Characterization of Adenocarcinoma’s Autofluorescence Properties Using Multiexcitation Analysis Method

Zain, Hamdani; Tedjo, Aryo; Kusmardi, Kusmardi

doi:10.7454/msk.v11i2.260

Cited by 1 publication

(2 citation statements)

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“…The optical properties of the samples were measured using a photometer ( Figure 1) as employed by Zain et al [10].. Firstly, 400 mg of dried sample powder was formed into a solid pellet. Before the sample analysis, the intensity of the photometer was adjusted using a standard white color.…”

Section: Measurement Of the Optical Properties Of The Samplesmentioning

confidence: 99%

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A Simple Photometer and Chemometrics Analysis for Quality Control of Sambiloto (Andrographis paniculata) Raw Material

Heryanto¹,

Permana²,

Tedjo³

et al. 2017

J. Pure App. Chem. Res.

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In this paper, we described the use of a light emitting diode (LED)-based photometer and chemometric analysis for quality control of king of bitter or sambiloto (Andrographis paniculata) raw material. The quality of medicinal plants was determined by their chemical composition. The quantities of chemical components in medicinal plants can be assessed using spectroscopic technique. We used an in-house photometer to generate spectra of sambiloto. The spectra were analyzed by chemometric methods, i.e. principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA), with the aim of herbal quality classification based on the harvesting time. From the results obtained, based on thin layer chromatography analysis, sambiloto with different collection times (1, 2, and 3 months) contained different amounts of active compounds. Evaluation of sambiloto, using its spectra and chemometric analysis has successfully differentiated its quality based on harvesting time. PCA with the first two PC's (PC-1 = 60% and PC-2 = 35%) was able to differentiate according to the harvesting time of sambiloto. Three models were obtained by PLS-DA and could be used to predict unknown sample of sambiloto according to the harvesting time.

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Section: Measurement Of the Optical Properties Of The Samplesmentioning

confidence: 99%

“…The photometer utilizes light emitting diodes (LED) as radiation sources, light dependent resistors (LDR) as transducers, and a voltmeter as readout and display. This photometer has been used for classification of cancerous and healthy skin tissue [10].…”

Section: Introductionmentioning

confidence: 99%