Characterization of a Lentogenic Newcastle Disease Virus Isolated from Broiler Chickens in Japan

Murakawa, Yasushi; Takase, Kozo; Sakamoto, Kuniaki; Suesoshi, Masuo; Nagatomo, Hiroshi

doi:10.2307/1593112

Cited by 5 publications

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“…After 1985, NDV genotype VII began to replace genotype VI as the most prevalent genotype in domestic fowl in Japan [12]. Further, the more recently isolated (in 1995) lentogenic chicken isolate JP/MET/95 [13] was classified as NDV genotype II. Therefore, dk-Aomori/03 differed phylogenetically from isolates that caused outbreaks in domestic fowl in Japan.…”

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Characterization of Newcastle Disease Virus Isolated from Northern Pintail (Anas acuta) in Japan

Sakai

Sakabe

Tani

et al. 2007

The Journal of Veterinary Medical Science

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ABSTRACT. A field isolate of Newcastle disease virus (NDV) isolated from northern pintail (Anas acuta) in Tohoku district, northeast Japan, was characterized. Phylogenetic analysis of the fusion protein indicated that the isolate belonged to genotype I and was closely related to isolates from the Far East corresponded to the migration route for this bird species. The isolate had the typical avirulent cleavage site of the fusion protein 112 GKQGR*L 117 . In addition, pathogenicity tests indicated the isolate to have avirulent characteristics. However, the isolate has been shown to cause fusion cytopathic effects and form plaques on chicken embryo fibroblasts (CEF) in the absence of trypsin. The present results suggest that the CEF-adapted NDV, which is avirulent, is circulating among waterfowl populations. KEY WORDS: Newcastle disease virus (NDV), plaque formation, wild birds.

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Characterization of Newcastle Disease Virus Isolated from Northern Pintail (Anas acuta) in Japan

Sakai

Sakabe

Tani

et al. 2007

The Journal of Veterinary Medical Science

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“…The isolate of NDV was cloned and named the MET95 strain. A characterization test revealed that the MET 95 strain has very low virulence to chickens; intracerebral pathogenicity for day-old chicks, intravenous pathogenicity of 6-week-old chickens, and mean death time of the minimum lethal dose of the MET95 strain were almost the same as those of the B1 strain [5].However, we found that the HA of MET95 was different from B1 when examined by the rapid glass plate method. Namely, the MET95 strain failed to show HA when examined by rapid glass plate method but not by the micro-plate method.…”

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confidence: 63%

“…In another experiment, we found that the pathogenicity of MET95 strain to chickens was very low while the HI antibody titer of chickens infected with the MET95 strain was obviously higher than chickens infected with B1 strain of NDV [5]. Therefore, the MET95 strain may become a good candidate of vaccine strains for ND.…”

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confidence: 97%

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“…Sci. 62(12): 1325-1326, 2000 Newcastle disease virus (NDV) of Paramyxoviridae has been well known to possess hemagglutination (HA) activity on the envelope [1]. This HA has been used widely so far for identification and titration of NDV [3].…”

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Unique Hemagglutination Activity of an Isolate of Newcastle Disease Virus.

Takase

Murakawa

Eriguchi

et al. 2000

The Journal of Veterinary Medical Science

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ABSTRACT. The MET95 strain of a lentogenic Newcastle disease virus (NDV) isolated from a broiler in Japan, showed unique hemagglutination (HA) activity. The MET95 strain failed to show HA when examined by rapid glass plate method although they showed HA titer of 1:1,024 by micro-plate method. This unique HA was also observed after the MET95 strain was passaged ten times in chickens. The failure of HA by rapid glass plate method was not shown in any other NDVs examined. KEY WORDS: hemagglutination, Newcastle disease virus.J. Vet. Med. Sci. 62(12): 1325-1326, 2000 Newcastle disease virus (NDV) of Paramyxoviridae has been well known to possess hemagglutination (HA) activity on the envelope [1]. This HA has been used widely so far for identification and titration of NDV [3]. In general, NDV grows well in embryonating chicken eggs with release of a high number of virus particles in allantoic fluids, even if a small number of NDV is inoculated. The virus-existence in allantoic fluids is easily confirmed by an HA test mixing the allantoic fluid with chicken erythrocytes. This HA test has been carried out by two methods: micro-plate or rapid glass plate method [2,6].In 1995, an NDV was isolated from the lung/trachea material of a 28-day-old broiler which had been reared in a flock with about 20,000 pen-mates which were not given any vaccines after hatching [4]. The isolate of NDV was cloned and named the MET95 strain. A characterization test revealed that the MET 95 strain has very low virulence to chickens; intracerebral pathogenicity for day-old chicks, intravenous pathogenicity of 6-week-old chickens, and mean death time of the minimum lethal dose of the MET95 strain were almost the same as those of the B1 strain [5].However, we found that the HA of MET95 was different from B1 when examined by the rapid glass plate method. Namely, the MET95 strain failed to show HA when examined by rapid glass plate method but not by the micro-plate method. In this paper, this unique HA of MET95 is described compared with the other NDVs.Eight strains of NDVs were used in the experiments. The MET95 strain was plaque-cloned three times in chicken kidney cell cultures and passaged four times in specific pathogen-free (SPF) embryonating chicken eggs after isolation. The MET95(p10) strain was obtained from the MET95 strain after 10 times of passage in SPF chickens. The B1 strain is a live vaccine strain which has been used worldwide. The Sato strain is a virulent strain which has been used as a challenge virus for vaccine efficacy tests of the national assay test in Japan. The Atami and Fuji strains are virulent NDVs derived from the Japanese Association of Veterinary Biologics (Tokyo). These NDVs were propagated in SPF chicken eggs and their allantoic fluids were used as virus material.The VG/GA and La Sota clone 30 strains are vaccine viruses purchased from Merial Japan Ltd. (Tokyo) and Intervet K. K. (Tokyo), respectively. Each vaccine was rehydrated with the accompanying liquid diluent and used as virus material.For red blood cell sol...

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Newcastle disease

Behboudi

2023

CABI Compendium

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Characterization of a Lentogenic Newcastle Disease Virus Isolated from Broiler Chickens in Japan

Cited by 5 publications

References 2 publications

Characterization of Newcastle Disease Virus Isolated from Northern Pintail (Anas acuta) in Japan

Characterization of Newcastle Disease Virus Isolated from Northern Pintail (Anas acuta) in Japan

Unique Hemagglutination Activity of an Isolate of Newcastle Disease Virus.

Newcastle disease

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