Characterization of an F18<sup>+</sup>enterotoxigenic<i>Escherichia coli</i>strain from post weaning diarrhoea of swine, and of its conjugative virulence plasmid pTC

Olasz, Ferenc; Fekete, Péter Zsolt; Blum-Oehler, Gabriele; Boldogkői, Zsolt; Nagy, Béla

doi:10.1016/j.femsle.2005.01.057

Cited by 18 publications

(24 citation statements)

References 30 publications

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“…The F18-encoding plasmid in this study, pF18, was also found to contain a hemolysin determinant. A later study involving a 120-kb plasmid known as pTC identified that it encoded STa, STb, and tetracycline resistance (134). Later analysis of pTC found that it contained a putative pathogenicity island (PAI) with a 10-kb fragment containing genes encoding the ST enterotoxins (52).…”

Section: Porcine Etec Plasmidsmentioning

confidence: 99%

Pathogenomics of the Virulence Plasmids ofEscherichia coli

Johnson

Nolan

2009

Microbiol Mol Biol Rev

376

369

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SUMMARY Bacterial plasmids are self-replicating, extrachromosomal elements that are key agents of change in microbial populations. They promote the dissemination of a variety of traits, including virulence, enhanced fitness, resistance to antimicrobial agents, and metabolism of rare substances. Escherichia coli, perhaps the most studied of microorganisms, has been found to possess a variety of plasmid types. Included among these are plasmids associated with virulence. Several types of E. coli virulence plasmids exist, including those essential for the virulence of enterotoxigenic E. coli, enteroinvasive E. coli, enteropathogenic E. coli, enterohemorrhagic E. coli, enteroaggregative E. coli, and extraintestinal pathogenic E. coli. Despite their diversity, these plasmids belong to a few plasmid backbones that present themselves in a conserved and syntenic manner. Thanks to some recent research, including sequence analysis of several representative plasmid genomes and molecular pathogenesis studies, the evolution of these virulence plasmids and the implications of their acquisition by E. coli are now better understood and appreciated. Here, work involving each of the E. coli virulence plasmid types is summarized, with the available plasmid genomic sequences for several E. coli pathotypes being compared in an effort to understand the evolution of these plasmid types and define their core and accessory components.

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Section: Porcine Etec Plasmidsmentioning

confidence: 99%

Pathogenomics of the Virulence Plasmids ofEscherichia coli

Johnson

Nolan

2009

Microbiol Mol Biol Rev

376

369

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“…The presence of various toxins in ETEC can be explained by the presence of conjugative plasmids widely distributed among porcine ETEC strains. The ST genes, sta and stb, and a tetracycline resistance gene were located on a self conjugative 120-kb plasmid, called pTC (19).…”

Section: Discussionmentioning

confidence: 99%

Frequency of virulence genes in Escherichia coli strains isolated from piglets with diarrhea in the North Parana State, Brazil

Vidotto¹,

Lima²,

Fritzen³

et al. 2009

Braz. J. Microbiol.

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Identification of Escherichia coli causing porcine postweaning diarrhea requires knowledge regarding the prevalent pathotypes within a given region. A total of 100 Escherichia coli isolates from piglets with diarrhea in Londrina city, Parana State, South Brazil, were screened for the presence of genes for F4, F5, F6, F18, F41 fimbrial antigens by specific probes and for enterotoxins (STa, STb, LT and STx2e) by polymerase chain reaction (PCR). The results showed that 60% of the isolates were positive for one or more of the fimbrial antigens and 92% were positive at least for one of the virulence factors examined. Virulence factor genes detected were F4 (44%), F18 (38%), F5 (30%), F41 (32%), F6 (25%), LTp-I (71%), STa (40%), STb (47%) and STx2e (3%). Twenty four patterns of virulence factor according to the different virulence genes form were found and the most frequent virulence gene pattern was F4, F18, F41, STa, STb and LT. Most of the isolates that carried genes for adhesins also harboured genes for toxins.

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“…Many members of the -fimbriae that are present in E. coli or S. enterica are carried by conjugative plasmids (2,3,40,96,242,301,359,360). Conjugative plasmids with similarity to the well-characterized fertility (F) plasmid are found in a considerable fraction of E. coli isolates (38).…”

Section: The -Fimbriae: Flexible Fibrillaementioning

confidence: 99%

Evolution of the Chaperone/Usher Assembly Pathway: Fimbrial Classification Goes Greek

Nuccio

Bäumler

2007

Microbiol Mol Biol Rev

298

456

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SUMMARY Many Proteobacteria use the chaperone/usher pathway to assemble proteinaceous filaments on the bacterial surface. These filaments can curl into fimbrial or nonfimbrial surface structures (e.g., a capsule or spore coat). This article reviews the phylogeny of operons belonging to the chaperone/usher assembly class to explore the utility of establishing a scheme for subdividing them into clades of phylogenetically related gene clusters. Based on usher amino acid sequence comparisons, our analysis shows that the chaperone/usher assembly class is subdivided into six major phylogenetic clades, which we have termed α-, β-, γ-, κ-, π-, and σ-fimbriae. Members of each clade share related operon structures and encode fimbrial subunits with similar protein domains. The proposed classification system offers a simple and convenient method for assigning newly discovered chaperone/usher systems to one of the six major phylogenetic groups.

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Characterization of an F18⁺enterotoxigenicEscherichia colistrain from post weaning diarrhoea of swine, and of its conjugative virulence plasmid pTC

Cited by 18 publications

References 30 publications

Pathogenomics of the Virulence Plasmids ofEscherichia coli

Pathogenomics of the Virulence Plasmids ofEscherichia coli

Frequency of virulence genes in Escherichia coli strains isolated from piglets with diarrhea in the North Parana State, Brazil

Evolution of the Chaperone/Usher Assembly Pathway: Fimbrial Classification Goes Greek

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Characterization of an F18+enterotoxigenicEscherichia colistrain from post weaning diarrhoea of swine, and of its conjugative virulence plasmid pTC

Cited by 18 publications

References 30 publications

Pathogenomics of the Virulence Plasmids ofEscherichia coli

Pathogenomics of the Virulence Plasmids ofEscherichia coli

Frequency of virulence genes in Escherichia coli strains isolated from piglets with diarrhea in the North Parana State, Brazil

Evolution of the Chaperone/Usher Assembly Pathway: Fimbrial Classification Goes Greek

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Characterization of an F18⁺enterotoxigenicEscherichia colistrain from post weaning diarrhoea of swine, and of its conjugative virulence plasmid pTC