Characterization of a ZmSERK gene and its relationship to somatic embryogenesis in a maize culture

Zhang, Suzhi; Liu, Xinggui; Lin, Yueai; Xie, Guangning; Fu, Feng; Liu, Hailan; Wang, Jing; Gao, Shibin; Long, Hai; Tang, Rong

doi:10.1007/s11240-010-9834-1

Cited by 54 publications

(38 citation statements)

References 27 publications

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“…The role of SERK in induction of somatic embryogenesis has been demonstrated in many plants, both monocots and dicots (Mahdavi-Darvari et al 2014). Zhang et al (2011) has reported expression of SERK in maize was closely related to auxin signaling during initial stages of somatic embryogenesis. The expression of SERK has been reported to be reduced in a gradual manner during subsequent development stages of somatic embryogenesis (Hecht et al 2001;Schmidt et al 1997), a trend which was observed in the present study too.…”

Section: Discussionmentioning

confidence: 99%

De novo assembly and characterization of global transcriptome of coconut palm (Cocos nucifera L.) embryogenic calli using Illumina paired-end sequencing

et al. 2015

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Production and supply of quality planting material is significant to coconut cultivation but is one of the major constraints in coconut productivity. Rapid multiplication of coconut through in vitro techniques, therefore, is of paramount importance. Although somatic embryogenesis in coconut is a promising technique that will allow for the mass production of high quality palms, coconut is highly recalcitrant to in vitro culture. In order to overcome the bottlenecks in coconut somatic embryogenesis and to develop a repeatable protocol, it is imperative to understand, identify, and characterize molecular events involved in coconut somatic embryogenesis pathway. Transcriptome analysis (RNA-Seq) of coconut embryogenic calli, derived from plumular explants of West Coast Tall cultivar, was undertaken on an Illumina HiSeq 2000 platform. After de novo transcriptome assembly and functional annotation, we have obtained 40,367 transcripts which showed significant BLASTx matches with similarity greater than 40 % and E value of ≤10(-5). Fourteen genes known to be involved in somatic embryogenesis were identified. Quantitative real-time PCR (qRT-PCR) analyses of these 14 genes were carried in six developmental stages. The result showed that CLV was upregulated in the initial stage of callogenesis. Transcripts GLP, GST, PKL, WUS, and WRKY were expressed more in somatic embryo stage. The expression of SERK, MAPK, AP2, SAUR, ECP, AGP, LEA, and ANT were higher in the embryogenic callus stage compared to initial culture and somatic embryo stages. This study provides the first insights into the gene expression patterns during somatic embryogenesis in coconut.

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Section: Discussionmentioning

confidence: 99%

De novo assembly and characterization of global transcriptome of coconut palm (Cocos nucifera L.) embryogenic calli using Illumina paired-end sequencing

et al. 2015

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“…Embryo gene expression patterns may reflect changes in culture media composition and assist in improvement of in vitro protocols. Screening of specific genes may also be useful to identify those that play a prominent role in controlling embryo development (Cairney and Pullman 2007;Zhang et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Gene expression during early somatic embryogenesis in Brazilian pine (Araucaria angustifolia (Bert) O. Ktze)

Schlögl

Santos

Vieira

et al. 2011

Plant Cell Tiss Organ Cult

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Brazilian pine (Araucaria angustifolia (Bert) O. Ktze) is the only native conifer species with economic importance in Brazil. Recently, due to intensive exploitation Brazilian pine was included in the official list of endangered Brazilian plants, under the ''vulnerable'' category. Biotechnology tools like somatic embryogenesis (SE) are potentially useful for mass clonal propagation and ex situ conservation strategies of commercial and endangered plant species. In spite of that, numerous obstacles still hamper the full application of SE technology for a wider range of species, including Brazilian pine. To enhance somatic embryogenesis in Brazilian pine and to gain a better understanding of the molecular events associated with somatic embryo development, we analyzed the steady-state transcript levels of genes known to regulate somatic embryogenesis using semiquantitative reverse transcription polymerase chain reaction (sqRT-PCR). These genes included Argonaute (AaAGO), Cup-shaped cotyledon1 (AaCUC), wushel-related WOX (AaWOX), a S-locus lectin protein kinase (AaLecK), Scarecrow-like (AaSCR), Vicilin 7S (AaVIC), Leafy Cotyledon 1 (AaLEC), and a Reversible glycosylated polypeptide (AaRGP). Expression patterns of these selected genes were investigated in embryogenic cultures undergoing different stages of embryogenesis, and all the way to maturation. Up-regulation of AaAGO, AaCUC, AaWOX, AaLecK, and AaVIC was observed during transition of somatic embryos from stage I to stage II. During the maintenance phase of somatic embryogenesis, expression of AaAGO and AaSCR, but not AaRPG and AaLEC genes was influenced by presence/absence of plant growth regulators, both auxins and cytokinins. The results presented here provide new insights on the molecular mechanisms responsible for somatic embryo formation, and how selected genes may be used as molecular markers for Brazilian pine embryogenesis.

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“…Experimental systems for somatic embryogenesis are well established in some species (Raval and Chattoo 1993;Luo and Koop 1997;Nishiwaki et al 2000).Considerable efforts have been made during the past two decades to isolate and characterize genes involved in somatic embryogenesis (Lin et al 1996;Schmidt et al 1997;Zuo et al 2002;Aquea and Arce-Johnson 2008;Kiselev et al 2009;Montero-Cortes et al 2010;Zakizadeh et al 2010;Zhang et al 2011). Several genes encoding transcription factors have been isolated and identified in somatic embryogenesis.…”

Section: Introductionmentioning

confidence: 99%

Characterization and expression pattern analysis of DcNAC gene in somatic embryos of Dendrobium candidum Wall Ex Lindl.

Zhao

Wang

Sun

2011

Plant Cell Tiss Organ Cult

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The plant-specific ''no apical meristem'' genes are transcription factors that play diverse roles in plant development and stress responses. However, whether the gene family is also involved in somatic embryogenesis remains unknown, and no NAC family genes have been identified from orchid species. Here, we cloned and characterized a new member of NAC family from somatic embryos of Dendrobium candidum. Phylogenetic analysis indicated that DcNAC fell into the NAM subgroup based on similarities in protein sequence. Expression pattern analysis indicated that DcNAC exhibited a specific expression pattern in tissues examined, high levels of DcNAC mRNA was detected in protocorm-like bodies, and predominantly in the shoot apical meristem region as detected by in situ hybrization. Subcellular localization analysis showed that DcNAC protein is restricted in the nucleus through transient expression in Nicotiana benthamiana. Thus, DcNAC is the first NAC family gene isolated from orchid species, and our results provide the foundation for future investigations on the molecular mechanisms of somatic embryogenesis and for genetic improvement of Dendrobium candidum.

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Characterization of a ZmSERK gene and its relationship to somatic embryogenesis in a maize culture

Cited by 54 publications

References 27 publications

De novo assembly and characterization of global transcriptome of coconut palm (Cocos nucifera L.) embryogenic calli using Illumina paired-end sequencing

De novo assembly and characterization of global transcriptome of coconut palm (Cocos nucifera L.) embryogenic calli using Illumina paired-end sequencing

Gene expression during early somatic embryogenesis in Brazilian pine (Araucaria angustifolia (Bert) O. Ktze)

Characterization and expression pattern analysis of DcNAC gene in somatic embryos of Dendrobium candidum Wall Ex Lindl.

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