Characterization of a myostatin gene (MSTN1) from spotted halibut (Verasper variegatus) and association between its promoter polymorphism and individual growth performance

Li, Hongjun; Fan, Jingfeng; Liu, Shuxi; Yang, Qing; Mu, Guiqiang; He, Chongbo

doi:10.1016/j.cbpb.2011.12.008

Cited by 28 publications

(8 citation statements)

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“…MSTN, also known as growth and differentiation factor 8 (GDF8), is a negative regulator of vertebrate muscle growth. MSTN SNPs are significantly associated with growth traits in the commercial scallop [18], bighead carp ( Aristichthys nobilis ) [38], and spotted halibut ( Verasper variegatus ) [39]. In this study, we identified a number of growth-related genes, including growth factors, growth factor receptors, and growth factor-binding proteins ( Table 2 ), which have been rarely reported in bivalves.…”

Section: Resultsmentioning

confidence: 90%

Development of Molecular Resources for an Intertidal Clam, Sinonovacula constricta, Using 454 Transcriptome Sequencing

et al. 2013

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BackgroundThe razor clam Sinonovacula constricta is a benthic intertidal bivalve species with important commercial value. Despite its economic importance, knowledge of its transcriptome is scarce. Next generation sequencing technologies offer rapid and efficient tools for generating large numbers of sequences, which can be used to characterize the transcriptome, to develop effective molecular markers and to identify genes associated with growth, a key breeding trait.ResultsTotal RNA was isolated from the mantle, gill, liver, siphon, gonad and muscular foot tissues. High-throughput deep sequencing of S. constricta using 454 pyrosequencing technology yielded 859,313 high-quality reads with an average read length of 489 bp. Clustering and assembly of these reads produced 16,323 contigs and 131,346 singletons with average lengths of 1,376 bp and 458 bp, respectively. Based on transcriptome sequencing, 14,615 sequences had significant matches with known genes encoding 147,669 predicted proteins. Subsequently, previously unknown growth-related genes were identified. A total of 13,563 microsatellites (SSRs) and 13,634 high-confidence single nucleotide polymorphism loci (SNPs) were discovered, of which almost half were validated.ConclusionDe novo sequencing of the razor clam S. constricta transcriptome on the 454 GS FLX platform generated a large number of ESTs. Candidate growth factors and a large number of SSRs and SNPs were identified. These results will impact genetic studies of S. constricta.

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Section: Resultsmentioning

confidence: 90%

Development of Molecular Resources for an Intertidal Clam, Sinonovacula constricta, Using 454 Transcriptome Sequencing

et al. 2013

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“…Such expression patterns are suggestive of a functional divergence among fishes, as the single mammalian myostatin ortholog is predominately expressed in muscle. To identify potential mechanisms responsible for changes in spatial and temporal expression patterns, numerous studies have investigated the promoter region of myostatin in mammals and fish to characterize putative transcription factor binding sites and hormone response elements (Allen and Du, 2008; Funkenstein et al, 2009b; Gabillard et al, 2013; Garikipati et al, 2007; Garikipati et al, 2006; Hu et al, 2013; Li et al, 2012a; Li et al, 2012b; Li et al, 2012c; Ma et al, 2001a; Nadjar-Boger et al, 2012; Nadjar-Boger et al, 2013; Ostbye et al, 2007a; Rodgers and Garikipati, 2008; Spiller et al, 2002; Xue et al, 2012). Putative E-box protein, myogenic regulatory factor (MRF), and myocyte enhancing factor (MEF) binding motifs and glucocorticoid response elements (GREs) appear to be highly conserved components of the myostatin promoter in vertebrates (Rodgers and Garikipati, 2008).…”

Section: Introductionmentioning

confidence: 99%

The effects of exogenous cortisol on myostatin transcription in rainbow trout, Oncorhynchus mykiss

Galt

Froehlich

Remily

et al. 2014

Comparative Biochemistry and Physiology Part A: Molecular &

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Glucocorticoids (GCs) strongly regulate myostatin transcript levels in mammals via glucocorticoid response elements (GREs) in the myostatin promoter, and bioinformatics methods suggest that this regulatory mechanism is conserved among many vertebrates. However, the multiple myostatin genes found in some fishes may be an exception. In rainbow trout (Oncorhynchus mykiss), two genome duplication events have produced three putatively functional myostatin genes, myostatin-1a, -1b and -2a, which are ubiquitously and differentially expressed. In addition, in silico promoter analyses of the rainbow trout myostatin promoters have failed to identify putative GREs, suggesting a divergence in myostatin function. Therefore, we hypothesized that myostatin mRNA expression is not regulated by glucocorticoids in rainbow trout. In this study, both juvenile rainbow trout and primary trout myoblasts were treated with cortisol to examine the relationship between this glucocorticoid and myostatin mRNA expression. Results suggest that exogenous cortisol does not regulate myostatin-1a and -1b expression in vivo, as myostatin mRNA levels were not significantly affected by cortisol treatment in either red or white muscle tissue. In red muscle, myostatin-2a levels were significantly elevated in the cortisol treatment group relative to the control, but not the vehicle control, at both 12 h and 24 h post-injection. As such, it is unclear if cortisol was acting alone or in combination with the vehicle. Cortisol increased myostatin-1b expression in a dose-dependent manner in vitro. Further work is needed to determine if this response is the direct result of cortisol acting on the myostatin-1b promoter or through an alternative mechanism. These results suggest that regulation of myostatin by cortisol may not be as highly conserved as previously thought and support previous work that describes potential functional divergence of the multiple myostatin genes in fishes.

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“…In mammals, mstn gene is known to act as potent regulator of muscle growth, and a mstn-knockout fish highly increased muscle mass (de Santis et al, 2012;. Contrarily to mammals, mstn transcription in fish species is ubiquitous expressed indicating an involvement in other physiological mechanisms as well as in skeletal muscle growth regulation (Campos et al, 2010;Li et al, 2012;Canada et al, 2016). Different stress situations that reduced fish growth were shown not to be correlated with the expression of mstn (Rescan, 2005).…”

Section: Discussionmentioning

confidence: 99%

Dietary Antioxidant Supplementation Promotes Growth in Senegalese Sole Postlarvae

et al. 2020

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Somatic growth is a balance between protein synthesis and degradation, and it is largely influenced by nutritional clues. Antioxidants levels play a key role in protein turnover by reducing the oxidative damage in the skeletal muscle, and hence promoting growth performance in the long-term. In the present study, Senegalese sole postlarvae (45 days after hatching, DAH) were fed with three experimental diets, a control (CTRL) and two supplemented with natural antioxidants: curcumin (CC) and grape seed (GS). Trial spanned for 25 days and growth performance, muscle cellularity and the expression of muscle growth related genes were assessed at the end of the experiment (70 DAH). The diets CC and GS significantly improved growth performance of fish compared to the CTRL diet. This enhanced growth was associated with larger muscle cross sectional area, with fish fed CC being significantly different from those fed the CTRL. Sole fed the CC diet had the highest number of muscle fibers, indicating that this diet promoted muscle hyperplastic growth. Although the mean fiber diameter did not differ significantly amongst treatments, the proportion of large-sized fibers (>25 μm) was also higher in fish fed the CC diet suggesting increased hypertrophic growth. Such differences in the phenotype were associated with a significant up-regulation of the myogenic differentiation 2 ( myod2 ) and the myomaker ( mymk ) transcripts involved in myocyte differentiation and fusion, respectively, during larval development. The inclusion of grape seed extract (GS diet) resulted in a significant increase in the expression of myostatin1 . These results demonstrate that both diets (CC and GS) can positively modulate muscle development and promote growth in sole postlarvae. This effect is more prominent in CC fed fish, where increased hyperplastic and hypertrophic growth of the muscle was associated with an upregulation of myod2 and mymk genes.

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Characterization of a myostatin gene (MSTN1) from spotted halibut (Verasper variegatus) and association between its promoter polymorphism and individual growth performance

Cited by 28 publications

References 45 publications

Development of Molecular Resources for an Intertidal Clam, Sinonovacula constricta, Using 454 Transcriptome Sequencing

Development of Molecular Resources for an Intertidal Clam, Sinonovacula constricta, Using 454 Transcriptome Sequencing

The effects of exogenous cortisol on myostatin transcription in rainbow trout, Oncorhynchus mykiss

Dietary Antioxidant Supplementation Promotes Growth in Senegalese Sole Postlarvae

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