Characterization of a robust anti-Prelog short-chain dehydrogenase/reductase ChKRED20 from Chryseobacterium sp. CA49

Reconstruction of the Catalytic Pocket and Enzyme–Substrate Interactions To Enhance the Catalytic Efficiency of a Short‐Chain Dehydrogenase/Reductase

“…( S )‐2,2,2‐Trifluoro‐1‐phenylethanol is used in the synthesis of several compounds for the treatment of diseases caused by activation of microglia and has, thus, attracted intense attention from the pharmaceutical industry. Therefore, 2,2,2‐trifluoroacetophenone ( 1 h ) was selected as a model substrate to test the potential of applying the EbSDR8 variant in the bioreduction of prochiral ketones.…”

Cloning and characterization of a NADH-dependent aldo-keto reductase from a newly isolated Kluyveromyces lactis XP1461

“…It was found that catalytic activity decreased in the following order 4a >5a >3a, 12a >13a >11a, indicating steric hindrance and electronic absorption effect possibly playing important roles in enzyme-substrate affinity. The electron withdrawing groups have a positive impact on the hydrogen bond formation between enzyme active site and substrate [5].…”

“…In practical application, chiral alcohols are mainly prepared through asymmetric reduction of carbonyl compounds by transitional metal catalyst [5]. Unfortunately, this chemical strategy often suffered from high cost, poor safety together with heavy metal pollution, which restricted its large-scale application.…”

“…LB medium (pH 7.0) contains 10.0 g tryptone, 5.0 g yeast extract and 10.0 g NaCl in 1 l of distilled water. For cell growth on plates, bean sprout agar and LB agar with 1.5% (w/v) agar were used.…”

Efficient bioreductive production of (S)-N-Boc-3-hydroxypiperidine using ketoreductase ChKRED03

“…CA49 [21]. This inventory of recombinant KREDs has been applied in the bioreduction of a spectrum of chiral alcohols with excellent stereoselectivity [22][23][24][25]. We first evaluated the ability of those KREDs to catalyze the bioreduction of N-Boc-piperidin-3-one to afford (S)-NBHP (Fig.…”