Characterization of a novel marine aerobic denitrifier Vibrio spp. AD2 for efficient nitrate reduction without nitrite accumulation

Ma, Hongjing; Liu, Ying; Ruan, Yunjie; Wei, Chenzheng; Song, Jing; Wu, Yaopan; Han, Rui

doi:10.1007/s11356-021-12673-8

Cited by 26 publications

(6 citation statements)

References 35 publications

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“…The dominant aerobic denitrifiers in our study differed from those in recirculating aquaculture systems (Deng et al, 2020a,b). Notably, commonly isolated aerobic denitrifiers from aquaculture environments, such as Bacillus (Gao et al, 2018), Marinobacter (Liu et al, 2016), Vibrio (Ren et al, 2021), and Pseudomonas (Deng et al, 2014;Hong et al, 2020;Wang et al, 2020;, were not dominant in our samples. This finding suggests that the prevailing aerobic denitrifying bacteria may not hold dominance in intensive aquaculture water as previously believed.…”

Section: Bacterial Community Composition and Dynamic Patternmentioning

confidence: 71%

“…It has been suggested that Nap is insensitive to oxygen and thus could be expressed under aerobic conditions, whereas the activity of Nar might be repressed by the presence of O 2 (Reyes et al, 1996). The napA gene, encoding Nap, is often used as a functional marker to identify aerobic denitrifiers (Zhu et al, 2012;Huang et al, 2015;Deng et al, 2020b;Ren et al, 2021). In intensive aquaculture, dissolved oxygen levels typically need to be above 4 mg/L to meet the oxygen requirements of the cultured organisms.…”

Section: Introductionmentioning

confidence: 99%

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Homogeneous environmental selection mainly determines the denitrifying bacterial community in intensive aquaculture water

Zheng,

Yan,

Zhao

et al. 2023

Front. Microbiol.

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Nitrate reduction by napA (encodes periplasmic nitrate reductase) bacteria and nitrous oxide reduction by nosZ (encodes nitrous oxide reductase) bacteria play important roles in nitrogen cycling and removal in intensive aquaculture systems. This study investigated the diversity, dynamics, drivers, and assembly mechanisms of total bacteria as well as napA and nosZ denitrifiers in intensive shrimp aquaculture ponds over a 100-day period. Alpha diversity of the total bacterial community increased significantly over time. In contrast, the alpha diversity of napA and nosZ bacteria remained relatively stable throughout the aquaculture process. The community structure changed markedly across all groups over the culture period. Total nitrogen, phosphate, total phosphorus, and silicate were identified as significant drivers of the denitrifying bacterial communities. Network analysis revealed complex co-occurrence patterns between total, napA, and nosZ bacteria which fluctuated over time. A null model approach showed that, unlike the total community dominated by stochastic factors, napA and nosZ bacteria were primarily governed by deterministic processes. The level of determinism increased with nutrient loading, suggesting the denitrifying community can be manipulated by bioaugmentation. The dominant genus Ruegeria may be a promising candidate for introducing targeted denitrifiers into aquaculture systems to improve nitrogen removal. Overall, this study provides important ecological insights into aerobic and nitrous oxide-reducing denitrifiers in intensive aquaculture, supporting strategies to optimize microbial community structure and function.

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Section: Bacterial Community Composition and Dynamic Patternmentioning

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Homogeneous environmental selection mainly determines the denitrifying bacterial community in intensive aquaculture water

Zheng,

Yan,

Zhao

et al. 2023

Front. Microbiol.

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“…Their abundances rapidly decreased after CY1, especially Vibrionaceae, whose abundance was almost zero. Although some Vibrionaceae species are pathogenic bacteria, members of this family can participate in the water-nitrogen cycle and remove various forms of nitrogen, such as ammonia nitrogen [51] and nitrate nitrogen [52], from the water. Alteromonadaceae species contain key genes involved in the nitrogen cycle, such as amoA, napA, narG, and nirB, and are functional bacteria capable of removing toxic nitrogen compounds such as nitrate [53].…”

Section: Characteristics and Succession Of Dominant Bacteria In The B...mentioning

confidence: 99%

Bacterial Community Characteristics and Roles in Nitrogen Transformation in Industrial Farming Systems of Litopenaeus vannamei

Shan,

Du,

et al. 2024

JMSE

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Industrial farming is one of the main farming systems used for L. vannamei, and it is also the main direction of development in the future. To investigate the role of the bacterial community in nitrogen transformation in industrial L. vannamei farming systems, we studied the concentration of nitrogen compounds, the bacterial community using 16S rRNA sequencing, and nitrogen cycling genes’ abundance using absolute quantitative PCR in aquaculture water (using seawater (WD) and groundwater mixed with seawater (CY)) and analyzed the correlations between them using a person analysis. The results showed that the bacterial α-diversity index (ACE, Shannon, and Sobs) significantly changed during culture in WD but not in CY. Bacterial community species composition analysis and linear discriminant analysis effect size (LEfSe analysis) revealed that Cyanobiaceae and Microbacteriaceae were the dominant bacteria and biomarkers in WD. Vibrionaceae, Ateromonadaceae, Microbacteriaceae, Saprospiraceae, and Cryomorphaceae were the dominant bacteria and biomarkers in CY. A functional annotation of procaryotic taxa (FAPROTAX) analysis revealed that the bacterial community in WD exhibited a greater phototrophic activity at early culture stages, while at the same stage, CY exhibited strong nitrate reduction. A correlation analysis of nitrogen cycling genes and environmental factors revealed that napA, narG, ureC, amoA, and nirK were significantly correlated with the concentrations of total ammonia nitrogen (TAN), nitrite (NO2−-N), nitrate (NO3−-N), and total nitrogen (TN) in WD. In CY, amoA was positively correlated and napA and nirK were negatively correlated with TAN and TN concentrations. A correlation analysis between bacterial abundance and environmental factors revealed that Flavobacteriaceae, Saprospiraceae, Cryomorphaceae, Cyanobiaceae, Halieaceae, and Cyclobacteriaceae were significantly correlated with the concentrations of TAN and NO2−-N. The above results indicated that the bacterial community in industrial shrimp farming systems changed under different conditions, with consequent changes in the abundance of genes being involved in the nitrogen cycle and in this biogeochemical process in the water. Our study facilitates further understanding of microbes and their functions in nitrogen cycling in industrial shrimp farming systems.

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“…Some HNAD bacteria have different properties and are effective in converting nitrite to gaseous nitrogen at high DO levels, such as P. stutzeri ZF31, P. mendocina X49, and Vibrio sp. AD2 (Huang et al, 2015;Ren et al, 2021;Xie et al, 2021).…”

Section: Nitrate Nitrite and Nitrous Oxide Biotransformation Pathways...mentioning

confidence: 99%

Advances in Research Into and Applications of Heterotrophic Nitrifying and Aerobic Denitrifying Microorganisms

Song²,

Wang³

et al. 2022

Front. Environ. Sci.

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With the increasing use of animal and plant proteins, pollution due to nitrogen sources is attracting increasing attention. In particular, the amount of nitrogen-containing sewage discharged into the environment has increased significantly, causing eutrophication of water bodies and environmental degradation of water quality. Traditionally, nitrifying bacteria perform ammonia nitrification under aerobic conditions, while denitrifying bacteria perform nitrate/nitrite denitrification under anaerobic conditions. However, heterotrophic nitrifying and aerobic denitrifying microorganisms (HNADs) perform ammonia nitrification and nitrate/nitrite denitrification under the same aerobic conditions using an organic carbon source, which is a much simpler and more efficient process. In this review, the distribution and evolutionary relationships of novel HNADs strains are presented, and the influencing factors, metabolic pathways, key enzymes, and practical applications of HNADs are reviewed.

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Characterization of a novel marine aerobic denitrifier Vibrio spp. AD2 for efficient nitrate reduction without nitrite accumulation

Cited by 26 publications

References 35 publications

Homogeneous environmental selection mainly determines the denitrifying bacterial community in intensive aquaculture water

Homogeneous environmental selection mainly determines the denitrifying bacterial community in intensive aquaculture water

Bacterial Community Characteristics and Roles in Nitrogen Transformation in Industrial Farming Systems of Litopenaeus vannamei

Advances in Research Into and Applications of Heterotrophic Nitrifying and Aerobic Denitrifying Microorganisms

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