Characterization of a urea optical sensor based on polypyrrole

Marcos, Susana de; Hortigüela, Rubén; Gatbán, Javier; Castillo, Juan R.; Wolfbeis, Otto S.

doi:10.1007/bf01242915

Cited by 23 publications

(8 citation statements)

References 7 publications

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“…Ammonia and carbon dioxide are formed upon enzymatic hydrolysis of urea in the presence of urease. This was utilized in numerous biosensors for urea based on optical pH transduction. ,,,− Such biosensors can, in principle, also be used for analysis of metal ions due to their inhibitory action on the activity of urease . Ammonia and CO 2 also are formed on hydrolysis of creatinine in the presence of creatinine deiminase which was used for detection of this analyte with help of an optical pH transducer .…”

Section: Selected Applications Of Optical Ph Sensorsmentioning

confidence: 99%

Optical Sensing and Imaging of pH Values: Spectroscopies, Materials, and Applications

2020

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This is the first comprehensive review on methods and materials for use in optical sensing of pH values and on applications of such sensors. The Review starts with an introduction that contains subsections on the definition of the pH value, a brief look back on optical methods for sensing of pH, on the effects of ionic strength on pH values and p K a values, on the selectivity, sensitivity, precision, dynamic ranges, and temperature dependence of such sensors. Commonly used optical sensing schemes are covered in a next main chapter, with subsections on methods based on absorptiometry, reflectometry, luminescence, refractive index, surface plasmon resonance, photonic crystals, turbidity, mechanical displacement, interferometry, and solvatochromism. This is followed by sections on absorptiometric and luminescent molecular probes for use pH in sensors. Further large sections cover polymeric hosts and supports, and methods for immobilization of indicator dyes. Further and more specific sections summarize the state of the art in materials with dual functionality (indicator and host), nanomaterials, sensors based on upconversion and 2-photon absorption, multiparameter sensors, imaging, and sensors for extreme pH values. A chapter on the many sensing formats has subsections on planar, fiber optic, evanescent wave, refractive index, surface plasmon resonance and holography based sensor designs, and on distributed sensing. Another section summarizes selected applications in areas, such as medicine, biology, oceanography, bioprocess monitoring, corrosion studies, on the use of pH sensors as transducers in biosensors and chemical sensors, and their integration into flow-injection analyzers, microfluidic devices, and lab-on-a-chip systems. An extra section is devoted to current challenges, with subsections on challenges of general nature and those of specific nature. A concluding section gives an outlook on potential future trends and perspectives.

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Section: Selected Applications Of Optical Ph Sensorsmentioning

confidence: 99%

Optical Sensing and Imaging of pH Values: Spectroscopies, Materials, and Applications

2020

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“…Polypyrrole itself acts as a pH sensitive indicator in the near-IR range, with the change in urea concentration at the linear range of 0.06-1 M urea [73].…”

Section: Urea Biosensors Based On Conducting Polymer Matricesmentioning

confidence: 99%

“…Among them, polypyrrole is one of the most extensively used conducting polymers in the fabrication of urea biosensors [73][74][75]. The versatility of this polymer is determined by (i) its biocompatibility, (ii) capability to transduce energy arising from the interaction of analytes and analyte recognizing sites into electrical signals that are easily monitored, (iii) capability to protect electrodes from interfering material, and (iv) easy ways for electro-chemical deposition on the surface of any type of electrode.…”

Section: Urea Biosensors Based On Conducting Polymer Matricesmentioning

confidence: 99%

Recent developments in urea biosensors

Dhawan

Sumana

Malhotra

2009

Biochemical Engineering Journal

194

116

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“…De Marcos et al [59] developed a new optical sensor for determination of urea, on the basis of its enzymatic reaction with urease, which was photoimmobilized with polyacrylamide on a polypyrrole (PPy) film. The main advantage of this sensor is that no pH indicator dye is needed, because PPy itself acts as support and as indicator.…”

Section: Enzyme-based Biosensorsmentioning

confidence: 99%

Fiber-optic biosensors – an overview

2002

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This article reviews progress and developments during the past five years in the field of optical fiber biosensors. Because of the expense and time constraints associated with modern laboratory analysis, there is a growing need for real-time, low-cost technology that can be used industrially, environmentally, and clinically, and to monitor food processing. Miniaturization, integrated systems, and multianalyte determination have become key aspects of sensor development and efforts in this direction will also be discussed, with some pointers to likely directions of future research in the area. The review will provide information about the analytical characteristics and applications of fiber-optic biosensors classified depending on the biorecognition element employed - enzymes, whole cells, antibodies, nucleic acids, and biomimetic polymers.

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Characterization of a urea optical sensor based on polypyrrole

Cited by 23 publications

References 7 publications

Optical Sensing and Imaging of pH Values: Spectroscopies, Materials, and Applications

Optical Sensing and Imaging of pH Values: Spectroscopies, Materials, and Applications

Recent developments in urea biosensors

Fiber-optic biosensors – an overview

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