Characterization of a method for measuring primary hyperalgesia of deep somatic tissue

Skyba, David A.; Radhakrishnan, Rajan; Sluka, Kathleen A.

doi:10.1016/j.jpain.2004.10.002

Cited by 80 publications

(79 citation statements)

References 21 publications

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“…That is why despite the reported analgesic effects of XAE and XA (Woode et al 2012(Woode et al , 2013Ameyaw et al 2014), the current study evaluated their specific potential in reducing musculoskeletal pain. The acute and chronic musculoskeletal pain models used in this study are more closely related to fibromyalgia and arthritic pain experienced in humans (Skyba et al 2005).…”

Section: Discussionmentioning

confidence: 99%

“…Morphine served as reference antinociceptive agent due to its effects on both central and peripheral transmission of pain. The analgesic effect of XAE and XA were evaluated in acute skeletal pain models similar to that described (Skyba et al 2005) with slight modification. On the test day, baseline compression thresholds of the ipsilateral knees were taken with an analgesimeter (IITC Life Science Inc. Model 2888, Woodland Hills, CA) by compressing the knee of the rat until the animal withdrew the limb forcefully or vocalized.…”

Section: Assessment Of the Effect Of Xae And Xa On Acute Skeletal Painmentioning

confidence: 99%

“…Assessment of the analgesic properties of XAE and XA on acute muscle pain was done by measuring hind limb grip strength of rats with a grip force analyzer; as described earlier (Kehl et al 2003;Skyba et al 2005;Tillu et al 2008). The force analyzer measured the amount of tensile force each rat exerted against a wire mesh grid with its hind paws when pulled gently in the caudal direction.…”

Section: Analgesic Effects Of Xae and Xa Acute Muscle Painmentioning

confidence: 99%

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Effects of an ethanol extract and the diterpene, xylopic acid, of Xylopia aethiopica fruits in murine models of musculoskeletal pain

Woode

Ameyaw

Boakye‐Gyasi

et al. 2016

Pharmaceutical Biology

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Context: Fruits of Xylopia aethiopica (Dunal) A. Rich. (Annonaceae) are used traditionally to manage arthritis, headache and other pain disorders. Objective: The analgesic properties of the X. aethiopica ethanol fruit extract (XAE) and xylopic acid (XA) were evaluated in musculoskeletal pain models. Materials and methods: Acute muscle pain was induced in gastrocnemius muscle of Sprague-Dawley rats with 3% carrageenan (i.m.). Rats received XAE (30-300 mg/kg), XA (10-100 mg/kg) or morphine (1-10 mg/ kg) after 12 h. Effects of XAE and XA on muscle pain were assessed by measuring post-treatment grip strength of the rats. Chronic muscle pain was similarly induced, but drug treatment was on the eighth day and effects of XAE and XA assessed with Randall-Selitto test for hyperlagesia. Acute-skeletal pain was induced in knee joints of rats with 3% carrageenan-kaolin mixture and effects determined 12-h later. Similar induction protocol was used for chronic knee pain with treatment and measurement as done for chronic muscle pain. Results: XAE and XA significantly and dose-dependently ameliorated both acute muscle (ED 50 mg/kg: XAE ¼ 22.9; XA ¼ 6.2) and skeletal hyperalgesia (XAE ¼ 39.9; XA ¼ 17.7) induced by 3% carrageenan. Similarly, chronic skeletal hyperalgesia was reduced by XAE and XA treatment similar to morphine (ED 50 : XAE ¼ 13.0; XA ¼ 4.6). This reduction was also seen in chronic muscle hyperalgesia (ED 50 : XAE¼ 79.1; XA ¼ 42.7). XAE and XA significantly reduced the spread of hyperalgesia to contralateral limbs in both models of chronic hyperalgesia. Conclusion: These findings establish analgesic properties of the ethanol fruit extract of X. aethiopica and xylopic acid in musculoskeletal pain. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

Section: Assessment Of the Effect Of Xae And Xa On Acute Skeletal Painmentioning

confidence: 99%

Section: Analgesic Effects Of Xae and Xa Acute Muscle Painmentioning

confidence: 99%

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Effects of an ethanol extract and the diterpene, xylopic acid, of Xylopia aethiopica fruits in murine models of musculoskeletal pain

Woode

Ameyaw

Boakye‐Gyasi

et al. 2016

Pharmaceutical Biology

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“…6 Rats were also tested for knee joint withdrawal threshold with a pair of forceps applied to the knee joint as previously described for the gastrocnemius muscle. 29 The forceps were equipped with 2 strain gauges to measure force. To measure knee joint withdrawal threshold, animals were placed in the restrainer (glove), and the experimenter compressed the knee joint with the tip of the forceps while the hind limb was extended.…”

Section: Behavioral Testingmentioning

confidence: 99%

Modulation Between High- and Low-Frequency Transcutaneous Electric Nerve Stimulation Delays the Development of Analgesic Tolerance in Arthritic Rats

DeSantana

Filho

Sluka

2008

Archives of Physical Medicine and Rehabilitation

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Objective-To investigate whether repeated administration of modulating frequency transcutaneous electric nerve stimulation (TENS) prevents development of analgesic tolerance.Design-Knee joint inflammation (3% carrageenan and kaolin) was induced in rats. Either mixed or alternating frequency was administered daily (20min) for 2 weeks to the inflamed knee under light halothane anesthesia (1%-2%). Setting-Laboratory. Animals-Adult male Sprague-Dawley rats (N=36).Intervention-Mixed-(4Hz and 100Hz) or alternating-(4Hz on 1 day; 100Hz on the next day) frequency TENS at sensory intensity and 100μs pulse duration.Main Outcome Measures-Paw and joint withdrawal thresholds to mechanical stimuli were assessed before induction of inflammation, and before and after daily application of TENS.Results-The reduced paw and joint withdrawal thresholds that occur 24 hours after the induction of inflammation were significantly reversed by the first administration of TENS when compared with sham treatment or to the condition before TENS treatment, which was observed through day 9. By the tenth day, repeated daily administration of either mixed-or alternating-frequency TENS did not reverse the decreased paw and joint withdrawal thresholds.Conclusions-These data suggest that repeated administration of modulating frequency TENS leads to a development of opioid tolerance. However, this tolerance effect is delayed by approximately 5 days compared with administration of low-or high-frequency TENS independently. Clinically, we can infer that a treatment schedule of repeated daily TENS administration will result in a tolerance effect. Moreover, modulating low and high frequency TENS seems to produce a better analgesic effect and tolerance is slower to develop.

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“…The compression withdrawal threshold of the knee was measured as previously described (Skyba et al, 2005). Rats were acclimated to the restraining device for 5 min 3 times per day for 2 consecutive days.…”

Section: Measurement Of Compression Withdrawal Threshold Of the Kneementioning

confidence: 99%

Release of GABA and activation of GABAA in the spinal cord mediates the effects of TENS in rats

et al. 2007

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Transcutaneous electrical nerve stimulation (TENS) is a commonly utilized non-pharmacological, non-invasive treatment for pain. GABA is a neurotransmitter in the dorsal horn of the spinal cord that mediates analgesia locally, and also through activation of supraspinal sites. TENS reduces hyperalgesia through activation of receptor-mediated pathways at the level of the spinal cord, and supraspinally. The current study tested the hypothesis that either high or low frequency TENS applied to the inflamed knee joint increases GABA in the spinal cord dorsal horn and activates GABA receptors spinally. We utilized microdialysis to sample the extracellular fluid before, during and after TENS and analyzed GABA in dialysates with high performance liquid chromatography. We analyzed the extracellular GABA concentrations in animals with and without knee joint inflammation induced by intra-articular injection of kaolin and carrageenan. We further tested if spinal blockade of GABA receptors prevents the antihyperalgesia produced by TENS in rats with joint inflammation. We show that high frequency TENS increases extracellular GABA concentrations in the spinal cord in animals with and without joint inflammation. The increases in GABA do not occur in response to low frequency TENS, and there are no increases in glycine in response to low or high frequency TENS. However, the reduction in primary hyperalgesia by both high and low frequency TENS is prevented by spinal blockade of GABA A receptors with bicuculline. Thus, high frequency TENS increases release of GABA in the deep dorsal horn of the spinal cord, and both high and low frequency TENS reduce primary hyperalgesia by activation of GABA A receptors spinally.

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Characterization of a method for measuring primary hyperalgesia of deep somatic tissue

Cited by 80 publications

References 21 publications

Effects of an ethanol extract and the diterpene, xylopic acid, of Xylopia aethiopica fruits in murine models of musculoskeletal pain

Effects of an ethanol extract and the diterpene, xylopic acid, of Xylopia aethiopica fruits in murine models of musculoskeletal pain

Modulation Between High- and Low-Frequency Transcutaneous Electric Nerve Stimulation Delays the Development of Analgesic Tolerance in Arthritic Rats

Release of GABA and activation of GABAA in the spinal cord mediates the effects of TENS in rats

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