Microorganisms
are essential for the formation of biogenic coalbed
methane (CBM) and the application of microbially enhanced CBM (MECoM).
Anthracite-degrading methanogenic microflora was cultivated by enrichment
and subculture from produced water obtained in Qinshui Basin where
CBM is explored commercially. The maximum methane yield was 255 μmol/g
of coal after 23 days of cultivation at 35 °C, 1.1–1.2%
of salinity, <0.15 mm of coal particle size, pH 8–9, and
5 mL of inocula/g of coal. Microflora archaea, as revealed by MiSeq,
were mainly composed of Methanosaeta, an acetic acid-consuming
methanogen, followed by Methanocella, a hydrogen-consuming
methanogen, suggesting a diversity of methanogenic pathways with a
dominance of acetoclastic methanogenesis. The bacteria mainly included Enterobacter, Acetoanaerobium, Macellibacteroides, Clostridium, and Ercella. FTIR analysis showed that the oxygen-containing
groups and aliphatic groups were the main targets for microbial degradation.
XRD analysis indicated that crystal nucleus structures of coal decreased
after degradation. The concentrations of aliphatics were shown to
increase over the incubation period based on GC–MS analysis,
while the concentrations of dissolved aromatics decreased. These results
show the potential for enhancing CBM production by stimulating the
activity of methanogenic consortia in situ in Qinshui Basin as well
as other high-rank coal reservoirs.