Characterization of Actinomyces turicensis and Actinomyces radingae strains from human clinical samples

Vandamme, Peter; Falsen, Enevold; Vancanneyt, Marc; Esbroeck, Marjan Van; Merwe, D Van De; Bergmans, Anneke; Schouls, Leo M.; Sabbe, L.

doi:10.1099/00207713-48-2-503

Cited by 36 publications

(28 citation statements)

References 12 publications

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“…These appear to be real associations, as the study included all A. meyeri isolates in the ARU collection, and the sources of these are remarkably similar to those of the 16 strains reported by Cato et al (4). Conversely, A. turicensis was isolated predominantly from genital or skin-related sources, similar to those reported previously for this organism (20,25,27). This species was also isolated from bacteremias in this and other studies (20,25).…”

Section: Discussionsupporting

confidence: 65%

“…This was most notable in the reassignment to other species of 81% of isolates originally identified as A. meyeri or resembling A. meyeri. These data underline those of other studies in which isolates subsequently found to be A. turicensis were variously described on the basis of phenotypic data as resembling A. meyeri (2,10), Actinomyces (now Arcanobacterium) pyogenes (28), or Gardnerella vaginalis (25,26). Reassuringly, all of 44 isolates found to be members of genera other than Actinomyces yielded distinct ARDRA profiles, and thus none was misidentified as an Actinomyces sp.…”

Section: Discussionsupporting

confidence: 56%

“…The three ARDRA profiles obtained for members of A. turicensis may denote similar variation within this species. Indeed, the dendrogram derived from whole-cell protein patterns, published by Vandamme et al (25), indicates several distinct groups within A. turicensis. Within the A. naeslundii/A.…”

Section: Discussionmentioning

confidence: 99%

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Identification of Clinical Isolates of Actinomyces Species by Amplified 16S Ribosomal DNA Restriction Analysis

et al. 2001

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Amplified 16S ribosomal DNA (rDNA) restriction analysis (ARDRA), using enzymes HaeIII and HpaII, was applied to 176 fresh and 299 stored clinical isolates of putative Actinomyces spp. referred to the Anaerobe Reference Unit of the Public Health Laboratory Service for confirmation of identity. Results were compared with ARDRA results obtained previously for reference strains and with conventional phenotypic reactions. Identities of some strains were confirmed by analysis of partial 16S rDNA sequences. Of the 475 isolates, 331 (70%) were clearly assigned to recognized Actinomyces species, including 94 isolates assigned to six recently described species. A further 52 isolates in 12 ARDRA profiles were designated as apparently resembling recognized species, and 44 isolates, in 18 novel profiles, were confirmed as members of genera other than Actinomyces. The identities of 48 isolates in nine profiles remain uncertain, and they may represent novel species of Actinomyces. For the majority of species, phenotypic results, published reactions for the species, and ARDRA profiles concurred. However, of 113 stored isolates originally identified as A. meyeri or resembling A. meyeri by phenotypic tests, only 21 were confirmed as A. meyeri by ARDRA; 63 were reassigned as A. turicensis, 7 as other recognized species, and 22 as unidentified actinomycetes. Analyses of incidence and clinical associations of Actinomyces spp. add to the currently sparse knowledge of some recently described species.

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Section: Discussionsupporting

confidence: 65%

Section: Discussionsupporting

confidence: 56%

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Identification of Clinical Isolates of Actinomyces Species by Amplified 16S Ribosomal DNA Restriction Analysis

et al. 2001

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“…Although we found that both type strains were ␣-fucosidase positive (with Rosco tablets, 4-MU-linked substrates, and the API ZYM kit), our previous experience shows that the production of ␣-fucosidase is a variable feature of A. turicensis among clinical strains. This probably reflects the vast heterogeneity of the former Actinomyces pyogenes-like (26) and A. meyeri-like (2) organisms that are included in A. turicensis (25). The separation of A. meyeri from A. turicensis is difficult.…”

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confidence: 99%

“…Classically, A. meyeri has been described as an obligatory anaerobic organism (3). As A. turicensis grows both anerobically and aerobically (25), aerotolerance may also be a phenotypic test that can be used to discriminate between these two phenotypically close species. A. turicensis may be differentiated from Arcanobacterium bernardiae by positivity for xylose fermentation or a rapid ␤-xylosidase reaction ( Fig.…”

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confidence: 99%

Phenotypic Identification of Actinomyces and Related Species Isolated from Human Sources

et al. 2001

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Recent advancements in chemotaxonomic and molecular biology-based identification methods have clarified the taxonomy of the genus Actinomyces and have led to the recognition of several new Actinomyces and related species. Actinomyces-like gram-positive rods have increasingly been isolated from various clinical specimens. Thus, an easily accessible scheme for reliable differentiation at the species level is needed in clinical and oral microbiology laboratories, where bacterial identification is mainly based on conventional biochemical methods. In the present study we designed a two-step protocol that consists of a flowchart that describes rapid, cost-efficient tests for preliminary identification of Actinomyces and closely related species and an updated more comprehensive scheme that also uses fermentation reactions for accurate differentiation of Actinomyces and closely related species.

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Actinomycesand Related Genera

Funke¹

2010

Topley &Amp; Wilson's Microbiology and Microbial Infections

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Characterization of Actinomyces turicensis and Actinomyces radingae strains from human clinical samples

Cited by 36 publications

References 12 publications

Identification of Clinical Isolates of Actinomyces Species by Amplified 16S Ribosomal DNA Restriction Analysis

Identification of Clinical Isolates of Actinomyces Species by Amplified 16S Ribosomal DNA Restriction Analysis

Phenotypic Identification of Actinomyces and Related Species Isolated from Human Sources

Actinomycesand Related Genera

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