Characterization of a Phage Resistance Plasmid, pLKS, of Silage-Making<i>Lactobacillus plantarum</i>NGRI0101

Eguchi, Tetsuo; Doi, Kunio; Nishiyama, Kanako; Ohmomo, Sadahiro; Ogata, Seiya

doi:10.1271/bbb.64.751

Cited by 27 publications

(10 citation statements)

References 21 publications

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“…Furthermore, it was found that phage resistance of Lb. plantarum NGRI 0101 could be coded on a 2 Kb plasmid pLKS 3 . The phenotype of phage resistance will be introduced to phage-sensitive strains.…”

Section: (2) Electroporation Methodsmentioning

confidence: 99%

“…A plasmid, pLKS (2.0 Kb), of Lb. plantarum NGRI 0101 isolated from silage was found to protect against phage infection, hence the mechanism of phage tolerance in silage LAB was elucidated 3 . Strains to which the phage resistance plasmid had been introduced could be used as silage inoculants, especially when grasses with a high moisture content are used.…”

Section: (5) Phage Tolerancementioning

confidence: 99%

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Silage and Microbial Performance, Old Story but New Problems

Ohmomo

Tanaka

Kitamoto

et al. 2002

JARQ

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It is generally recognized that inoculants (lactic acid bacteria: LAB) can be used as silage additives to improve the fermentation quality and to enhance animal performance. Especially in Japan, silagemaking is a major technique to produce stored feeds and the amount of silage produced in recent years has accounted for about 70% of the amount of roughages produced in farms. Nevertheless in the Japanese environment, commercial inoculants are not always suitable for silage-making. In the present paper, at first, basic aspects of silage-making will be described in Sections 1, 2 and 3, and the problems associated with silage-making for cattle in Japan will be indicated in Sections 4 and 5. For the screening of LAB strains to develop inoculants suitable for silage-making in Japan, the construction of a model system for silage fermentation involving a solid mixed culture consisting of LAB, clostridia and coliform bacteria, referred to as "pouch method", will be presented in Section 6, and the results of screening of LAB by using the pouch method in Japan and in Thailand will be introduced in Section 7. In Section 8, attempts to improve LAB strains isolated through screening by the cell fusion method and electroporation method will be discussed. Finally, aerobic spoilage of silage which is another major problem will be outlined in Section 9. Discipline: Animal industry

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Section: (2) Electroporation Methodsmentioning

confidence: 99%

Section: (5) Phage Tolerancementioning

confidence: 99%

Silage and Microbial Performance, Old Story but New Problems

Ohmomo

Tanaka

Kitamoto

et al. 2002

JARQ

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“…Lactobacillus plantarum species often harbor several plasmids (49). Several of these have been sequenced (6,11,12,20,31,40,52,58). Although most of them are of unknown function, one plasmid encoding phage resistance (pMD5057, 10,877 bp) and another plasmid (pLKS, 2,025 bp) probably introduced from another source and coding for tetracycline resistance have been described previously (12,20).…”

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confidence: 99%

“…Several of these have been sequenced (6,11,12,20,31,40,52,58). Although most of them are of unknown function, one plasmid encoding phage resistance (pMD5057, 10,877 bp) and another plasmid (pLKS, 2,025 bp) probably introduced from another source and coding for tetracycline resistance have been described previously (12,20). All are smaller than 11 kb and are predicted to replicate via the rolling-circle replication mechanism, except for the largest plasmid, pMD5057, which is predicted to replicate via the theta mechanism (12).…”

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Functional Analysis of Three Plasmids from Lactobacillus plantarum

Kranenburg

Golić

Bongers

et al. 2005

Appl Environ Microbiol

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Lactobacillus plantarum WCFS1 harbors three plasmids, pWCFS101, pWCFS102, and pWCFS103, with sizes of 1,917, 2,365, and 36,069 bp, respectively. The two smaller plasmids are of unknown function and contain replication genes that are likely to function via the rolling-circle replication mechanism. The host range of the pWCFS101 replicon includes Lactobacillus species and Lactococcus lactis, while that of the pWCFS102 replicon also includes Carnobacterium maltaromaticum and Bacillus subtilis. The larger plasmid is predicted to replicate via the theta-type mechanism. The host range of its replicon seems restricted to L. plantarum. Cloning vectors were constructed based on the replicons of all three plasmids. Plasmid pWCFS103 was demonstrated to be a conjugative plasmid, as it could be transferred to L. plantarum NC8. It confers arsenate and arsenite resistance, which can be used as selective markers.Lactic acid bacteria are used for the preservation of food and feed raw materials like milk, meat, and vegetables or other plant materials. Certain strains of lactic acid bacteria, in particular, strains from the genus Lactobacillus, have been attributed probiotic activities in humans and animals (30). Several lactic acid bacteria, including lactococci, streptococci, lactobacilli, and pediococci, are known to harbor plasmids. These may encode important traits like resistance to phages or antibiotics, lactose catabolism, and production of proteolytic enzymes or bacteriocins. Lactobacillus plantarum species often harbor several plasmids (49). Several of these have been sequenced (6,11,12,20,31,40,52,58). Although most of them are of unknown function, one plasmid encoding phage resistance (pMD5057, 10,877 bp) and another plasmid (pLKS, 2,025 bp) probably introduced from another source and coding for tetracycline resistance have been described previously (12,20). All are smaller than 11 kb and are predicted to replicate via the rolling-circle replication mechanism, except for the largest plasmid, pMD5057, which is predicted to replicate via the theta mechanism (12).The capacity for conjugal transfer is an important characteristic for plasmids. Self-transmissible conjugative plasmids have the ability to form effective cell-to-cell contact, while mobilizable plasmids are only able to prepare their DNA for transfer (38). Mobilization involves the action of a specific DNA-protein structure called the relaxosome to produce single-stranded cleavage at the nicking site (nic) within the origin of transfer (oriT) of the plasmid (38). To date, there is very little information on conjugation in lactobacilli. Sasaki et al. demonstrated conjugational transfer of the promiscuous thetareplicating plasmid pAM␤1 from Streptococcus faecalis to L. plantarum (51). Ahn et al. described an 8.5-kb chloramphenicol resistance plasmid which had been comobilized with pAM␤1 from L. plantarum to Carnobacterium maltaromaticum (3), previously known as Carnobacterium piscicola (43). To our knowledge, no conjugative L. plantarum plasmids have been reported...

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“…A similar sequence is also present in the self-transmissible conjugative lactococcal plasmids pRS01 (38) and pCI528 (33) and in several lactococcal plasmids, including pAH33 (41), pNZ4000 (48), pSRQ900 (16), pBL1 (34), and pWV01 (three copies present; 30). Plasmids from other lactic acid bacteria, such as the Lactobacillus plantarum phage resistance plasmid pLKS (15), the Lactobacillus helveticus cryptic plasmid pLH2 (43), and the Leuconostoc mesenteroides plasmid pTXL1 (accession no. AJ272077), also contain a similar element.…”

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Exploitation of Plasmid pMRC01 To Direct Transfer of Mobilizable Plasmids into Commercial Lactococcal Starter Strains

Hickey

Twomey

Ross

et al. 2001

Appl Environ Microbiol

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Genetic analysis of the 60.2-kb lactococcal plasmid pMRC01 revealed a 19.6-kb region which includes putative genes for conjugal transfer of the plasmid and a sequence resembling an origin of transfer (oriT). This oriT-like sequence was amplified and cloned on a 312-bp segment into pCI372, allowing the resultant plasmid, pRH001, to be mobilized at a frequency of 3.4 ؋ 10 ؊4 transconjugants/donor cell from an MG1363 (recA mutant) host containing pMRC01. All of the resultant chloramphenicol-resistant transconjugants contained both pRH001 and genetic determinants responsible for bacteriocin production and immunity of pMRC01. This result is expected, given that transconjugants lacking the lacticin 3147 immunity determinants (on pMRC01) would be killed by bacteriocin produced by the donor cells. Indeed, incorporation of proteinase K in the mating mixture resulted in the isolation of transformants, of which 47% were bacteriocin deficient. Using such an approach, the oriT-containing fragment was exploited to mobilize pRH001 alone to a number of lactococcal hosts. These results demonstrate that oriT of pMRC01 has the potential to be used in the development of mobilizable food-grade vectors for the genetic enhancement of lactococcal starter strains, some of which may be difficult to transform.

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Characterization of a Phage Resistance Plasmid, pLKS, of Silage-MakingLactobacillus plantarumNGRI0101

Cited by 27 publications

References 21 publications

Silage and Microbial Performance, Old Story but New Problems

Silage and Microbial Performance, Old Story but New Problems

Functional Analysis of Three Plasmids from Lactobacillus plantarum

Exploitation of Plasmid pMRC01 To Direct Transfer of Mobilizable Plasmids into Commercial Lactococcal Starter Strains

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