Characterization of Alkaline Phosphatase Producing Bacteria Isolated from Thai Fermented Fish Products

Abstract

Seventeen isolates of alkaline phosphatase (ALP) producing bacteria were screened and systematically studied. They were divided into 10 groups on the basis of their phenotypic characteristics and 16S rRNA gene sequence analyses. The Gram-positive coccal isolates in Group I (3 isolates), Group II (3 isolates) and Group III (1 isolate) showed 99.2%, 99.5-99.7% and 99.8% sequences similarity to Staphylococcus saprophyticus, S. napalensis and S. sciuri, respectively. Each Gram-positive rod-shaped isolates in Group IV and V belonged to the genus Bacillus and was closely related to B. vietnamensis and B. safensis with 99.2% and 99.4% sequences similarity, respectively. The Gram-positive, moderately halophilic rod-shaped bacteria in Group VI (3 isolates), Group VII (1 isolate), Group VIII (1 isolate) and Group IX (2 isolates) were closely related to Virgibacillus halodenitrificans (98.5-99.1%), Oceanobacillus iheyensis (99.3%), Halobacillus mangrove (97.9%) and H. dabanensis (98.5-98.6%), respectively. One Gram-negative isolate of moderately halophilic bacterium (Group X) was closely related to Idiomarina zobelli (98.0%). They possessed phosphatase activities ranged from 10.08-70.96 U ml-1 . The isolate NSW 13-2 (Group VI) showed the highest ALP.

Molecular and biochemical characterization of a new endoinulinase producing bacterial strain of Bacillus safensis AS-08

“…are well established. Similar biochemical characteristics of Bacillus safensis have been reported earlier (Satomi et al 2006;Raja & Omine 2012;Sitdhipol et al 2012) which corroborates our results. The bacterial isolate AS-08 showed growth at pH range of 6.0-9.0 and temperature range of 25-42 • C. The strain was found to be salt resistant as it grew well in the presence of high concentration of NaCl (0.5-12.0%).…”

“…Bacillus safensis has been reported as a potent bacterial source of alkaline phosphatase (Sitdhipol et al 2012). Raja & Omine (2012) have reported arsenic, boron and salt resistant strain of Bacillus safensis, which can be useful in remediation of salt and metal polluted soils.…”

Assessment of Phosphorus Mobilizing Capacity of Bacillus spp. Isolated from Mangrove Rhizospheric Sediment and its Potential Application in Aquaculture

“…Phosphatase producing bacteria (PPB) are the heterotrophic bacteria that produce extracellular enzymes like alkaline phosphatase that can mineralize organic phosphates into inorganic form, which is then available to plants (George et al, 2002) or exchanged into water as soluble P. Alkaline phosphatase is a hydrolyse enzyme that is responsible for removing P from 5' and 3' phosphate groups from many types of molecules including DNA, RNA, proteins etc. and produce inorganic phosphate (Sitdhipol et al, 2012). Several studies reports that bacterial species of Bacillus, Pseudomonas, Vibrio, Azobacter, Aereobacter, Rhizobium, Agrobacterium, Enterococcus, Microccocus, Achromobacter, Flavobacterium, Paenibacillus exhibit phosphatase activity (Behera et al, 2017;Olenska et al, 2020;Audipudi et al, 2012;Kathiresan and Selvam 2006;Sreevidya and Gopalakrishnan 2017;Chandran et al, 2021).…”

A method for the transient inhibition of toxicity of secretory recombinant proteins, exemplified by bacterial alkaline phosphatase. Novel protocol for problematic DNA termini dephosphorylation

“…Alkaline phosphatases (ALPs) (EC 3.1.3.1) are commonly found in nature: in bacteria, shrimp, human tissues or calf intestines [ 1 , 2 ]. ALPs are useful molecular tools [2] and are widely used for: enzyme immunoassays, clinical diagnosis, milk industry, cosmetics, phosphatase-mediated bioremediation of toxic heavy metals, as therapeutic drugs for LPS-mediated diseases and as biofertilizer [3] , [4] , [5] , [6] . The major function of E. coli BAP is to provide inorganic phosphate when the environment is deprived of this compound [7] .…”