Response of <i>E. faecalis</i> biofilms to different associations of auxiliary substances during root canal preparation: A confocal laser microscopy analysis

Hohscheidt, Gabriela Luisa; Böttcher, Daiana Elisabeth; Parolo, Clarissa Cavalcanti Fatturi; Montagner, Francisco; Grecca, Fabiana Soares

doi:10.1002/jemt.22215

Cited by 15 publications

(12 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Following the same methodology to the present study, but using EDTA for smear layer removal, Hohscheidt et al . () found a remarkably higher percentage of bacteria reduction when compared to our results. Despite the association between NaOCl and CHX presented greater mean values for percent reduction of total bacterial load, no statistical differences were found.…”

Section: Methodsmentioning

confidence: 99%

Confocal microscopy evaluation of the effect of irrigants on Enterococcus faecalis biofilm: An in vitro study

et al. 2015

Self Cite

View full text Add to dashboard Cite

Summary: The purpose of this study was to evaluate in vitro the effectiveness of two endodontic irrigants and their association against Enterococcus faecalis (E. faecalis) by confocal laser scanning microscope (CLSM). Twentyfour bovine incisors were inoculated in a monoculture of E. faecalis for 21 days. After this period, the teeth were divided into three test groups (n ¼ 5) according to the chemical used. Group 1: 2.5% sodium hypochlorite (NaOCl), group 2: 2% chlorhexidine gel (CHX), group 3: 2.5% NaOCl þ 2% CHX gel, and two control groups (n ¼ 3): negative control group (NCG)-sterile and without root canals preparation and positive control group (PCG)-saline. Then, the samples were stained with SYTO9 and propidium iodide and subjected to analysis by CLSM. Bacterial viability was quantitatively analyzed by the proportions of dead and live bacteria in the biofilm remnants. Statistical analysis was performed by the Oneway ANOVA test (p ¼ 0.05). No statistical differences were observed to bacterial viability. According to CLSM analysis, none of the tested substances could completely eliminate E. faecalis from the root canal space. Until now, there are no irrigant solutions able to completely eliminate E. faecalis from the root canal. In this regard, the search for irrigants able to intensify the antimicrobial action is of paramount importance. SCANNING 38:57-62, 2016.

show abstract

Section: Methodsmentioning

confidence: 99%

Confocal microscopy evaluation of the effect of irrigants on Enterococcus faecalis biofilm: An in vitro study

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…Another point is the use of irrigant to complement traditional methods of cleaning and shaping 1,11,23) . The irrigant requires time to play its role, so the fastest preparation does not always mean the most effective cleaning.…”

Section: Discussionmentioning

confidence: 99%

Influence of Prior Cervical Enlargement on Apical Cleaning Using Single File

Leonardi

Schramm

Giovanini

et al. 2015

Bull. Tokyo Dent. Coll.

View full text Add to dashboard Cite

The goal of this study was to compare the apical cleaning capabilities of single files from 3 different rotary systems in the presence or absence of prior cervical preparation based on a histological analysis. A total of 84 human single-rooted mandibular permanent incisors were divided into 6 groups (14 canals each). Cleaning and shaping was performed under the following protocols: Group I, F2 ProTaper at working length; Group II, SX ProTaper and F2 ProTaper; Group III, size 25, .06 taper Mtwo at working length; Group IV, SX ProTaper and size 25, .06 taper Mtwo; Group V, size 25, .06 taper BR3 BioRace at working length; and Group VI, SX ProTaper and size 25, .06 taper BR3 BioRace. After cleaning and shaping, the root canals were evaluated by histological analysis. The percentage of remaining debris was evaluated using a cross-hatched grid superimposed over each image. Data were assessed using the Shapiro-Wilk and ANOVA tests. Statistically significant differences were observed between groups (p=0.0001) with respect to amount of remaining debris; use of SX in conjunction with F2 ProTaper yielded a significantly lower mean percentage of debris. It was concluded that cleaning ability improves when root canal preparation with F2 ProTaper is complemented by prior cervical enlargement.

show abstract

“…As previously described (22), the SYTO9 probe (excitation at 488 nm and emission at 525 nm) labels all bacteria in a population, whereas the propidium iodide probe (excitation at 488 nm and emission at 560 nm) penetrates only bacteria with a damaged plasmatic membrane. Thus, bacteria incubated in the presence of the 2 markers simultaneously fluoresces green in color if live and red if dead (23). The dye was applied over the dentin specimen for 5 minutes at a 1:1 ratio (total volume of 20 mL).…”

Section: Confocal Laser Scanning Analysismentioning

confidence: 99%

Evaluation of the Effect of Enterococcus faecalis Biofilm on the 2% Chlorhexidine Substantivity: An In Vitro Study

Böttcher

Sehnem

Montagner

et al. 2015

Journal of Endodontics

Self Cite

View full text Add to dashboard Cite

Response of E. faecalis biofilms to different associations of auxiliary substances during root canal preparation: A confocal laser microscopy analysis

Abstract: None of the tested substances could completely eliminate Ef from the root canal space.

Cited by 15 publications

References 23 publications

Confocal microscopy evaluation of the effect of irrigants on Enterococcus faecalis biofilm: An in vitro study

Confocal microscopy evaluation of the effect of irrigants on Enterococcus faecalis biofilm: An in vitro study

Influence of Prior Cervical Enlargement on Apical Cleaning Using Single File

Evaluation of the Effect of Enterococcus faecalis Biofilm on the 2% Chlorhexidine Substantivity: An In Vitro Study

Contact Info

Product

Resources

About