Characterization of an encapsulation device for the production of monodisperse alginate beads for cell immobilization

Serp, D.; Cantana, E.; Heinzen, Christoph; Stockar, Urs von; Marison, I. W.

doi:10.1002/1097-0290(20001005)70:1<41::aid-bit6>3.0.co;2-u

Cited by 175 publications

(66 citation statements)

References 54 publications

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“…The initial cell densities in these microcapsules are in the optimal range of 1.5 – 2.0×10 6 cells/cm 3 . This range of maximum initial cell density is comparable to that achieved with the conventional droplet generation method [13]. …”

Section: Resultssupporting

confidence: 55%

“…Electrostatic droplet generation is the most widely used method in the production of microcapsules containing islets or other insulin-secreting cells [12]. Typically, a laminar liquid jet is broken into droplets by a harmonically vibrating nozzle combined with an electrostatic dispersion mechanism which prevents droplet aggregation [13]. Currently, droplet generator encapsulation systems are commercially available from several manufacturers such as Inotech Biosystem (Rockville, MD) and Nisco Engineering AG (Zürich, Switzerland).…”

Section: Introductionmentioning

confidence: 99%

“…Currently, droplet generator encapsulation systems are commercially available from several manufacturers such as Inotech Biosystem (Rockville, MD) and Nisco Engineering AG (Zürich, Switzerland). The electrostatic droplet generator system is appropriate for the continuous production of hydrogel microcapsules (200–600 µm in diameter) from a polymer/cell mixture of an unchanged composition [13]. However, this apparatus, which requires sterilization of the bioreactor chamber after each use, is not convenient for studies which involve screening a large number of different material formulations [14].…”

Section: Introductionmentioning

confidence: 99%

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Microfabrication of homogenous, asymmetric cell-laden hydrogel capsules

Dang

Bratlie

et al. 2009

Biomaterials

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Cell encapsulation has been broadly investigated as a technology to provide immunoprotection for transplanted endocrine cells. Here we develop new fabrication methods that allow for rapid, homogenous microencapsulation of insulin-secreting cells with varying microscale geometries and asymmetrically modified surfaces. Micromolding systems were developed using polypropylene mesh, and the material/surface properties associated with efficient encapsulation were identified. Cells encapsulated using these methods maintain desirable viability and preserve their ability to proliferate and secrete insulin in a glucose-responsive manner. This new cell encapsulation approach enables a practical route to an inexpensive and convenient process for the generation of cell-laden microcapsules without requiring any specialized equipment or microfabrication process.

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Section: Resultssupporting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Microfabrication of homogenous, asymmetric cell-laden hydrogel capsules

Dang

Bratlie

et al. 2009

Biomaterials

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“…Despite the ease of use, alginate‐PLL capsule membranes have additional drawbacks that drive research toward more clinically and economically feasible materials . For instance, alginate‐PLL capsules have poor long‐term durability in chelating agents typically present in physiologic solutions, PLL is expensive and cytotoxic, and capsules under strain are more likely to rupture than to deform …”

Section: Introductionmentioning

confidence: 99%

Cell microencapsulation with synthetic polymers

Olabisi

2014

J Biomedical Materials Res

111

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The encapsulation of cells into polymeric microspheres or microcapsules has permitted the transplantation of cells into human and animal subjects without the need for immunosuppressants. Cell-based therapies use donor cells to provide sustained release of a therapeutic product, such as insulin, and have shown promise in treating a variety of diseases. Immunoisolation of these cells via microencapsulation is a hotly investigated field, and the preferred material of choice has been alginate, a natural polymer derived from seaweed due to its gelling conditions. Although many natural polymers tend to gel in conditions favorable to mammalian cell encapsulation, there remain challenges such as batch to batch variability and residual components from the original source that can lead to an immune response when implanted into a recipient. Synthetic materials have the potential to avoid these issues; however, historically they have required harsh polymerization conditions that are not favorable to mammalian cells. As research into microencapsulation grows, more investigators are exploring methods to microencapsulate cells into synthetic polymers. This review describes a variety of synthetic polymers used to microencapsulate cells. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 846–859, 2015.

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“…Bechhold (1907) introduced the term ultrafiltration and developed the first nitrocellulose membranes  1925: Sartorius Werke GmbH: the 1 st membrane company established to supply microporous membranes, which is viewed as the beginning of MF (Judd, 2006)  1939: Müller used MF for cultivate microbes in drinking water , the first large-scale MF application (Judd, 2006)  1947: Alwall (1947 invented the UF process for dialysis of the blood in vivo  1969: Dorr-Oliver developed the 1 st membrane bioreactors (MBRs) (Judd, 2006), and Smith et al (1969) used UF for activated sludge separation  1960s and 1970s: Forbes in 1970: first industrial applications of UF in electrophoretic painting. MF became important in biological and pharmaceutical manufacturing (Wickramasinghe, 2008)  1989: MBRs with submerged membranes in bioreactors developed (Yamamoto, 1989)  1990s: Commercialization of immersed MBRs (Judd, 2006); membrane contactors and reactors were developed (Sirkar and Prasad, 1992)  2000s: Commercialization of vertical immersed hollow-fiber MBRs (Judd, 2006)  2010: Various applications as pretreatment to NF/RO and for drinking water supply RO/NF  1949: UCLA used RO membrane for seawater desalination (Mehdizadeh, 1990).  1953: Reid presented RO systems (Uemura and Henmi, 2008)  1964: RO technologies weres on the market (Uemura and Henmi, 2008).…”

Section: Introduction and Overviewmentioning

confidence: 99%

The Values of Membrane Science and Technology: Introduction and Overview

Zhang¹,

Surampalli²,

Vigneswaran³

2012

Membrane Technology and Environmental Applications

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Nowadays, human beings are facing global challenges such as population explosion, increasing demand for food and water, resource depletion, and changing climate conditions and have been forced to move forward to development of sustainable society. Among many potential technologies, membrane technology is one of the most direct, effective and feasible approaches to solving these sophisticated problems.As a semipermeable barrier between two phases, a membrane can separate one component that moves through the membrane faster than other components of a mixture. Although the historical development of membrane science and technology can be traced back to 1748, before the 1960s, membranes were only used in laboratories or for small-scale applications in industry and life sciences. In the 1960s and 1970s, microfiltration (MF) became important in biological and pharmaceutical manufacturing. Since the 1970s, membrane technology has been used in a large scale in many different areas, especially for seawater desalination and water/wastewater treatment. During the past two decades, the energy consumption and capital/operational costs of membrane systems have declined considerably, while the availability, efficiency and reliability of new membranes and systems have increased significantly, which has resulted in worldwide exponential growth of applications of membrane technology. Nowadays, membrane technologies have emerged as the focal point of separation processes and industrial production of high quality water.There is a timely need for a book with updated, useful and systematized information on membrane technology and its environmental applications. The objectives of this book are to provide: a) comprehensive information on the principles of membrane technology and its environmental applications; and b) a general overview of recent advances in membrane technology, critical analysis of new membranes systems and applications, and future directions of development in the field of membrane technology. As an introduction of the book, this chapter covers a broader picture of membrane technology. The chapter starts with a brief introduction of the key development in the membrane technology realm, followed by an overview 1 Membrane Technology and Environmental Applications Downloaded from ascelibrary.org by University Of Auckland on 03/15/15.

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Characterization of an encapsulation device for the production of monodisperse alginate beads for cell immobilization

Cited by 175 publications

References 54 publications

Microfabrication of homogenous, asymmetric cell-laden hydrogel capsules

Microfabrication of homogenous, asymmetric cell-laden hydrogel capsules

Cell microencapsulation with synthetic polymers

The Values of Membrane Science and Technology: Introduction and Overview

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