Characterization of a Hg2+-Selective Fluorescent Probe Based on Rhodamine B and Its Imaging in Living Cells

Abstract

A small organic molecule P was synthesized and characterized as a fluorometric and colorimetric dual-modal probe for Hg2+. The sensing characteristics of the proposed probe for Hg2+ were studied in detail. A fluorescent enhancing property at 583 nm (>30 fold) accompanied with a visible colorimetric change, from colorless to pink, was observed with the addition of Hg2+ to P in an ethanol-water solution (8:2, v/v, 20 mM HEPES, pH 7.0), which would be helpful to fabricate Hg2+-selective probes with “naked-eye” and fluorescent detection. Meanwhile, cellular experimental results demonstrated its low cytotoxicity and good biocompatibility, and the application of P for imaging of Hg2+ in living cells was satisfactory.

Optical and Thermal Investigations of Eutectic Metallomesogen Mixtures Based on Salicylaldiaminates Metal Complexes with a Large Nematic Stability Range

“…The studies on liquid-crystalline organo-transition metal complexes continue to generate enormous research interest for their ability to easily tune photochemical and photophysical properties. This has made them particularly promising for applications in organic electronics and sensors in various fields as photovoltaic devices [1][2][3][4], DNA binders [5][6][7][8][9][10][11], sensors [12][13][14][15], photosensitizers [16][17][18], molecular chemistry [19], fluorescent and colorimetric probes [20][21][22][23][24][25][26][27][28][29][30][31], medicinal chemistry [32] and metal-organic framework (MOF) construction [33][34][35] but also in more interesting topics of contemporary applied research such as electroluminescent displays, smart sensors, encryption systems or fuel cells but also in large varieties of metallomesogens with photoluminescence [36][37][38], electroluminescence [39][40][41][42], magnetic [43][...…”

A Naked-Eye and Fluorescent Dual-Channel Probe for Rapid Detection of Hg2+ and Its Multiple Applications

“…[17][18][19] Several fluorescent probes have been reported to detect Hg 2 ＋ , among which probes designed based on specific reactions superior to other sensing mechanisms in terms of selectivity and sensitivity, most of these probes have achieved good results in emission wavelength, water solubility and bioimaging, but further exploration is needed in reaction speed and detection of actual samples. [20][21][22][23][24][25] Therefore, it is necessary to prepare a fluorescent probe with high sensitivity that can detect lower levels of Hg 2 ＋ in actual samples and organisms. In addition, the thioacetals deprotection mechanism is considered to be a favorable option.…”