Characterization of a novel polysaccharide from Moutan Cortex and its ameliorative effect on AGEs-induced diabetic nephropathy

Lian, Yuanpei; Zhu, Maomao; Chen, Juan; Yang, Bing; Lv, Qinglin; Wang, Long; Guo, Shuchen; Tan, Xuecai; Chang, Li; Bu, Weiquan; Ding, Wenbo; Jia, Xiao‐Bin; Feng, Liang

doi:10.1016/j.ijbiomac.2021.02.062

Cited by 33 publications

(11 citation statements)

References 42 publications

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“…The generation of ROS immediately initiates a variety of signal transduction pathways, such as the inflammatory response and apoptosis, which lead to damage to cell and tissue functions and promote the occurrence and development of DN. 37 In the current study, increased DKG, GLA, MDA or MGO, GO, 4-ONE, and FA levels in plasma were observed in DN mice, but the content was significantly reduced by treatment with PL. The results demonstrated that PL can improve DN mice by regulating glucose metabolism, which is consistent with the biochemical analysis.…”

Section: Discussionsupporting

confidence: 45%

“…Hyperglycemia can promote the production and accumulation of reactive carbonyl species, thereby promoting the formation of AGEs, and AGEs are considered one of the most important pathogenic substances in DN. ,, A large number of AGEs are formed and bind to the specific site of AGEs on the cell (RAGE receptor), which leads to reactive oxygen species (ROS) and stimulates oxidative stress. The generation of ROS immediately initiates a variety of signal transduction pathways, such as the inflammatory response and apoptosis, which lead to damage to cell and tissue functions and promote the occurrence and development of DN . In the current study, increased DKG, GLA, MDA or MGO, GO, 4-ONE, and FA levels in plasma were observed in DN mice, but the content was significantly reduced by treatment with PL.…”

Section: Discussionsupporting

confidence: 41%

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Metabolic Profiling of Carbonyl Compounds for Unveiling Protective Mechanisms of Pueraria lobata against Diabetic Nephropathy by UPLC-Q-Orbitrap HRMS/MS Analysis

Zhang

Guo

Zhuang

et al. 2021

J. Agric. Food Chem.

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Carbonyl compounds play a critical role in the pathogenesis of diabetic nephropathy (DN). Pueraria lobata (PL), also known as “Kudzu”, is a widely consumed functional food or nutraceutical and has shown promise in the prevention of diabetes and complications such as DN. To explore the beneficial effects and the underlying mechanisms of PL against DN, a new strategy for in-depth metabolic profiling of carbonyl compounds in DN mice plasma by chemical derivatization combined with UPLC-Q-Orbitrap high-resolution mass spectrometry (HRMS)/MS analysis was developed for the first time. Pharmacological evaluation revealed that PL extracts containing a total of 73 identified compounds could ameliorate kidney injury and regulate abnormal glycolipid metabolism. In metabolomics analysis, 19 carbonyl compounds with significant differences were identified between DN mice and normal mice. Moreover, 12 metabolites had a tendency to return to normal levels after PL treatment. Overall, PL exerts beneficial effects on DN by regulating abnormal glycolipid metabolism and carbonyl stress, and endogenous carbonyl compounds might serve as potential biomarkers for DN.

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Section: Discussionsupporting

confidence: 45%

Section: Discussionsupporting

confidence: 41%

Metabolic Profiling of Carbonyl Compounds for Unveiling Protective Mechanisms of Pueraria lobata against Diabetic Nephropathy by UPLC-Q-Orbitrap HRMS/MS Analysis

Zhang

Guo

Zhuang

et al. 2021

J. Agric. Food Chem.

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“…HMGB2 expression in tubular cells of the retinal tissues or in human HK‐2 cells was detected by immunohistochemical (IHC) and immunofluorescent staining assays, respectively, according to the methods mentioned in the previous studies 43,44 …”

Section: Methodsmentioning

confidence: 99%

β‐Amyrin ameliorates diabetic nephropathy in mice and regulates the miR‐181b‐5p/HMGB2 axis in high glucose‐stimulated HK‐2 cells

Wang¹,

Zhang

et al. 2021

Environmental Toxicology

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Diabetic nephropathy (DN) is a diabetic complication that can cause renal failure. β‐amyrin has been identified to possess anti‐diabetic property. This study was designed to evaluate the potential role of β‐amyrin in DN and its underlying mechanism. Streptozotocin‐induced diabetic mice were used as the in vivo model, and high glucose (HG)‐stimulated human proximal tubular HK‐2 cells were utilized as the in vitro model. Renal histological changes in mice were assessed by hematoxylin–eosin and periodic acid‐Schiff staining. HK‐2 cell viability and apoptosis were detected by Cell Counting Kit‐8 assay and flow cytometry analysis, respectively. β‐amyrin was found to ameliorate kidney injury in DN mice and suppressed inflammatory response as well as apoptosis of HG‐stimulated HK‐2 cells. miR‐181‐5p expression in murine renal tissues and HK‐2 cells was detected by in situ hybridization (ISH) and fluorescence in situ hybridization (FISH). MiR‐181b‐5p, a previously identified target for diabetic kidney disease, was downregulated in renal tissues and HG stimulated HK‐2 cells, and β‐amyrin induced the upregulation of miR‐181b‐5p. Binding relationship between miR‐181b‐5p and high mobility group box 2 (HMGB2) was confirmed by luciferase reporter assay. MiR‐181b‐5p bound to 3′ untranslated region of HMGB2 to suppress its expression. As shown by immunohistochemical staining and immunofluorescence staining, HMGB2 was upregulated in the in vivo and in vitro models of DN, and β‐amyrin induced the downregulation of HMGB2. Moreover, HMGB2 overexpression neutralized the suppressive effects of miR‐181b‐5p elevation on the inflammatory response and apoptosis of HG‐treated HK‐2 cells. Overall, β‐amyrin ameliorates DN in mice and suppresses inflammatory response and apoptosis of HG‐stimulated HK‐2 cells via the miR‐181b‐5p/HMGB2 axis.

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“…As shown in Figure 3 A and Figure S6B , the signals at δ 5.13/109.84 and 5.07/110.23 in HSQC spectrum of MBAP-1 were obviously observed, indicating they might be the anomeric signals of arabinose residues because the anomeric carbon signals of arabinose residues were generally at δ 105–110. GC-MS results indicated MBAP-1 consisted of →5)-α- l -Ara f -(1→ and α- l -Ara f -(1→, and the anomeric carbon signal of α- l -Ara f -(1→ was commonly in lower field than that of →5)-α- l -Ara f -(1→ [ 19 ]. Thus, the signals at δ 5.13/109.84 and 5.07/110.23 ( Figure 3 A) were assigned to →5)-α- l -Ara f -(1→ and α- l -Ara f -(1→, and named as residues C and D, respectively.…”

Section: Resultsmentioning

confidence: 99%

Two Natural Flavonoid Substituted Polysaccharides from Tamarix chinensis: Structural Characterization and Anticomplement Activities

et al. 2022

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Two novel natural flavonoid substituted polysaccharides (MBAP-1 and MBAP-2) were obtained from Tamarix chinensis Lour. and characterized by HPGPC, methylation, ultra-high-performance liquid chromatography-ion trap tandem mass spectrometry (UPLC-IT-MSn), and NMR analysis. The results showed that MBAP-1 was a homogenous heteropolysaccharide with a backbone of 4)-β-d-Glcp-(1→ and →3,4,6)-β-d-Glcp-(1→. MBAP-2 was also a homogenous polysaccharide which possessed a backbone of →3)-α-d-Glcp-(1→, →4)-β-d-Glcp-(1→ and →3,4)-β-d-Glcp-2-OMe-(1→. Both the two polysaccharides were substituted by quercetin and exhibited anticomplement activities in vitro. However, MBAP-1 (CH50: 0.075 ± 0.004 mg/mL) was more potent than MBAP-2 (CH50: 0.249 ± 0.006 mg/mL) and its reduced product, MBAP-1R (CH50: 0.207 ± 0.008 mg/mL), indicating that multiple monosaccharides and uronic acids might contribute to the anticomplement activity of the flavonoid substituted polysaccharides of T. chinensis. Furthermore, the antioxidant activity of MBAP-1 was also more potent than that of MBAP-2. In conclusion, these two flavonoid substituted polysaccharides from T. chinensis were found to be potential oxidant and complement inhibitors.

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Characterization of a novel polysaccharide from Moutan Cortex and its ameliorative effect on AGEs-induced diabetic nephropathy

Cited by 33 publications

References 42 publications

Metabolic Profiling of Carbonyl Compounds for Unveiling Protective Mechanisms of Pueraria lobata against Diabetic Nephropathy by UPLC-Q-Orbitrap HRMS/MS Analysis

Metabolic Profiling of Carbonyl Compounds for Unveiling Protective Mechanisms of Pueraria lobata against Diabetic Nephropathy by UPLC-Q-Orbitrap HRMS/MS Analysis

β‐Amyrin ameliorates diabetic nephropathy in mice and regulates the miR‐181b‐5p/HMGB2 axis in high glucose‐stimulated HK‐2 cells

Two Natural Flavonoid Substituted Polysaccharides from Tamarix chinensis: Structural Characterization and Anticomplement Activities

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