Characterization of a New Infectious Bronchitis Virus Isolate II. Some Chemical and Physical Properties of Clark 333

Cowen, B. S.; Hitchner, S. B.; Ubertini, Tito

doi:10.2307/1588729

Cited by 6 publications

(11 citation statements)

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“…CU~IXG~{AM et al (5,17) have shown that trypsin inactivates several United States strains of IBV. However, the results obtained in the present investigation and those obtained by others (4,9,21,22) show that most IBV strains are trypsinresistant.…”

Section: Diseussionsupporting

confidence: 53%

“…Avian infectious bronchitis virus (IBV) is the most intensively studied coronavirus. The sensitivities of some IBV strains, for example Beaudette, Massachusetts, and Connecticut, to chemical and physical treatments have been thouroughly examined (4,6,13,14,17,21,22,23), but the results have differed from one report to another. Contradictory results have been particularly reported on the resistance of the strains to exposure to 56 ° C (6,9,13,17,18,22), to pH 3.0 (3,4,7,9,12,18,20), to pH 12.0 (18,20), to trypsin (4,5,9,17,21,22), and to ether (9,12,14,18,20).…”

Section: Introductionmentioning

confidence: 99%

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Studies on avian infectious bronchitis virus (IBV)

Otsuki

Yamamoto

Tsubokura

1979

Archives of Virology

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The resistance of avian infectious bronchitis virus (IBV) to several chemical and physical treatments was studied. Ten strains, including four Japanese strains, were used. 1. All strains were sensitive to heating at 56 degrees C for 15 minutes; although two of them, KH and Massachusetts-41, were resistant to heating at 45 degrees C for 90 minutes. 2. All strains were resistant to pH 3.0 and most of the strains were sensitive to pH 11.0. 3. All strains were completely inactivated by chloroform and sodium deoxycholate and all except Beaudette-42 and Connaught were relatively stable to ether. 4. All strains rapidly lost their infectivities upon ultraviolet irradiation. 5. Trypsin did not affect the infectivity of any strain. 6. From these results, the ten strains were classified into three groups based on their stabilities to exposure to heating at 45 degrees C for 90 minutes and to ether.

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Section: Diseussionsupporting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Studies on avian infectious bronchitis virus (IBV)

Otsuki

Yamamoto

Tsubokura

1979

Archives of Virology

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“…Moreover, TGEV strains were generally heat-labile since they were inactivated in a few days at 37°C and in 10 minutes at 56°C (3,15,18). Interestingly, Coria (5) reported that 1 ~ magnesium sulphate could protect IBV from thermal inactivation at pH values ranging from ¢ to 10 whereas Cowen et al (7) found that this virus was inactivated at high temperature (50-56 ° C) with or without 1 5~ magnesium chloride. Finally, sialodaeryoadenitis virus (SDAV), a possible member of the coronavirus group (17,24), was characterized by Bhatt et al (2), who found it to be relatively acidresistant and heat-labile.…”

Section: Discussionmentioning

confidence: 99%

“…These studies showed that pH and temperature stabilities were variable among different members of the coronavirus group, according to the strains used. For instance, some strains of the avian int~ctious bronchitis virus (IBV) were stable to acid pH whereas others were labile (1,6,7,8,26). On the other hand, transmissible gastroenteritis virus (TGEV) has been reported to be stable for at least one hour at 37°C between pH 4 and 8 (18).…”

mentioning

confidence: 99%

Physico-chemical properties of murine hepatitis virus, strain A59

Chartier

Talbot

1987

Archives of Virology

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The infectivity of murine hepatitis virus (MHV-A59) was optimally stable at pH 6.0 and was unaffected by ionic strength or at least 15 cycles of freezing and thawing. It was completely inactivated within 25 minutes at 56 degrees C, but was protected by 1 M magnesium chloride or magnesium sulphate. It was completely inactivated within 14 days at 37 and 22 degrees C, but was relatively stable for as long as 72 days at 4 degrees C and optimal pH.

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“…On the other hand, some properties appear to be common to a large number of coronaviruses. For example, most coronaviruses seem to show some decrease of infectivity when exposed to acid pHs at 37°C (Cowen et al 1971 ;Hierholzer 1976) but appear to be relatively stable at 4°C (Cheever et al 1949;Daniel and Talbot 1987).…”

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confidence: 99%

Effect of pH and temperature on the infectivity of human coronavirus 229E

Lamarre¹,

Talbot²

1989

Can. J. Microbiol.

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The stability of human coronavirus 229E infectivity was maximum at pH 6.0 when incubated at either 4 or 33 °C. However, the influence of pH was more pronounced at 33 °C. Viral infectivity was completely lost after a 14-day incubation period at 22, 33, or 37 °C but remained relatively constant at 4 °C for the same length of time. Finally, the infectious titer did not show any significant reduction when subjected to 25 cycles of thawing and freezing. These studies will contribute to optimize virus growth and storage conditions, which will facilitate the molecular characterization of this important pathogen.Key words: coronavirus, pH, temperature, infectivity, human coronavirus.

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Characterization of a New Infectious Bronchitis Virus Isolate II. Some Chemical and Physical Properties of Clark 333

Cited by 6 publications

References 0 publications

Studies on avian infectious bronchitis virus (IBV)

Studies on avian infectious bronchitis virus (IBV)

Physico-chemical properties of murine hepatitis virus, strain A59

Effect of pH and temperature on the infectivity of human coronavirus 229E

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