Characterization of a New Defensin from Cowpea (Vigna unguiculata (L.) Walp.)

Padovan, Lara; Segat, Ludovica; Tossi, Alessandro; Calsa, Tercílio; Kido, Ederson Akio; Brandão, Lucas; Guimarães, Rafael Lima; Pandolfi, Valesca; Pestana-Calsa, Maria Clara; Belarmino, L. C.; Benko‐Iseppon, Ana Maria; Crovella, Sérgio

doi:10.2174/092986610790780350

Cited by 18 publications

(12 citation statements)

References 28 publications

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“…They play an important role in the protection of germinating plant seeds, developing seedlings and reproductive structures of plants [42-44] and have been isolated from roots [44-46], vegetative tissues and reproductive structures such as flowers and fruits [45,47-55]. The majority of characterized plant defensins show a constitutive pattern of expression, with an induction in expression in response to pathogen attack, wounding and some abiotic stresses [20,44-46]. Recently it was shown that pathogen-induced expression of Arabidopsis plant defensins is dependent on ENHANCED DISEASE RESISTANCE1 (EDR1), which interferes with the repressor function of MYC2 allowing for defensin gene expression [56].…”

Section: Introductionmentioning

confidence: 99%

Four plant defensins from an indigenous South African Brassicaceae species display divergent activities against two test pathogens despite high sequence similarity in the encoding genes

Beer

Vivier

2011

BMC Res Notes

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BackgroundPlant defensins are an important component of the innate defence system of plants where they form protective antimicrobial barriers between tissue types of plant organs as well as around seeds. These peptides also have other activities that are important for agricultural applications as well as the medical sector. Amongst the numerous plant peptides isolated from a variety of plant species, a significant number of promising defensins have been isolated from Brassicaceae species. Here we report on the isolation and characterization of four defensins from Heliophila coronopifolia, a native South African Brassicaceae species.ResultsFour defensin genes (Hc-AFP1-4) were isolated with a homology based PCR strategy. Analysis of the deduced amino acid sequences showed that the peptides were 72% similar and grouped closest to defensins isolated from other Brassicaceae species. The Hc-AFP1 and 3 peptides shared high homology (94%) and formed a unique grouping in the Brassicaceae defensins, whereas Hc-AFP2 and 4 formed a second homology grouping with defensins from Arabidopsis and Raphanus. Homology modelling showed that the few amino acids that differed between the four peptides had an effect on the surface properties of the defensins, specifically in the alpha-helix and the loop connecting the second and third beta-strands. These areas are implicated in determining differential activities of defensins. Comparing the activities after recombinant production of the peptides, Hc-AFP2 and 4 had IC50 values of 5-20 μg ml-1 against two test pathogens, whereas Hc-AFP1 and 3 were less active. The activity against Botrytis cinerea was associated with membrane permeabilization, hyper-branching, biomass reduction and even lytic activity. In contrast, only Hc-AFP2 and 4 caused membrane permeabilization and severe hyper-branching against the wilting pathogen Fusarium solani, while Hc-AFP1 and 3 had a mild morphogenetic effect on the fungus, without any indication of membrane activity. The peptides have a tissue-specific expression pattern since differential gene expression was observed in the native host. Hc-AFP1 and 3 expressed in mature leaves, stems and flowers, whereas Hc-AFP2 and 4 exclusively expressed in seedpods and seeds.ConclusionsTwo novel Brassicaceae defensin sequences were isolated amongst a group of four defensin encoding genes from the indigenous South African plant H. coronopifolia. All four peptides were active against two test pathogens, but displayed differential activities and modes of action. The expression patterns of the peptide encoding genes suggest a role in protecting either vegetative or reproductive structures in the native host against pathogen attack, or roles in unknown developmental and physiological processes in these tissues, as was shown with other defensins.

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Section: Introductionmentioning

confidence: 99%

Four plant defensins from an indigenous South African Brassicaceae species display divergent activities against two test pathogens despite high sequence similarity in the encoding genes

Beer

Vivier

2011

BMC Res Notes

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“…Antimicrobial peptides (AMPs) are important components of the first line defense of innate immune systems in various species, including mammals, amphibians, insects and plants (Broekaert et al 1995;Thomma et al 2002;Castro and Fontes 2005;Senyürek et al 2009;Padovan et al 2010). AMPs show broad-spectrum antimicrobial activity against a wide range of pathogens (Zasloff 2002;Senyürek et al 2009), and their mode of action is not completely understood (Brodgen 2005).…”

Section: Introductionmentioning

confidence: 99%

Capsicum annuum L. trypsin inhibitor as a template scaffold for new drug development against pathogenic yeast

Ribeiro

Silva

Cunha

et al. 2011

Antonie van Leeuwenhoek

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A 6,000 Da peptide, named CaTI, was isolated from Capsicum annuum L. seeds and showed potent inhibitory activity against trypsin and chymotrypsin. The aim of this study was to determine the effect of CaTI on Saccharomyces cerevisiae, Candida albicans, Candida tropicalis and Kluyveromyces marxiannus cells. We observed that CaTI inhibited the growth of S. cerevisiae, K. marxiannus as well as C. albicans and induced cellular agglomeration and the release of cytoplasmic content. No effect on growth was observed in C. tropicalis but morphological changes were noted. In the spot assay, different degrees of sensitivity were shown among the strains and concentrations tested. Scanning electron microscopy showed that S. cerevisiae, K. marxiannus and C. albicans, in the presence of CaTI, exhibited morphological alterations, such as the formation of pseudohyphae, cellular aggregates and elongated forms. We also show that CaTI induces the generation of nitric oxide and interferes in a dose-dependent manner with glucose-stimulated acidification of the medium mediated by H(+)-ATPase of S. cerevisiae cells.

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“…Sequence, amino acid arrangement, splicing analysis, secondary and tertiary structures of mature putative defensin peptide showed similarity towards a typical defensin peptide. Response of biotic stimuli on pathogen treated and untreated plants were examined by the expression level of defensin gene, using RT-PCR 63 . A list of defensin peptides, their origin and action against specific microorganisms is listed in Table 2.…”

Section: Six Main Families Of Pampsmentioning

confidence: 99%

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2018

IJPSR

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Characterization of a New Defensin from Cowpea (Vigna unguiculata (L.) Walp.)

Cited by 18 publications

References 28 publications

Four plant defensins from an indigenous South African Brassicaceae species display divergent activities against two test pathogens despite high sequence similarity in the encoding genes

Four plant defensins from an indigenous South African Brassicaceae species display divergent activities against two test pathogens despite high sequence similarity in the encoding genes

Capsicum annuum L. trypsin inhibitor as a template scaffold for new drug development against pathogenic yeast

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