Characterization of a Novel Potyvirus Isolated from Maize in Israel

Seifers, D. L.; Salomon, R.; Marie-Jeanne, Véronique; Alliot, B.; Signoret, P. A.; Haber, Stephen; Лобода, А. В.; Ens, Werner; She, Yi‐Min; Standing, Kenneth G.

doi:10.1094/phyto.2000.90.5.505

Cited by 69 publications

(40 citation statements)

References 41 publications

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“…[26 -28], foxtail mosaic virus (FoMV) [29,30], four strains of wheat streak mosaic virus (WSMV) [31], four strains of Johnsongrass mosaic virus (JGMV) from Australia, Kansas, Nigeria, and Israel [32][33][34], and high plains virus (HPV) from Kansas and Idaho [35]. We have previously described isolation and purification of the coat proteins from these viruses by ultracentrifugal precipitation through multiple sucrose density gradients.…”

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Formation of (b_n−1 + H₂O) ions by collisional activation of maldi-formed peptide [M + H]⁺ ions in a QqTOF mass spectrometer

She

Krokhin

Spicer

et al. 2007

J. Am. Soc. Mass Spectrom.

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Collisional activation of [M ϩ H] ϩ parent ions from peptides of n amino acid residues may yield a rearrangement that involves loss of the C-terminal amino acid residue to produce (b nϪ1 ϩ H 2 O) daughters. We have studied this reaction by a retrospective examination of the m/z spectra of two collections of data. The first set comprised 398 peptides from coat protein digests of a number of plant viruses by various enzymes, where conditions in the tryptic digests were chosen so as to produce many missed cleavages. In this case, a large effect was observed-323 (b nϪ1 ϩ H 2 O) daughter ions (ϳ81%), including 185 (ϳ46%) "strong" decays with ratios (b nϪ1 ϩ H 2 O)/ (b nϪ1 ) Ͼ 1. The second set comprised 1200 peptides, all from tryptic digests, which were carried out under more stringent conditions, resulting in relatively few missed cleavages. Even here, 190 (b nϪ1 ϩ H 2 O) ions (ϳ16%) were observed, including 87 (Ͼ 7%) "strong" decays, so the effect is still appreciable. The results suggest that the tendency for (b nϪ1 ϩ H 2 O) ion formation is promoted by the protonated side chain of a non-C-terminal basic amino acid residue, in the order arginine ӷ lysine Ն histidine, and that its (non-C-terminal) position is not critical. The results can be interpreted by a mechanism in which hydrogen bonding between the protonated side chain and the (n Ϫ 1) carbonyl oxygen facilitates loss of the C-terminal amino acid residue to give a product ion having a carboxyl group at the new

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confidence: 99%

Formation of (b_n−1 + H₂O) ions by collisional activation of maldi-formed peptide [M + H]⁺ ions in a QqTOF mass spectrometer

She

Krokhin

Spicer

et al. 2007

J. Am. Soc. Mass Spectrom.

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“…A Kansas isolate of MDMV (formerly MDMV-A) was used in the present study (16). The Ne-braska source of SCMV-MDB (formerly MDMV-B) was obtained from Stan Jensen (University of Nebraska, Lincoln), and the ZeMV isolate was the original used in 2000 to describe the virus (18). Three isolates of JGMV were used: a Kansas isolate (JGMV-KS) (17); the Nigerian isolate of JGMV (JGMV-N), which was used to describe the virus (17); and the Australian isolate (JGMVAus, obtained from Dennis Persley, Indooroopilly, Australia) that infects sorghum with the Krish gene for resistance (11,15 ).…”

Section: Methodsmentioning

confidence: 99%

“…JGMV has also been documented to naturally infect sorghum in the United States (14,17). A few instances of natural infection of sorghum with SrMV in Texas have been reported (9), and ZeMV occurs in Israel, where it infects maize and sorghum (18). …”

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New Sources of Resistance in Sorghum (Sorghum bicolor) Germplasm Are Effective Against a Diverse Array of Potyvirus spp.

2012

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Seifers, D. L., Perumal, R., and Little, C. R. 2012. New sources of resistance in sorghum (Sorghum bicolor) germplasm are effective against a diverse array of Potyvirus spp. Plant Dis. 96:1775Dis. 96: -1779 Sorghum is a host to numerous Potyvirus spp. and its germplasm encompasses a wide range of infection responses to these viruses. We determined how 183 mini-core-collection sorghum germplasm accessions responded to mechanical inoculation with Maize dwarf mosaic virus (MDMV) in growth regimes in which they were maintained at 30°C followed by 16°C for 5 days. Accessions that appeared immune to MDMV in this initial screening were evaluated for their response in a similar temperature maintenance regime to mechanical inoculation with MDMV, Sugarcane mosaic virus strain MDB (SCMV-MDB), Sorghum mosaic virus (SrMV), Zea mosaic virus (ZeMV), and Kansas, Nigerian, and Australian isolates of Johnsongrass mosaic virus (JGMV-KS, -N, and -Aus, respectively). In both experiments, MDMV systemically infected all accessions except international sorghum accession number (IS) 7679 and IS 20740. These accessions also proved resistant to MDMV, SCMV-MDB, SrMV, and JGMV-N but both were susceptible to the JGMV-KS and JGMV-Aus isolates. IS 7679 but not IS 20740 was resistant to infection with ZeMV. These observations suggest that IS 7679 and IS 20740 might serve as new sources of resistance to several Potyvirus spp. that systemically infect sorghum. (ZeMV). Of these, MDMV and SCMV-MDB are most often associated with the disease called maize dwarf mosaic of corn and sorghum (21,22). JGMV has also been documented to naturally infect sorghum in the United States (14,17). A few instances of natural infection of sorghum with SrMV in Texas have been reported (9), and ZeMV occurs in Israel, where it infects maize and sorghum (18). Sorghum (Sorghum bicolor (L.) Moench) is a host to several virus species of the family Potyviridae, including Maize dwarf mosaic virus (MDMV) (formerly MDMV-A), Sugarcane mosaic virus strain MDB (SCMV-MDV; formerly MDMV-B), Johnsongrass mosaic virus (JGMV), Sorghum mosaic virus (SrMV), and Zea mosaic virusMaize dwarf mosaic of sorghum has been recognized as an important disease of sorghum in the United States (22). Two principal vectors of MDMV and SCMV-MDB are the corn leaf aphid, Rhopalosiphum maidis (Fitch), and the greenbug, Schizaphis graminum Rond. (4,7). At warm temperatures, sorghum plants respond initially to infection with mosaic symptoms but produce a red-leaf symptom when they are exposed to cooler temperatures of about 16°C (12,21). This symptom is a consequence of the necrosis induced by infection. The term "red-leaf" is also applied to sorghum genotypes that produce tan pigments during necrosis (10). In earlier literature, the mosaic reaction was described as a "resistant" response (8), although it demonstrated susceptibility to systemic virus infection. Other authors have described this "mosaic-only" response to infection at different temperatures as resistance, "tolerance", or as a reaction f...

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“…Mundialmente, o mosaico pode ser causado por cinco espécies distintas de Potyvirus que infectam o milho e constituem um subgrupo viral: SCMV, espécie que dá nome ao subgrupo; Maize dwarf mosaic virus (MDMV); Johnsongrass mosaic virus (JGMV); Sorghum mosaic virus (SrMV) (6,7,14) e o Zea mosaic virus (ZeMV), espécie recentemente sugerida como um novo membro do subgrupo (16). No Brasil, apenas as espécies SCMV e MDMV são descritas infectando naturalmente o milho.…”

Section: Palavras-chave Adicionais: Potyvirus Marafivirus Rhopalosiunclassified

Infecção mista pelo Sugarcane mosaic virus e Maize rayado fino virus provoca danos na cultura do milho no estado de São Paulo

Gonçalves¹,

Maia

Galleti³

et al. 2007

Summa phytopathol.

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(13,15), cuja incidência é comum no Brasil (17). Os sintomas iniciais de infecção em milho consistem em pontos cloróticos com disposição linear no meio, ou mais comumente, na base das folhas, que evoluem para áreas alongadas, formando um mosaico típico, o qual pode aumentar de severidade ao decorrer da infecção. O crescimento das plantas pode ser acentuadamente reduzido, conforme a espécie e estirpe do vírus e a cultivar de milho, principalmente quando a infecção ocorre nos estágios iniciais de desenvolvimento. Ocasionalmente, em cultivares mais suscetíveis, pode ocorrer o encurtamento dos entrenós. Atualmente, a doença não ocasiona grandes danos à cultura devido ao uso de híbridos e variedades resistentes ou tolerantes. Todavia, freqüentemente specific antisera detected the presence of SCMV and MRFV in these plants. SCMV infection was also confirmed by RT-PCR with specific primers and sequence analysis. In TEM observations of negatively stained preparations, flexuous (ca.770 nm) and isometric particles (ca.30 nm) were detected. In ultrathin sections, cytoplasmic inclusions, typical of Potyviridae, were observed; neither presence of spiroplasms nor phytoplasms could be noted. These results show that mixed infection by SCMV and MRFV might be responsible for the crop losses.

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Characterization of a Novel Potyvirus Isolated from Maize in Israel

Cited by 69 publications

References 41 publications

Formation of (b_n−1 + H₂O) ions by collisional activation of maldi-formed peptide [M + H]⁺ ions in a QqTOF mass spectrometer

Formation of (b_n−1 + H₂O) ions by collisional activation of maldi-formed peptide [M + H]⁺ ions in a QqTOF mass spectrometer

New Sources of Resistance in Sorghum (Sorghum bicolor) Germplasm Are Effective Against a Diverse Array of Potyvirus spp.

Infecção mista pelo Sugarcane mosaic virus e Maize rayado fino virus provoca danos na cultura do milho no estado de São Paulo

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Characterization of a Novel Potyvirus Isolated from Maize in Israel

Cited by 69 publications

References 41 publications

Formation of (bn−1 + H2O) ions by collisional activation of maldi-formed peptide [M + H]+ ions in a QqTOF mass spectrometer

Formation of (bn−1 + H2O) ions by collisional activation of maldi-formed peptide [M + H]+ ions in a QqTOF mass spectrometer

New Sources of Resistance in Sorghum (Sorghum bicolor) Germplasm Are Effective Against a Diverse Array of Potyvirus spp.

Infecção mista pelo Sugarcane mosaic virus e Maize rayado fino virus provoca danos na cultura do milho no estado de São Paulo

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Formation of (b_n−1 + H₂O) ions by collisional activation of maldi-formed peptide [M + H]⁺ ions in a QqTOF mass spectrometer

Formation of (b_n−1 + H₂O) ions by collisional activation of maldi-formed peptide [M + H]⁺ ions in a QqTOF mass spectrometer