Characterization of a Gene Identified in Pathotype 5 of the Clubroot Pathogen<i>Plasmodiophora brassicae</i>

Zhang, H; Feng, Jie; Vp, Manolii; Se, Strelkov; Sf, Hwang

doi:10.1094/phyto-10-14-0270-r

Cited by 22 publications

(6 citation statements)

References 41 publications

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“…Even many strategies have been proposed for clubroot disease control, but the use of resistant cultivars is still believed to be most effective (Peng et al, 2014;Zhang et al, 2015). The experiment result here may lay a foundation to further research about P. brassicae.…”

Section: Discussionmentioning

confidence: 65%

Pathogen Identification of Clubroot Disease in Chinese Cabbage from Yuanyang County, Henan Province

Li¹,

Yuan²,

Zhao³

et al. 2017

mpb

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To clarify the causal agent of clubroot disease in Chinese cabbage from Yuanyang experimental base, the isolate YY was collected from the clubroot for further research. After morphological, cytological observation and pathogenicity identification, the pathogen was preliminary inferred as Plasmodiophora brassicae. In order to quickly detect the pathogen, specific primers were designed according to D85819 and Pro1 gene, respectively, and used to amplify the expected fragment from the template DNA of target pathogen. After PCR and sequence analysis, they showed 99% and 93% identity with the D85819 and Pro1 gene sequences of P. brassicae from NCBI respectively. These results indicated that the pathogen was finally confirmed as P. brassicae, which were coincident with preliminary speculation. Moreover, Pro1-specific marker developed in this study can be applied to quick and accurate detection of P. brassicae isolate, which appears a useful assay method for detecting P. brassicae resting spores and improves efforts to halt the dissemination and better control this pathogen.

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Section: Discussionmentioning

confidence: 65%

Pathogen Identification of Clubroot Disease in Chinese Cabbage from Yuanyang County, Henan Province

Li¹,

Yuan²,

Zhao³

et al. 2017

mpb

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“…The results of this study indicated that P. brassicae completed the primary infection stage in the two resistant B. rapa hosts, and that the secondary zoospores produced following primary infection invaded the cortex. Similarly, Zhang et al (2015) reported that secondary infection occurred in clubroot susceptible and resistant hybrid canola cultivars by single-spore isolate. This may reflect the main pathotype from the field isolate invade the cortex of clubroot-resistant hosts.…”

Section: Discussionmentioning

confidence: 91%

Histopathology of the Plasmodiophora brassicae-Chinese Cabbage Interaction in Hosts Carrying Different Sources of Resistance

Liu

Strelkov²,

Sun³

et al. 2022

Front. Plant Sci.

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Clubroot is a serious soil-borne disease of crucifers caused by the obligate parasite Plasmodiophora brassicae. The genetic basis and histopathology of clubroot resistance in two Chinese cabbage (Brassica rapa ssp. pekinensis) inbred lines Bap055 and Bap246, challenged with pathotype 4 of P. brassicae, was evaluated. The Chinese cabbage cultivar “Juxin” served as a susceptible check. The resistance in Bap055 was found to be controlled by the CRa gene, while resistance in Bap246 fit a model of control by unknown recessive gene. Infection of the roots by P. brassicae was examined by inverted microscopy. Despite their resistance, primary and secondary infection were observed to occur in Bap055 and Bap246. Primary infection was detected at 2 days post-inoculation (DPI) in “Juxin,” at 4 DPI in Bap055, and at 6 DPI in Bap246. Infection occurred most quickly on “Juxin,” with 60% of the root hairs infected at 10 DPI, followed by Bap055 (31% of the root hairs infected at 12 DPI) and Bap246 (20% of the root hairs infected at 14 DPI). Secondary infection of “Juxin” was first observed at 8 DPI, while in Bap055 and Bap246, secondary infection was first observed at 10 DPI. At 14 DPI, the percentage of cortical infection in “Juxin,” Bap055 and Bap246 was 93.3, 20.0, and 11.1%, respectively. Although cortical infection was more widespread in Bap055 than in Bap246, secondary infection in both of these hosts was restricted relative to the susceptible check, and the vascular system remained intact. A large number of binucleate secondary plasmodia were observed in “Juxin” and the vascular system was disrupted at 16 DPI; in Bap055 and Bap246, only a few secondary plasmodia were visible, with no binucleate secondary plasmodia. The defense mechanisms and expression of resistance appears to differ between Chinese cabbage cultivars carrying different sources of resistance.

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“…Finally, PI dye solution was added to stain the nuclei, and the cells were placed on ice for 30.0 min. The nuclei were visualized by a microscope [ 24 , 25 , 26 ].…”

Section: Methodsmentioning

confidence: 99%

Germplasm Enhancement and Identification of Loci Conferring Resistance against Plasmodiophora brassicae in Broccoli

Xie

Wei

Liu

et al. 2022

Genes

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In order to breed broccoli and other Brassica materials to be highly resistant to clubroot disease, 41 Brassicaceae varieties were developed and identified between 2020 and 2021. Seven known clubroot genes were used for screening these materials. In addition, the resistant and susceptible broccoli cultivars were designed for observing their differences in the infection process with Plasmodiophora brassicae. The results showed that 90% of total materials had carried more than two clubroot resistance genes: one material carried two disease resistance genes, four materials carried seven genes for clubroot resistance, two materials carried six genes for clubroot resistance, and in total 32% of these materials carried five genes for clubroot resistance. As a result, several new genotypes of Brassicaceae germplasm were firstly created and obtained based on distant hybridization and identification of loci conferring resistance against Plasmodiophora brassicae in this study. We found and revealed that similar infection models of Plasmodiophora brassicae occurred in susceptible and resistant cultivars of broccoli, but differences in infection efficiency of Plasmodiophora brassicae also existed in both materials. For resistant broccoli plants, a small number of conidia formed in the root hair, and only a few spores could enter the cortex without forming sporangia while sporangia could form in susceptible plants. Our study could provide critical Brassica materials for breeding resistant varieties and new insight into understanding the mechanism of plant resistance.

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Characterization of a Gene Identified in Pathotype 5 of the Clubroot PathogenPlasmodiophora brassicae

Cited by 22 publications

References 41 publications

Pathogen Identification of Clubroot Disease in Chinese Cabbage from Yuanyang County, Henan Province

Pathogen Identification of Clubroot Disease in Chinese Cabbage from Yuanyang County, Henan Province

Histopathology of the Plasmodiophora brassicae-Chinese Cabbage Interaction in Hosts Carrying Different Sources of Resistance

Germplasm Enhancement and Identification of Loci Conferring Resistance against Plasmodiophora brassicae in Broccoli

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