Evaluation of the Effect of Enterococcus faecalis Biofilm on the 2% Chlorhexidine Substantivity: An In Vitro Study

Böttcher, Daiana Elisabeth; Sehnem, Nicole Teixeira; Montagner, Francisco; Parolo, Clarissa Cavalcanti Fatturi; Grecca, Fabiana Soares

doi:10.1016/j.joen.2015.04.016

Cited by 34 publications

(27 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The period of 48 h, for which the splints were worn, was evaluated in preliminary experiments. This period enables the individually formed initial biofilm to be analyzed in a reproducible manner and has already been selected as the examination period in many other studies [3, 10, 21, 29, 44]. …”

Section: Discussionmentioning

confidence: 99%

Comparison of intraoral biofilm reduction on silver-coated and silver ion-implanted stainless steel bracket material

et al. 2018

View full text Add to dashboard Cite

PurposeThe objective of this in situ study was to quantify the intraoral biofilm reduction on bracket material as a result of different surface modifications using silver ions. In addition to galvanic silver coating and physical vapor deposition (PVD), the plasma immersion ion implantation and deposition (PIIID) procedure was investigated for the first time within an orthodontic application.Materials and methodsAn occlusal splint equipped with differently silver-modified test specimens based on stainless steel bracket material was prepared for a total of 12 periodontally healthy patients and was worn in the mouth for 48 h. The initially formed biofilm was fluorescently stained and a quantitative comparative analysis of biofilm volume, biofilm surface coverage and live/dead distribution of bacteria was performed by confocal laser scanning microscopy (CLSM).ResultsCompared to untreated stainless steel bracket material, the antibacterial effect of the PIIID silver-modified surface was just as significant with regard to reducing the biofilm volume and the surface coverage as the galvanically applied silver layer and the PVD silver coating. Regarding the live/dead distribution, however, the PIIID modification was the only surface that showed a significant increase in the proportion of dead cells compared to untreated bracket material and the galvanic coating.ConclusionsOrthodontic stainless steel with a silver-modified surface by PIIID procedure showed an effective reduction in the intraoral biofilm formation compared to untreated bracket material, in a similar manner to PVD and galvanic silver coatings applied to the surface. Additionally, the PIIID silver-modified surface has an increased bactericidal effect.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparison of intraoral biofilm reduction on silver-coated and silver ion-implanted stainless steel bracket material

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The bactericidal effect of the drug is due to the cationic molecule binding to extra microbial complexes and negatively charged microbial cell walls, thereby altering the cells’ osmotic equilibrium [30,31]. Chlorhexidine with a concentration of 2%, in both liquid or gel presentations, has been employed as an auxiliary chemical substance during root canal preparation or as a final irrigant [32,33,34]. Several in vitro studies assessed the properties of CHX as an intracanal medicament [10,35,36].…”

Section: Discussionmentioning

confidence: 99%

Synthesis and Characterization of New Chlorhexidine-Containing Nanoparticles for Root Canal Disinfection

et al. 2016

Self Cite

View full text Add to dashboard Cite

Root canal system disinfection is limited due to anatomical complexities. Better delivery systems of antimicrobial agents are needed to ensure efficient bacteria eradication. The purpose of this study was to design chlorhexidine-containing nanoparticles that could steadily release the drug. The drug chlorhexidine was encapsulated in poly(ethylene glycol)–block–poly(l-lactide) (PEG–b–PLA) to synthesize bilayer nanoparticles. The encapsulation efficiency was determined through thermogravimetric analysis (TGA), and particle characterization was performed through microscopy studies of particle morphology and size. Their antimicrobial effect was assessed over the endodontic pathogen Enterococcus faecalis. The nanoparticles ranged in size from 300–500 nm, which is considered small enough for penetration inside small dentin tubules. The nanoparticles were dispersed in a hydrogel matrix carrier system composed of 1% hydroxyethyl cellulose, and this hydrogel system was observed to have enhanced bacterial inhibition over longer periods of time. Chlorhexidine-containing nanoparticles demonstrate potential as a drug carrier for root canal procedures. Their size and rate of release may allow for sustained inhibition of bacteria in the root canal system.

show abstract

“…QMix also comprises chlorhexidine and a detergent (surfactant agent). Chlorhexidine has a broad spectrum of antimicrobial activity 23 , substantivity 24 and promotes a reduction of dentinal microhardness 25 , while the surfactant agent decreases the surface tension and increases the wettability 26 , increasing the effectiveness in penetrating deeper into the dentinal tubules, thus contributing to decontamination of root canal system. For these reasons and according to current results, the present study suggests that the QMix with US may be a good protocol for final irrigation, reinforcing the findings of Niu et al 27 , where activation of QMix appears to maximize the smear layer removal, bringing significant benefits to endodontic therapy.…”

Section: Discussionmentioning

confidence: 99%

Influence of ultrasonic activation in association with different final irrigants on intracanal smear layer removal

et al. 2016

View full text Add to dashboard Cite

Aim: To evaluate the influence of ultrasonic activation (US) with different irrigant regimens in smear layer removal. Methods: One hundred bovine incisors were instrumented and divided into ten groups (n=10) according to final irrigation protocols: distilled water (DW); DW+US; 17% EDTA; QMix; 10% citric acid; 37% phosphoric acid; 17% EDTA+US; QMix+US; 10% citric acid+US; 37% phosphoric acid+US. The samples were then submitted to scanning electron microscopy where a score system was used to evaluate the images and effectiveness of proposed treatments. The data were statistically analyzed by Kruskal-Wallis and Mann-Whitney U tests for intergroup comparisons as well as the Wilcoxon and Friedman tests for intragroup comparisons at 5% significance level. Results: In the cervical third, groups 17% EDTA, QMix, 10% citric acid, 17% EDTA+US, QMix+US and 10% citric acid+US were more effective in smear layer removal (p<0.05); in the middle third, groups 17% EDTA+US and QMix+US were more effective in smear layer removal (p<0.05); in the apical third, groups 17% EDTA,17% EDTA+US and QMix+US were more effective in smear layer removal (p<0.05). Conclusions: US can aid 17% EDTA and QMix in smear layer removal at the middle third and QMix at the apical third, contributing to the cleaning of root canal system.

show abstract

Evaluation of the Effect of Enterococcus faecalis Biofilm on the 2% Chlorhexidine Substantivity: An In Vitro Study

Cited by 34 publications

References 35 publications

Comparison of intraoral biofilm reduction on silver-coated and silver ion-implanted stainless steel bracket material

Comparison of intraoral biofilm reduction on silver-coated and silver ion-implanted stainless steel bracket material

Synthesis and Characterization of New Chlorhexidine-Containing Nanoparticles for Root Canal Disinfection

Influence of ultrasonic activation in association with different final irrigants on intracanal smear layer removal

Contact Info

Product

Resources

About