Characterization of an ecto-5′-nucleotidase (EC 3.1.3.5) activity in intact trophozoites of Trichomonas gallinae

Characterization of an ecto-5′-nucleotidase activity present on the cell surface of Tritrichomonas foetus

“…3). The apparent K m value of T. foetus ecto-5 nucleotidase for 5 AMP is very similar with K m values found for T. vaginalis ecto-5 nucleotidase (Tasca et al, 2003) and Trichomonas gallinae ecto-5 nucleotidase (Borges et al, 2007), although the maximal capacity is lower. To verify the substrate specificity of T. foetus ecto-5 -nucleotidase activity, other 5 -nucleoside monophosphates were tested.…”

“…Ecto-5 -nucleotidases have been described in some protozoan parasites, such as G. duodenalis (Russo-Abrahão et al, 2011), T. vaginalis (Tasca et al, 2003) and T. gallinae (Borges et al, 2007). Little is known about ecto-5 -nucleotidase in T. foetus.…”

E-NTPDases and ecto-5′-nucleotidase expression profile in rat heart left ventricle and the extracellular nucleotide hydrolysis by their nerve terminal endings

“…1C), we tested the alkaline phosphatase classical inhibitors, levamisole and tetramisole (Borges et al, 2007) to discard its participation in the AMPase activity. As seen in the Table 2, these inhibitors did not decrease the AMPase activity.…”

Partial Purification and Properties of Adenosine Triphosphatase (ATPase) from Liver Fluke Fasciola hepatica

“…The adenosine triphosphatase (ATP phosphohydrolase, EC 3.6.1.3.; ATPase) is a membrane -bound enzyme which transport protons across the plasma membrane using ATP as an energy source (1)(2)(3)(4). It also plays a crucial role in oxidative phosphorylation upon the formation of ATP in biological membrane containing respiratory apparatus.…”