Characterization of a microbial community capable of reducing perchlorate and nitrate in high salinity

Abstract

Denitrifying up-flow packed-bed bioreactors fed with perchlorate and nitrate allowed for the examination of the impact of a variety of salt conditions (up to 10% w/v NaCl) on the complete perchlorate and nitrate removal capacity of the reactor using activated sludge taken from a municipal wastewater treatment plant. Based on the evaluation of the microbial community in the bioreactor by cloning analysis, Clostridium sp. and a Rhodocyclaceae bacteria were identified as the dominant clones. This suggests that they may be tolerant to high salt and can reduce both nitrate and perchlorate in such conditions.

Purification, Characterization, and Potential of Saline Waste Water Remediation of a Polyextremophilicα-Amylase from an Obligate HalophilicAspergillus gracilis

“…The results show that increasing salinity increased the performance of amylase from A. gracilis TISTR 3638, while it decreased the performance of commercial amylase. The use of halophilic enzymes has already been reported for the bioremediation of saline areas as well as for treatment of saline waste water [43, 44]. As our enzyme has been found more salt loving than reported halophilic amylases, it provides the possibility of using it in saline and low water activity waste management.…”

Purification and Characterization of Extracellular, Polyextremophilic α-amylase Obtained from Halophilic Engyodontium album

“…This capability is much greater than the previously reported bacterial and Archeal amylases (6,9,30). Ability of this amylase to work at extreme salt concentrations makes them the best available candidate to work for bioremediation processes, particularly, saline waste management (31,32). Similarly the limiting water resources and availability of hard water for domestic uses in current climate change, requires the additive amylases for detergents to work better in low water activity for minimizing the consumption of water, as well as to make more cleaning effects in the presence of high salt concentrations (33).…”

Perchlorate reduction by an isolatedSerratia marcescensstrain under high salt and extreme pH

“…Furthermore, cloning and sequencing have revealed Clostridium sp. and Rhodocyclaceae as dominant ‐degrading population in a packed‐bed bioreactor at 10% NaCl (Chung et al ., ). The maximum salinity tolerance in perchlorate‐reducing bacteria reported so far was 11% by an acclimatized mixed culture (Logan et al ., ).…”