Characterization of a hypervirulent multidrug-resistant ST23 Klebsiella pneumoniae carrying a bla IncFII plasmid and a pK2044-like plasmid

Yang, Yan; Li, Xue; Zhang, Youwen; Liu, Jianhua; Hu, Xinxin; Nie, Tongying; Yang, Xinyi; Wang, Xiukun; Li, Congran; You, Xuefu

doi:10.1016/j.jgar.2020.05.004

Cited by 12 publications

(9 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, they proved that various MDR efflux pumps exist in Gram-positive bacteria ( Staphylococcus aureus and Streptococcus pneumoniae ), Gram-negative bacteria ( Pseudomonas aeruginosa and Escherichia coli ), and fungi ( Candida albicans) [ 27 ]. Furthermore, Streptococcus pyogenes [ 28 ], Enterococcus casseliflavus [ 29 , 30 ], Klebsiella pneumoniae [ 31 , 32 ], and Candida parapsilosis [ 33 ] were also included in MDR pathogens, according to other studies.…”

Section: Introductionmentioning

confidence: 99%

Elemental Analysis and In Vitro Evaluation of Antibacterial and Antifungal Activities of Usnea barbata (L.) Weber ex F.H. Wigg from Călimani Mountains, Romania

Popovici

Bucur

Calcan

et al. 2021

Plants

View full text Add to dashboard Cite

This study aims to complete our research on Usnea barbata (L.) Weber ex F.H. Wigg (U. barbata) from the Călimani Mountains, Romania, with an elemental analysis and to explore its antibacterial and antifungal potential. Thus, we analyzed twenty-three metals (Ca, Fe, Mg, Mn, Zn, Al, Ag, Ba, Co, Cr, Cu, Li, Ni, Tl, V, Mo, Pd, Pt, Sb, As, Pb, Cd, and Hg) in dried U. barbata lichen (dUB) by inductively coupled plasma mass spectrometry (ICP-MS). For the second study, we performed dried lichen extraction with five different solvents (ethyl acetate, acetone, ethanol, methanol, and water), obtaining five U. barbata dry extracts (UBDE). Then, using an adapted disc diffusion method (DDM), we examined their antimicrobial activity against seven bacterial species—four Gram-positive (Staphylococcus aureus, Enterococcus casseliflavus, Streptococcus pyogenes, and Streptococcus pneumoniae) and three Gram-negative (Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa)—and two fungi species (Candida albicans and Candida parapsilosis). Usnic acid (UA) was used as a positive control. The ICP-MS data showed a considerable Ca content (979.766 µg/g), followed by, in decreasing order, Mg, Mn, Al, Fe, and Zn. Other elements had low levels: Ba, Cu, Pb, and Cr (3.782–1.002 µg/g); insignificant amounts (<1 µg/g) of Hg and V were also found in dUB. The trace elements Ag, As, Cd, Co, Li, Tl, Mo, Pd, Pt, and Sb were below detection limits (<0.1 µg/g). The DDM results—expressed as the size (mm) of the inhibition zone diameter (IZs)—proved that the water extract did not have any inhibitory activity on any pathogens (IZs = 0 mm). Gram-positive bacteria displayed the most significant susceptibility to all other UBDE, with Enterococcus casseliflavus showing the highest level (IZs = 20–22 mm). The most susceptible Gram-negative bacterium was Pseudomonas aeruginosa (IZs = 16–20 mm); the others were insensitive to all U. barbata dry extracts (IZs = 0 mm). The inhibitory activity of UBDE and UA on Candida albicans was slightly higher than on Candida parapsilosis.

show abstract

Section: Introductionmentioning

confidence: 99%

Elemental Analysis and In Vitro Evaluation of Antibacterial and Antifungal Activities of Usnea barbata (L.) Weber ex F.H. Wigg from Călimani Mountains, Romania

Popovici

Bucur

Calcan

et al. 2021

Plants

View full text Add to dashboard Cite

show abstract

“…More importantly, key virulence-associated gene cluster has successfully transmitted into the chromosome by IS 26 within the ST11/KL47 CR-hvKp strains ( 27 ). The common way to acquire antimicrobial resistance phenotype was primarily dominated by the acquisition of various antimicrobial-resistant plasmids ( 28 – 33 ). The IS 26 also plays a great role in the formation of resistance plasmids and the insertion of resistance genes into the chromosome ( 34 ).…”

Section: Discussionmentioning

confidence: 99%

The Role of Plasmid and Resistance Gene Acquisition in the Emergence of ST23 Multi-Drug Resistant, Hypervirulent Klebsiella pneumoniae

Liu

Fan

et al. 2022

Microbiol Spectr

View full text Add to dashboard Cite

show abstract

“…Among them, sequences with the correct 5′ primers, 3′ primers, and polyA tail were full length and were further divided into chimeric and non-chimeric sequences according to their position relationships. Then, the Iso Seq module in SMRT Link software ( Yang et al, 2020 ) was used to cluster sequences similar to the full-length non-chimeric (FLNC) sequences; each cluster obtained a consensus isoform. Sequences with an accuracy >99% were high-quality transcripts, and the rest were low-quality transcripts.…”

Section: Methodsmentioning

confidence: 99%

Seed Germination Mechanism of Carex rigescens Under Variable Temperature Determinded Using Integrated Single-Molecule Long-Read and Illumina Sequence Analysis

Teng

Yue

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

The turfgrass species Carex rigescens has broad development and utilization prospects in landscaping construction. However, seed dormancy and a low germination rate have inhibited its application. Furthermore, the molecular mechanisms of seed germination in C. rigescens have not been thoroughly studied. Therefore, in the present study, PacBio full-length transcriptome sequencing combined with Illumina sequencing was employed to elucidate the germination mechanism of C. rigescens seeds under variable temperatures. In general, 156,750 full-length non-chimeric sequences, including those for 62,086 high-quality transcripts, were obtained using single-molecule long read sequencing. In total, 40,810 high-quality non-redundant, 1,675 alternative splicing, 28,393 putative coding sequences, and 1,052 long non-coding RNAs were generated. Based on the newly constructed full-length reference transcriptome, 23,147 differentially expressed genes were identified. We screened four hub genes participating in seed germination using weighted gene co-expression network analysis. Combining these results with the physiological observations, the important roles of sucrose and starch metabolic pathways in germination are further discussed. In conclusion, we report the first full-length transcriptome of C. rigescens, and investigated the physiological and transcriptional mechanisms of seed germination under variable temperatures. Our results provide valuable information for future transcriptional analyses and gene function studies of C. rigescens.

show abstract

Characterization of a hypervirulent multidrug-resistant ST23 Klebsiella pneumoniae carrying a bla IncFII plasmid and a pK2044-like plasmid

Cited by 12 publications

References 15 publications

Elemental Analysis and In Vitro Evaluation of Antibacterial and Antifungal Activities of Usnea barbata (L.) Weber ex F.H. Wigg from Călimani Mountains, Romania

Elemental Analysis and In Vitro Evaluation of Antibacterial and Antifungal Activities of Usnea barbata (L.) Weber ex F.H. Wigg from Călimani Mountains, Romania

The Role of Plasmid and Resistance Gene Acquisition in the Emergence of ST23 Multi-Drug Resistant, Hypervirulent Klebsiella pneumoniae

Seed Germination Mechanism of Carex rigescens Under Variable Temperature Determinded Using Integrated Single-Molecule Long-Read and Illumina Sequence Analysis

Contact Info

Product

Resources

About