Characterization of a Potyvirus That Causes Zucchini Yellow Mosaic

Vaccari, Lisa; Boccardo, G.; D’Agostino, Giovanni; Dellavalle, G.; d'Aquilio, M.

doi:10.1094/phyto-71-667

Cited by 210 publications

(129 citation statements)

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“…PPV-NAT was propagated in Nicotiana clevelandii. The virus was purified according to Lisa et al (1981), and the RNA was extracted as described previously (Maiss et al, 1988).…”

Section: Methodsmentioning

confidence: 99%

The Complete Nucleotide Sequence of Plum Pox Virus RNA

Maiß¹,

Timpe²,

Brisske³

et al. 1989

Journal of General Virology

209

143

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SUMMARYThe complete nucleotide sequence of the RNA of an aphid non-transmissible plum pox virus (PPV-NAT) isolate has been determined from five overlapping cDNA clones, cDNA prepared by primer extension was used to determine the 5' terminus. The assembled RNA is 9741 nucleotides in length, excluding a 3' terminal poly(A) sequence. One large open reading frame starts at nucleotide positions 36 to 38 and is terminated with an UAG codon at positions 9522 to 9524. The putative start codon is located at positions 147 to 149. The encoded polyprotein has a predicted Mr of 353"8K. Comparison of cistrons from tobacco vein mottling virus and tobacco etch virus with those predicted for PPV-NAT indicated a similar genome organization. A highly conserved sequence of 12 nucleotides was found in the 5' non-coding region of these three potyviruses. The potential polyadenylation signal from yeast (UAUGU) was found in the 3' non-coding region of PPV-NAT and several other members of the potyvirus group.

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“…PPV-NAT was propagated in Nicotiana clevelandii. The virus was purified according to Lisa et al (1981), and the RNA was extracted as described previously (Maiss et al, 1988).…”

Section: Methodsmentioning

confidence: 99%

The Complete Nucleotide Sequence of Plum Pox Virus RNA

Maiß¹,

Timpe²,

Brisske³

et al. 1989

Journal of General Virology

209

143

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“…In 1973, a severe viral disease was observed in zucchini plants in Northern Italy (Lisa et al, 1981). The symptoms were different from those caused by the known cucurbit-infecting viruses CMV, WMV2 and PRSV-W.…”

Section: Discoverymentioning

confidence: 99%

Zucchini yellow mosaic virus

Desbiez¹,

Lecoq²

1997

Plant Pathology

224

172

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Zucchini yellow mosaic potyvirus (ZYMV), first isolated in Italy in 1973, described in 1981, and then identified in all continents within a decade, is one of the most economically important viruses of cucurbit crops. It is efficiently aphid-transmitted in a nonpersistent manner and it is also seed-borne in zucchini squash, which could have contributed to its rapid spread worldwide. Biological variability has been observed among ZYMV isolates, concerning host range, symptomatology and aphid transmissibility. More recent studies also revealed a serological and molecular variability. The survival of ZYMV in areas where cucurbits are not grown throughout the year remains to be elucidated, because very few natural over-wintering hosts have been identified so far. Partial control of ZYMV can be achieved by limiting transmission of the virus to the crops by aphids, using adapted cultural practices. Cross-protection with a mild strain has been shown to be effective against most ZYMV isolates. Resistance genes found in cucurbit germplasms are currently being introduced into cultivars with good agronomical characteristics. Pathogen-derived resistance strategies using the expression of ZYMV genes in transgenic plants have also been developed and appear promising. Nevertheless, the high biological variability of ZYMV justifies a careful evaluation of the deployment of genetic control strategies in order to increase their durability.

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“…Zucchini yellow mosaic virus (ZYMV), a member of the potyvirus group, is an important pathogen of cucurbit crops (Lisa et al, 1981). Three isolates of ZYMV differing in aphid transmissibility have previously been characterized (Antignus et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

A zucchini yellow mosaic virus coat protein gene mutation restores aphid transmissibility but has no effect on multiplication

Gal‐On

Antignus

Rosner

et al. 1992

Journal of General Virology

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An aphid-transmissible (AT) and two non-aphidtransmissible (NAT) isolates of zucchini yellow mosaic virus (ZYMV) were studied. The predicted amino acid sequences of the coat protein (CP) of the three virus isolates were analysed and compared. The NAT isolates differed from the AT isolate in having a Thr instead of an Ala residue at position 10 in the conserved Asp-Ala-Gly triplet in the N-terminal region of CP.Aphid transmissibility was restored in a progeny virus derived from an infectious clone of the ZYMV-NAT isolate in which Thr was changed back to Ala by sitedirected mutagenesis. However this mutation did not have any effect on the multiplication rate in squash, which was significantly higher than that of the AT isolate. The involvement of this mutation in aphid transmission and virus multiplication is discussed.

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Characterization of a Potyvirus That Causes Zucchini Yellow Mosaic

Cited by 210 publications

References 0 publications

The Complete Nucleotide Sequence of Plum Pox Virus RNA

The Complete Nucleotide Sequence of Plum Pox Virus RNA

Zucchini yellow mosaic virus

A zucchini yellow mosaic virus coat protein gene mutation restores aphid transmissibility but has no effect on multiplication

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