Characterization of an Isolate of Zucchini Yellow Mosaic Virus form Cucumber in Singapore

Wong, Sek‐Man; Chng, C. G.; Chong, Pui-Chih

doi:10.1111/j.1439-0434.1994.tb04510.x

Cited by 16 publications

(9 citation statements)

References 24 publications

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“…Different working groups also reported opposing results. LECOQ and WIPF-SCHEIBEL (unpublished in LECOQ et al 2003) could not confirm any virus contamination in 70,000 seedlings from ZYMV-infected Cucurbitaceae of various cultivars (of different species) and ZYMV transmission was neither found in C. pepo (NAMETH et al 1985), Cucumis melo L. (GLEASON and PROVVI-DENTI 1990;WONG et al 1994), C. sativus, C. moschata (ROBIN-SON et al 1993) nor in C. maxima (GREBER et al 1987) seed. In contrast, DAVIS and MIZUKI (1986) found ZYMV seed transmission in seedlings of C. pepo, at a maximum rate of 80%.…”

Section: Discussionmentioning

confidence: 99%

Zucchini yellow mosaic virus — incidence and sources of virus infection in field-grown cucumbers and pumpkins in the Spreewald, Germany

et al. 2006

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In a survey in field-grown cucumber (Cucumis sativus L.) of the Spreewald cultivation area, Germany, from 2001 to 2004, infection with zucchini yellow mosaic virus (ZYMV), watermelon mosaic virus (WMV), cucumber mosaic virus (CMV) and cucumber green mottle mosaic virus (CGMMV) were recorded. ZYMV was of most relevance, owing to its damaging effects and frequency. Pumpkins (Cucurbita pepo L. and Cucurbita maxima Duchesne) were also found to be infected.In glasshouse experiments with 27 fresh and pickle cucumber cultivars frequently cultivated in the area, all cultivars proved (after mechanical inoculation) to be susceptible to ZYMV. ZYMV-infected cucumbers showed leaf chlorosis and mosaic, as well as deformation of leaves and fruits. Mechanical inoculation with ZYMV, in the early developmental stages of cucumber plants, reduced yields by up to 70%. Mixed infection with ZYMV and CMV resulted in synergistic effects, increasing the severity of damage. Fruit and leaf deformation also occurred in cucumber cultivars exhibiting a documented tolerance to CMV.The widespread weeds fat hen (Chenopodium album L.) and hoary alison (Berteroa incana (L.) DC.) were identified as possible sources of natural ZYMV infection in the Spreewald region. ZYMV transmission through seed seems to be of no importance, and possible only if fresh seeds from diseased cucumbers or pumpkins are used (commercially available seed is not contaminated). Uptake of ZYMV through contaminated soil is not a major mode of natural virus transmission, even though it proved possible in baiting experiments. Epidemiologic and economic implications of ZYMV infections of cucumber and pumpkin plants are discussed.In Untersuchungen zum Auftreten von Viren im Freilandanbau von Gurken im Spreewald wurden in den Jahren 2001 bis 2004 Zucchinigelbmosaikvirus (ZYMV), Wassermelonenmosaikvirus (WMV), Gurkenmosaikvirus (CMV) und das Grünscheckungsmosaikvirus der Gurke (CGMMV) nachgewiesen. Von besonderer Bedeutung erwies sich ZYMV im Untersuchungszeitraum durch sein häufiges Auftreten und die großen Schäden. Das Virus war sowohl in Gurken (Cucumis sativus L.) als auch in Kürbissen (Cucurbita pepo L. und Cucurbita maxima Duchesne) nachzuweisen.Versuche mittels mechanischer Inokulation ergaben für alle 27 geprüften Gurkensorten eine Anfälligkeit gegenüber ZYMV. Die Blätter ZYMV-infizierter Pflanzen zeigten Chlorosen und Mosaik, die Früchte waren deformiert. Pflanzen, die in frühen Entwicklungsstadien durch mechanische Inokulation infiziert wurden, reagierten mit Ertragsdepressionen von bis zu 70 Prozent. Bemerkenswert ist, dass eine Mischinfektion von ZYMV und CMV sichtbare Schäden an Sorten verursacht, welche als tolerant gegenüber CMV beschrieben werden.Auf der Suche nach möglichen Infektionsquellen von ZYMV in der Region Spreewald wurden 23 verschiedene Unkrautarten getestet. Natürliche Infektionen mit dem Virus wurden in Weißem Gänsefuß (Chenopodium album L.) und Graukresse (Berteroa incana (L.) DC) gefunden. Eine Übertragung von ZYMV durch kontaminiertes Saatgut wurde bis...

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Section: Discussionmentioning

confidence: 99%

Zucchini yellow mosaic virus — incidence and sources of virus infection in field-grown cucumbers and pumpkins in the Spreewald, Germany

et al. 2006

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“…Virus particles appear coated by a 'halo' of antibody molecules (Milne & Luisoni, 1977). ISEM procedures provide a high sensitivity and allow detection of viruses present in mixed infections, even at very low concentrations, as well as the establishment of serological relationships between strains or viruses (Lesemann et al, 1983;Wong et al, 1994).…”

Section: Electron Microscopymentioning

confidence: 99%

“…The virus is extracted from leaves of zucchini squash (Lisa et al, 1981), muskmelon (Lecoq & Pitrat, 1985) or pumpkin (Wong et al, 1994) plantlets 2-4 weeks after inoculation. After homogenization in phosphate buffer and low speed centrifugation, the virus is sedimented by high speed centrifugation, and further purified by sucrose density gradient (Lisa et al, 1981), or caesium sulphate gradient (Lecoq & Pitrat, 1985).…”

Section: Purificationmentioning

confidence: 99%

Zucchini yellow mosaic virus

Desbiez¹,

Lecoq²

1997

Plant Pathology

224

172

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Zucchini yellow mosaic potyvirus (ZYMV), first isolated in Italy in 1973, described in 1981, and then identified in all continents within a decade, is one of the most economically important viruses of cucurbit crops. It is efficiently aphid-transmitted in a nonpersistent manner and it is also seed-borne in zucchini squash, which could have contributed to its rapid spread worldwide. Biological variability has been observed among ZYMV isolates, concerning host range, symptomatology and aphid transmissibility. More recent studies also revealed a serological and molecular variability. The survival of ZYMV in areas where cucurbits are not grown throughout the year remains to be elucidated, because very few natural over-wintering hosts have been identified so far. Partial control of ZYMV can be achieved by limiting transmission of the virus to the crops by aphids, using adapted cultural practices. Cross-protection with a mild strain has been shown to be effective against most ZYMV isolates. Resistance genes found in cucurbit germplasms are currently being introduced into cultivars with good agronomical characteristics. Pathogen-derived resistance strategies using the expression of ZYMV genes in transgenic plants have also been developed and appear promising. Nevertheless, the high biological variability of ZYMV justifies a careful evaluation of the deployment of genetic control strategies in order to increase their durability.

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“…Sve forme i varijeteti obične tikve (C. pepo) uključene u ova ispitivanje pokazale su izuzetnu osjetljivost prema ZYMV. Između ispitivanih izolata postojala je razlika u tipu i vremenu ispoljavanja simptoma, iako su uglavnom izazvali tipove simptoma koji su ranije zabilježeni u literaturi na C. pepo (Wong et al, 1994;Prieto et al, 2001;Dukić et al, 2002;Svoboda and Polak, 2002;Tóbiás and Palkovics, 2003;Hosseini et al, 2007;Yakoubi et al, 2008;Vučurović et al 2009c).…”

Section: Biološka Identifikacija I Karakterizacija Zymvunclassified

“…Komercijalno dostupne sorte krastavaca u Srbiji bile su sistemično zaražene ZYMV što je u skladu sa rezultatima drugih autora (Wong et al, 1994;Prieto et al, 2001;Dukić et al, 2002;Tóbiás and Palkovics, 2003;Hosseini et al, 2007;Yakoubi et al, 2008;Vučurović i sar. 2009c).…”

Section: Biološka Identifikacija I Karakterizacija Zymvunclassified

Diversity, biological and molecular characterization of cucurbit viruses and disease epidemiology in Serbia

Vučurović¹

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In the period from 2007 to 2011 constant high levels of viral infections were detected in 61 cucurbit growing localities in Serbia. The frequency of viral infection fluctuated over the years, ranging from 30-50% in 2007, to the extremely high 80% in the epidemic year of 2008, while it was constant and around 40% in the next two years. In the last year of investigation, the frequency reached a very high level of 60%. Serological testing of 1100 collected samples proved the presence of three viruses: Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus (WMV) and Cucumber mosaic virus (CMV). Additional testing of symptomatic samples revealed the presence of Cucurbit aphid-borne yellows virus (CABYV), which represents the first detection of CABYV in Serbia, and Tomato spotted wilt virus (TSWV), which is the first report of TSWV on cucurbits in Serbia and the first report of Cucurbita maxima as natural host plant worldwide. ZYMV was the most prevalent virus during this five year survey. Mixed infections were the most frequent infection type in the first two years, while single infection prevailed in the last three years of investigation. During this investigation, transmission of ZYMV by seeds of C. pepo var. styriaca, C. maxima and C. moschata was proved. Both annual and perennial weeds, which are present during the establishment of cucurbit crops, and in the beginning stages of vegetation, were infected with ZYMV, WMV, CMV and TSWV. The most prevalent was TSWV which was detected in eight different weed species, the second most prevalent virus in weeds was CMV, detected in seven different weed species, while the prevalence of ZYMV and WMV was similar and they were detected in five and four weed species, respectively. A high diversity of aphids which are visiting cucurbit crops was detected through the determination of 57 aphid taxons. The most common aphid species were Aphis fabae, Anoecia corni, Aphis gossypii, Myzus persicae and Acyrthosiphon pisum. The greatest diversity and the largest number of aphids were detected in the first few weeks after plant sprouting. The detection and identification of the species level for all five cucurbit viruses in Serbia were confirmed by RT-PCR method and suitable specific primers. Bioassay on 45 test plants was used to determine phenotypic variability and host range for ZYMV, WMV and CMV. During the course of this investigation protocols for molecular detection of all five cucurbit viruses in Serbia were established or improved. Molecular characterizations of ZYMV, WMV, CMV, TSWV and CABYV were conducted by sequence and phylogenetic analyses. Sequence analysis of the CP gene revealed that Serbian ZYMV isolates belong to the Central European group of isolates. Sequence analysis based on NIb and CP genes showed that Serbian WMV isolates are grouped in two groups, and one of them represents a group of "emerging" strains containing isolates which are known for causing severe damaging symptoms on cucurbits, and this represents the first report of these isolates in Serbia. Bas...

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Characterization of an Isolate of Zucchini Yellow Mosaic Virus form Cucumber in Singapore

Cited by 16 publications

References 24 publications

Zucchini yellow mosaic virus — incidence and sources of virus infection in field-grown cucumbers and pumpkins in the Spreewald, Germany

Zucchini yellow mosaic virus — incidence and sources of virus infection in field-grown cucumbers and pumpkins in the Spreewald, Germany

Zucchini yellow mosaic virus

Diversity, biological and molecular characterization of cucurbit viruses and disease epidemiology in Serbia

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