Characterization of an alkylated dinucleotide by desorption chemical ionization and tandem mass spectrometry

Abstract

A highly sensitive (subpicomole level), structure-specific method of analysis has been developed for characterizing and quantitating the dinucleotide thymidylyl(3'----5')thymidine methyl phosphotriester by desorption chemical ionization tandem mass spectrometry. The methodology can be applied to related compounds such as the parent dinucleotide and employs either positive or negative ionization mass spectra or daughter spectra. The procedure allows specification of the site of methyl attachment.

Mass spectrometry for the analysis of carcinogen‐DNA adducts

“…An important variation on this general method has been described by Cooks and co-workers (42,43). They advocate the importance of tandem mass spectrometry as well as the use of isotope dilution for quantification.…”

“…For example, methyl dThd dimers have been studied with DCI MS (125) and LD FTMS (126). Both techniques yielded the appropriate ions to identify the phosphate group as the site of modification.…”

Tandem Mass Spectrometry of Small, Multiply Charged Oligonucleotides

“…We are aware of no MS/MS data published for multiply charged oligonucleotide anions, although preliminary experiments have been briefly described [20]. In fact, relatively few MS/MS data have been reported for singly charged oligonucleotides of "~2 [8,[24][25][26][27][28][29][30][31]. We have therefore studied the behavior of several multiply charged oligonucleotides from " = 4-8 with a quadrupole ion trap to determine their behavior under ion trap collisional activation conditions.…”

Recent Developments in Mass Spectrometry for the Characterization of Nucleosides, Nucleotides, Oligonucleotides, and Nucleic Acids

“…A multitude of reports have been published concerning the use of tandem mass spectrometry to unravel complete DNA sequencing information. These tandem mass spectrometric methods used several different kinetic MS/MS instruments which involved metastable kinetic ion decomposition (MIKE) 47 as well as low-energy 48 and high-energy collision-induced dissociation (CID) analyses of the deprotonated DNA anions, including sector instruments, and Fourier transform ion cyclotron mass spectrometry. − …”