino-Heptulosonate 7-Phosphate Synthase from Rice

Abstract

D-arabino-heptulosonate 7-phosphate synthase (DAHPS) is an entry enzyme of the shikimate pathway which is responsible for primary carbohydrate metabolism with the biosynthesis of most aromatic amino acids in microorganisms and plants. Enzymes of the pathway are drawing attention in the recent years because they are recognized as potential targets for design of antimicrobial drugs and herbicides. Analysis of sequences from rice revealed that the OsDAHPS showed a full-length open reading frame consisting of 537 amino acids, which encoded for a protein of approximately 59.0 kDa. The predicted amino acid sequence of OsDAHPS is highly homologous to those of DAHPS enzymes from many plants. The OsDAHPS expression in a aroH mutant of Escherichia coli showed that the gene was functionally capable of complementing the mutant. These results showed that the OsDAHPS encoded for a protein in 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase in rice.

BIOMASS YIELD 1 regulates sorghum biomass and grain yield via the shikimate pathway

“…Much is known about the function of DAHPS in various microorganisms ( Maeda and Dudareva, 2012 ); however, it shares less than 20% sequence homology between microorganisms and plants and has obvious differences in amino acid composition and properties, suggesting that the functions may differ ( Arcuri et al , 2004 ; Chávez-Béjar, 2008 ). To date, DAHPS proteins have been identified in several plant species including tobacco ( Wang et al , 1991 ), Arabidopsis ( Keith et al , 1991 ), potato ( Zhao and Herrmann, 1992 ), Populus trichocarpa ( Tuskan et al , 2006 ), grapevine ( Zhang et al , 2011 ), tomato ( Tzin et al , 2012 ), and rice ( Pagor et al , 2015 ), but the specific functions and regulatory mechanisms of DAHPS in plants are not well understood.…”