Characterization of an Ex Vivo Model for the Assessment of Root Canal Disinfection

A New Noninvasive Model to Study the Effectiveness of Dentin Disinfection by Using Confocal Laser Scanning Microscopy

“…2A1-A4) in which no disinfecting agent was used demonstrated that only 4%-6% of the bacteria were dead, which is normal for any untreated bacterial population (3,23). It has been previously suggested that the root canal sample contents might contain autofluorescent and detrital materials that can be confused with bacteria, and background fluorescence was occasionally observed within the canal lumen (24). Nevertheless, the intensity of the background fluorescence was minimal within the tubules (12).…”

Response of E. faecalis biofilms to different associations of auxiliary substances during root canal preparation: A confocal laser microscopy analysis

“…The use of dentin to grow biofilms can result in background difficulties, because the autofluorescence of dentin potentially obscures the bacterial count when the software‐based analysis is performed (Bryce et al, 2011; Ma et al, ; Villette et al, ; Zapata et al, ). Although, Zapata et al () demonstrated that CLSM is able to show individual bacterial cells inside dentinal tubules and that the viability of bacteria in infected dentin can be determined in situ in an effective way.…”

Antibacterial Efficacy of MTAD Final Rinse and Two Percent Chlorhexidine Gel Medication in Teeth with Apical Periodontitis: A Randomized Double-blinded Clinical Trial

“…Because root canal disinfection is the main focus of endodontic treatment, it would be appropriate to develop a universal standard for assessing the antibacterial efficacy of endodontic treatment regimens. As a first step, meticulously designed in vitro, ex vivo (37), and in vivo studies are required to establish sensitive and reproducible sampling and enumeration methods. As a second step, additional microscopic methods need to be established to provide further insight into bacterial viability.…”