Characterization of actinomycetes isolated from the indoor air of the church of Saint Katherine Monastery, Egypt

Abdulla, Hesham; Morshedy, Hekmate; Dewedar, Ahmed

doi:10.1007/s10453-007-9080-0

Cited by 7 publications

(5 citation statements)

References 18 publications

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“…Although results of several studies are similar to those of our study with indoor and outdoor concentrations, respectively, in 820 healthy (or reference homes) residential buildings ranged between 68–2,307 CFU/m 3 and 400–80,000 CFU/m 3 in the USA [29] and 264–17,788 CFU/m 3 and 123–5,771 CFU/m 3 in Mexico [30]. Moreover, in Egypt, fungal concentrations were found in the range of 10–10 2 CFU/m 3 in the indoor air of the Church of Saint Katherine Monastery in Sinai [31], and 52–124 CFU/m 3 indoors and 25–222 CFU/m 3 outdoors at the coastal buildings in Domitta [32]. …”

Section: Discussionmentioning

confidence: 99%

Coarse and Fine Culturable Fungal Air Concentrations in Urban and Rural Homes in Egypt

Awad

Gibbs

Tarwater

et al. 2013

IJERPH

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The main objective of the present study was to assess culturable airborne fungal concentrations, and types in different seasons. Two-stage viable impactor samplers were used with malt extract agar medium as the collection media. Culturable airborne fungal concentrations were collected indoors and outdoors of 43 homes in urban and rural environments from November 2008 to October 2009 in Egypt. Fungal concentrations were significantly higher in the rural environment than the urban environment. The median indoor and outdoor total fungal concentrations were 608 and 675 CFU/m3 in the urban environment and 1,932 and 1,872 CFU/m3 in the rural environment, respectively. The greatest concentrations were found in the autumn and spring season. Indoor and outdoor concentrations were significantly correlated (P < 0.001). The highest concentrations were observed in the fungal size range of <8 µm (fine fraction). The indoor/outdoor (I/O) ratios were not statistically different between seasons. Alternaria, Aspergillus, Cladosporium, Penicillium and yeasts were the predominant genera indoors and outdoors, and the abundance of genera varied by season and region. This study is of a potential interest as little reported research on the indoor fungal air quality from Egypt.

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Section: Discussionmentioning

confidence: 99%

Coarse and Fine Culturable Fungal Air Concentrations in Urban and Rural Homes in Egypt

Awad

Gibbs

Tarwater

et al. 2013

IJERPH

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“…One example is the species belonging to the genus Bacillus, four of which were isolated from both pyramids and frequently identified on stone buildings (Kiel and Gaylarde, 2006). An exclusive bacterium to LP belonged to the genus Micromonospora, which was occasionally reported from decayed stone (Ciferri et al, 2000), along with isolates from Micropolyspora, and Streptomyces, which were previously reported from a tomb in Tella Baste, Zagazig city in Egypt (Abdulla et al, 2008). Other isolates were best known as common environmental species of natural presence on soil [e.g., Arthrobacter (Eschbach et al, 2003)].…”

Section: Discussionmentioning

confidence: 99%

“…Despite the predominance of isolates of the genus Streptomyces , members of the genera Geodermatophilus and Rhodococcus were also reported ( Groth et al, 1999 ). In addition to Streptomyces and Nocardia , Micromonospora species were also isolated from ancient stones from a tomb site in Tell Basta (Zagazig city, Egypt ( Abdulla et al, 2008 )).…”

Section: Introductionmentioning

confidence: 99%

Culturable and unculturable potential heterotrophic microbiological threats to the oldest pyramids of the Memphis necropolis, Egypt

et al. 2023

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A large percentage of the world’s tangible cultural heritage is made from stone; thus, it deteriorates due to physical, chemical, and/or biological factors. The current study explored the microbial community inhabiting two prehistoric sites with high cultural value in the Memphis necropolis of Egypt (Djoser and Lahun Pyramids) using amplicon-based metabarcoding and culture-dependent isolation methods. Samples were examined by epifluorescent microscopy for biological signs before environmental DNA extraction and in vitro cultivation. The metabarcoding analysis identified 644 bacterial species (452 genera) using the 16S rRNA and 204 fungal species (146 genera) using ITS. In comparison with the isolation approach, an additional 28 bacterial species (13 genera) and 34 fungal species (20 genera) were identified. A total of 19 bacterial and 16 fungal species were exclusively culture-dependent, while 92 bacterial and 122 fungal species were culture-independent. The most abundant stone-inhabiting bacteria in the current study were Blastococcus aggregatus, Blastococcus saxobsidens, and Blastococcus sp., among others. The most abundant rock-inhabiting fungi were Knufia karalitana and Pseudotaeniolina globosa, besides abundant unknown Sporormiaceae species. Based on previous reports, microorganisms associated with biodeterioration were detected on color-altered sites at both pyramids. These microorganisms are potentially dangerous as physical and chemical deterioration factors and require proper conservation plans from a microbiological perspective.

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“…5H 2 O 0.01, agar 20/L distilled water) supplemented with antibacterial chloramphenicol (0.05 g/l) to inhibit the rapid growth of bacteria, plates were incubated for 5 days at 25°C, where micro fungi appeared (Šimonovicova et al 2004). Air samples were collected from the air within the investigated tombs according to Abdulla et al (2008) using automated air sampler (Microbio Air Sampler MB2, Parrett LTD, UK) where fixed volume of air samples were collected over 1 min, directly onto Dox-Czapek agar medium plates, incubated for 5 days at 25°C.…”

Section: Figure2 Percentage Of Fungal Isolatesmentioning

confidence: 99%

Effect of Thymol against Fungi Deteriorating Mural Paintings at Tell Basta Tombs, Lower Egypt

2018

International Journal of Research Studies in Biosciences

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INTRODUCTIONMural paintings within Tell Basta (Bubastis) tombs are often subjected to fungal deterioration, since fungi play an important role in biodeterioration of mural paintings. Fungi cause chromatic alteration through forming irreversible stains resulted in secreting extracellular in nature of melanin and carotenoid pigments (Sterflinger et al. 1999), even after the fungus is dead, the pigmented cell walls remain on the surface of the paintings and stone surfaces and these strains are resistant to chemical and enzymatic degradation (Kiyuna et al. 2011). The structural damage is caused by fungal metabolites of acids that are extremely erosive (Sterflinger, 2010) and these acids cause chromatic alterations of paintings (Ravikumar et al. 2012). Furthermore, produced enzymes could decompose the binding media of the mural paintings, such as egg yolk, animal glue and arabic gum into free amino acids and mono sugars that could be used as carbon source for fungal growth and colonization (Milanesi et al. 2006). Fungal deterioration of mural paintings in ancient Egyptian tombs was put onto the evidence, since mural paintings in the tomb of the King Tutankhamen (18 th Dynasty) were stained with brown-black spots, that resulted in the growth and colonization of Aspergillus penicilloides (Arai 2004;Vasanthakumar et al. 2013). Furthermore, Cladosporium sp. disfigured mural paintings in the tomb of the King Amenophis III (18 th Dynasty) by forming black stains (Arai 2004). Also Penicillium, Aspergillus, Alternaria and Cladosporium hebarum formed black stains on mural paintings within the tomb of Petosiris that dated back to the Ptolemaic period (El-Deeb et al. 2000). Moreover, Cladosporium showed to be involved in disfiguration of stone surfaces in the tomb of the Queen Nefertari (18 th Dynasty) with black spots (Preusser 1987). In addition, Cladosporium cladosporoides, Alternaria alternata and Aspergillus niger caused discoloration of mural paintings in the Temple of Abydos (19 th Dynasty) with dark brown stains (El Sharouny et al. 2000b).

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Characterization of actinomycetes isolated from the indoor air of the church of Saint Katherine Monastery, Egypt

Cited by 7 publications

References 18 publications

Coarse and Fine Culturable Fungal Air Concentrations in Urban and Rural Homes in Egypt

Coarse and Fine Culturable Fungal Air Concentrations in Urban and Rural Homes in Egypt

Culturable and unculturable potential heterotrophic microbiological threats to the oldest pyramids of the Memphis necropolis, Egypt

Effect of Thymol against Fungi Deteriorating Mural Paintings at Tell Basta Tombs, Lower Egypt

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