Characterization of a new reversed-phase chromatographic column on a 2-μm porous microspherical silica gel

C18 Modified Monolith Silica Particles of 3-5 μm

“…in this study were inferior to those obtained with commercially available conventional columns (4.6 mm I.D.) reported in the literature 15,16 probably owing to the fact that home-made microcolumns of a narrow I.D. were used in this study.…”

Chromatographic Determination of Metallic Impurities in Reversed-Phase HPLC Columns

“…Others used different chelates such as dihydroxyanthraquinones, Hinokitiol (4-isopropylcycloheptatrienolone), or aromatic dihydroxy compounds, where only those with 1,2- or 1,2,3-substituents form complexes. − Recently, a test was described comparing the plate counts of 2,3- and 2,7-dihydroxynaphthalene (DERT test, dihydroxynaphthalene efficiency ratio test) . Other complexing agents such as 8-hydroxyquinoline (oxine), diphenhydramine, and the copper oxinate chelate had been proposed, − but because of their high basicity, they may also interact strongly with surface silanols, and differentiation between silanophilic and metallophilic influences may be ambiguous.…”

Fast separation of intact proteins with high resolution under high sample loading using non-porous RPLC packings

“…The application of non-porous packings is thus expandable, according to this methodology. It may be possible to achieve complete separation of proteins on a milligram scale within less than 1 min if the dead volume of the flow path and the detection system of a conventional chromatograph decrease and smaller non-porous silica RPLC packings are used, which might thereby facilitate practical applications [10].…”