Characterization of a Novel Close-to-Root Papillomavirus from a Florida Manatee by Using Multiply Primed Rolling-Circle Amplification:
            <i>Trichechus manatus latirostris</i>
            Papillomavirus Type 1

Rector, Annabel; Bossart, Gregory D.; Ghim, Shin-je; Sundberg, John P.; Jenson, A. Bennett; Ranst, Marc Van

doi:10.1128/jvi.78.22.12698-12702.2004

Cited by 76 publications

(64 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Studies have shown that PVs may have coevolved and cospeciated with their hosts (Chan et al, 1997;Rector et al, 2007). Rector et al (2004) suggested that TmPV1 was not transmitted to manatees from other species, but rather coevolved with the species itself.…”

Section: Discussionmentioning

confidence: 99%

“…The integrity of the insert was confirmed by sequencing. The recombinant baculovirus (rL1bac) was produced by cotransfection of the recombinant transfer vector and the baculovirus-linearized DNA into Spodoptera frugiperda (Sf9) cells according to the manufacturer's recommendation (Bac-N-Blue TM Kit; Invitrogen) and as previously described (Ghim et al, 2004). The L1 expression in the Sf9 cells was confirmed by immunofluorescence.…”

Section: Tmpv1 L1 Cloning Expression and Detectionmentioning

confidence: 99%

“…However, some PVs, such as high-risk human papillomaviruses (HPVs), may be associated with cancer development (zur Hausen, 2009). Papillomaviruses have been found in a wide range of mammals (Giri et al, 1985;Garland, 2002;Ghim et al, 2004;Zaugg et al, 2005;Rector et al, 2006;Nasir and Campo, 2008). Recently, these viruses have been detected in three marine mammal species: Phocoena spinipinnis papillomavirus type 1 (PsPV1) from Burmeister's porpoises (Van Bressem et al, 2007); Tursiops truncatus papillomavirus type 1, 2, and 3 (TtPV1, -2, and -3) from bottlenose dolphins Rector et al, 2008), and Trichechus manatus latirostris (Tm) papillomavirus type 1 (TmPV1) from Florida manatees (Bossart et al, 2002;Rector et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Papillomaviruses have been found in a wide range of mammals (Giri et al, 1985;Garland, 2002;Ghim et al, 2004;Zaugg et al, 2005;Rector et al, 2006;Nasir and Campo, 2008). Recently, these viruses have been detected in three marine mammal species: Phocoena spinipinnis papillomavirus type 1 (PsPV1) from Burmeister's porpoises (Van Bressem et al, 2007); Tursiops truncatus papillomavirus type 1, 2, and 3 (TtPV1, -2, and -3) from bottlenose dolphins Rector et al, 2008), and Trichechus manatus latirostris (Tm) papillomavirus type 1 (TmPV1) from Florida manatees (Bossart et al, 2002;Rector et al, 2004). Several studies suggested the possible existence of additional marine mammal PVs involved in the etiology of cutaneous and mucosal lesions in cetaceans, although studies to isolate and characterize these PVs have not been conducted (Flom et al, 1980;Lambertsen et al, 1987;Van Bressem et al, 1999, Bossart et al, 2005Newman and Smith, 2006).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

SEROEPIDEMIOLOGY OF TmPV1 INFECTION IN CAPTIVE AND WILD FLORIDA MANATEES (TRICHECHUS MANATUS LATIROSTRIS)

Donà

Rehtanz

Adimey

et al. 2011

Journal of Wildlife Diseases

Self Cite

View full text Add to dashboard Cite

ABSTRACT:In 1997, cutaneous papillomatosis caused by Florida manatee (Trichechus manatus latirostris [Tm]) papillomavirus 1 (TmPV1) was detected in seven captive manatees at the Homosassa Springs Wildlife State Park, Florida, USA, and, subsequently, in two wild manatees from the adjacent Homosassa River. Since then, papillomatosis has been reported in captive manatees housed in other locations, but not in wild animals. To determine TmPV1 antibody prevalence in captive and wild manatees sampled at various locations throughout Florida coastal regions, virus-like particles, composed of the L1 capsid protein of TmPV1, were generated with a baculovirus expression system and used to measure anti-TmPV1 antibodies in an enzyme-linked immunosorbent assay. Serologic analysis of 156 manatees revealed a TmPV1 antibody prevalence of 26.3%, with no significant difference between captive (n539) and wild (n5117) manatees (28.2% and 25.6%, respectively). No antibody-positive wild animal showed PV-induced cutaneous lesions, whereas papillomatosis was observed in 72.7% of antibody-positive captive manatees. Our data indicate that Florida manatees living in the wild are naturally infected by TmPV1 but rarely show TmPV1-induced papillomatosis. Hence, it appears that the wild population would not be harmed in a case of contact with captive animals without visible lesions and productive infections, which could be thus released into the wild.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Tmpv1 L1 Cloning Expression and Detectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

SEROEPIDEMIOLOGY OF TmPV1 INFECTION IN CAPTIVE AND WILD FLORIDA MANATEES (TRICHECHUS MANATUS LATIROSTRIS)

Donà

Rehtanz

Adimey

et al. 2011

Journal of Wildlife Diseases

Self Cite

View full text Add to dashboard Cite

show abstract

“…Estas espécies estão sujeitas a diversos fatores que ameaçam as suas populações, dentre estes, o tráfego de embarcações (Kraus 1990, Wells & Scott 1997, mortalidade acidental em redes de pesca (Parente et al 2004), atividades petrolíferas (Parente 2005), degradação e contaminação dos ambientes costeiros e de águas interiores (Rosas & Lehti 1996), caça intencional (Lima 1997, Luna 2001) e a ocorrência de diversos agentes etiológicos, tais como vírus (Rector et al 2004), bactérias (Vergara- Parente et al 2003) e protozoários (Dubey et al 2003). Entre as infecções ocasionadas por protozoários, a criptosporidiose vem sendo considerada como uma doença emergente, oportunista (Xiao et al 1998) e de distribuição cosmopolita (Current 1983), além de apresentar um caráter zoonótico .…”

Section: Introductionunclassified

Criptosporidiose: uma revisão sobre a sua implicação na conservação dos mamíferos aquáticos

2007

View full text Add to dashboard Cite

Cryptosporidiosis is an emerging parasitic disease that has been related in several mammal species, including the aquatic ones. This review covers the epidemiology infection by Cryptosporidium spp. in aquatic mammals. The age of the animal, infecting dose and immunological state can influence on the clinical presentation, and when associated with other pathogen, can lead the animal to death. The water supply contamination is water supply is one of the major ways of the pathogen dissemination, which was being identified by different techniques of laboratorial analysis. The adequacy of sanitation structures, the use of appropriate method to inactivate the oocysts and the assurance of personal hygiene norms are some of the recommended way to minimize the dissemination of Cryptosporidium among aquatic mammals.Keywords: aquatic animals, epidemiology, zoonosis, Cryptosporidium. A criptosporidiose é uma doença parasitária emergente que vem sendo relatada em diversas espécies de mamíferos, incluindo os aquáticos. Esta revisão trata da epidemiologia da infecção por Cryptosporidium spp. em mamíferos aquáticos. A idade dos animais, dose infectante e o estado imunológico podem influenciar na apresentação clínica, e quando associados a outros patógenos pode levar o animal a óbito. A contaminação dos recursos hídricos constitui um dos principais meios de disseminação do parasito, que foi identificado por diferentes técnicas de análise. A adequação das estruturas de saneamento, utilização de métodos apropriados para a inativação dos oocistos e garantia das normas de higiene pessoal constituem algumas das maneiras recomendadas para minimizar a disseminação do Cryptosporidium entre os mamíferos aquáticos.Palavras-chave: animais aquáticos, epidemiologia, zoonose, Cryptosporidium.

show abstract

Nucleic Acids as Detection Tools

Lam

Aguirre

2010

The Chemical Biology of Nucleic Acids

View full text Add to dashboard Cite

In this chapter, we review some of the popular detection methods that utilize nucleic acids. First, we discuss assorted techniques in which nucleic acid probes are used to detect other nucleic acid sequences, followed by a discussion of functional nucleic acids and their applications as biosensors in conjunction with fluorescent, colorimetric, electrochemical and piezoelectric detection platforms. Detection of nucleic acid targets by nucleic acid probesSince many human diseases are linked to genetic perturbations, identification of specific mutations in patients has become a highly important practice in modern medicine. A great deal of DNA-based diagnostics pertain to identifying subtle differences in sequence content and their associated phenotypes. These differences are often the result of single nucleotide polymorphisms (SNPs) or other sequence alterations (such as point mutations, insertions and deletions). Since SNP is the most frequent form of genetic variation among individuals (roughly one for every 1000 nucleotides), many nucleic acid-based detection technologies are directed at identifying single-nucleotide differences [7,8].Apart from local genetic mutations, structural and distant site changes of DNA sequence are also observed. These large genomic alterations include insertion-deletions, gene copy number variants (CNVs), inversions and translocations, which have been linked to a variety of genetic disorders [9]. For instance, CNVs are characterized by atypical gene content and/or gene deregulation associated with CHARGE syndrome and Parkinson's and Alzheimer's disease [8,10,11]. Currently, there are a plethora of methods for detecting local and structural genetic changes, but here we will only discuss a few major techniques.

show abstract

Characterization of a Novel Close-to-Root Papillomavirus from a Florida Manatee by Using Multiply Primed Rolling-Circle Amplification: Trichechus manatus latirostris Papillomavirus Type 1

Cited by 76 publications

References 22 publications

SEROEPIDEMIOLOGY OF TmPV1 INFECTION IN CAPTIVE AND WILD FLORIDA MANATEES (TRICHECHUS MANATUS LATIROSTRIS)

SEROEPIDEMIOLOGY OF TmPV1 INFECTION IN CAPTIVE AND WILD FLORIDA MANATEES (TRICHECHUS MANATUS LATIROSTRIS)

Criptosporidiose: uma revisão sobre a sua implicação na conservação dos mamíferos aquáticos

Nucleic Acids as Detection Tools

Contact Info

Product

Resources

About