Characterization of ancestral chromosome fusion points in the Indian muntjac deer

Hartmann, N. R.; Scherthan, Harry

doi:10.1007/s00412-003-0262-4

Cited by 54 publications

(68 citation statements)

References 33 publications

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“…These non-telomeric sites of (TTAGGG)n repeats and the colocalization of telomeric repeats and satellite DNA sequences are most likely the result of chromosome rearrangements involving the chromosome ends (including inversions, centric fusion, and telomere-to-telomere fusion) (Go et al, 2000;Li et al, 2000;Lear, 2001;Hartmann and Scherthan, 2004;Ventura et al, 2006;Tsipouri et al, 2008). In addition, sequence analysis of ITSs revealed that the interstitial arrays of (TTAGGG)n repeats could be inserted at intrachromosomal sites through double-strand breaks in ancient chromosomes (Azzalin et al, 2001;Nergadze et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Chromosome Structures between the Chicken and Three Anserid Species, the Domestic Duck (Anas platyrhynchos), Muscovy Duck (Cairina moschata), and Chinese Goose (Anser cygnoides), and the Delineation of their Karyotype Evolution by Comparative Chromosome Mapping

et al. 2014

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To better understand the process of karyotype evolution in Galloanserae (Galliformes and Anseriformes), we performed comparative chromosome painting with chicken chromosome-specific DNA probes and FISH mapping of the 18S-28S ribosomal RNA (rRNA) genes, telomeric (TTAGGG)n repeats, and cDNA clones of 37 genes for three anserid species, the domestic duck (Anas platyrhynchos), Muscovy duck (Cairina moschata), and Chinese goose (Anser cygnoides). Each chicken probe of chromosomes 1-9 and the Z chromosome painted a single pair of chromosomes in the three species except for the chromosome 4 probe, which painted acrocentric chromosome 4 and a pair of microchromosomes. The 18S-28S rRNA genes were localized to four pairs of microchromosomes in the domestic duck and Muscovy duck, and eight pairs of microchromosomes in the Chinese goose. The (TTAGGG)n repeats were localized to both telomeric ends of all chromosomes in the three species, and were additionally located in the interstitial region of the short arm of chromosome 1 in the domestic duck and in the centromeric region of chromosome 1 in the Muscovy duck. Comparative gene mapping of 37 chicken chromosome 1-4-linked and Zlinked genes revealed high chromosome homologies among three anserid species and also between the chicken and the three anserid species, although there were several chromosome rearrangements: pericentric inversion in chromosome 2, pericentric and paracentric inversions or centromere repositioning in the Z chromosome between the chicken and the anserid species, and pericentric inversions in chromosome 4 and the Z chromosome between the Chinese goose and the two duck species. These results collectively suggest that karyotypes have been highly conserved in Anseriformes and that chromosome rearrangements also occurred less frequently between Galliformes and Anseriformes after they diverged around 100 million years ago.

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Section: Discussionmentioning

confidence: 99%

Comparison of the Chromosome Structures between the Chicken and Three Anserid Species, the Domestic Duck (Anas platyrhynchos), Muscovy Duck (Cairina moschata), and Chinese Goose (Anser cygnoides), and the Delineation of their Karyotype Evolution by Comparative Chromosome Mapping

et al. 2014

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“…Large ITSs usually originate after the occurrence of chromosome rearrangements, especially Robertsonian (Rb) translocations, which result after loss of the chromosome end protection in telocentric chromosomes, allowing the fusion of two chromosomes and thus producing a neo-metacentric chromosome that may or may not retain telomeric sequences in the newly formed centromere (Garagna et al 1995;Bouffler 1998). When the telomeric repeats are maintained, they result in ITSs (Hsu et al 1975;Simons and Rumpler 1988;Meyne et al 1990;Garagna et al 1997;Hartmann and Scherthan 2004). Indeed, ITSs are mostly located at the pericentromeric regions of neometacentric chromosomes and, after the Rb fusion, they undergo amplification, allowing the stabilization of the neo-centromere through the formation of pericentromeric heterochromatin (Ruiz-Herrera et al 2008;Rovatsos et al 2011).…”

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confidence: 99%

Chromatin Organization and Remodeling of Interstitial Telomeric Sites During Meiosis in the Mongolian Gerbil (Meriones unguiculatus)

et al. 2014

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Telomeric DNA repeats are key features of chromosomes that allow the maintenance of integrity and stability in the telomeres. However, interstitial telomere sites (ITSs) can also be found along the chromosomes, especially near the centromere, where they may appear following chromosomal rearrangements like Robertsonian translocations. There is no defined role for ITSs, but they are linked to DNA damage-prone sites. We were interested in studying the structural organization of ITSs during meiosis, a kind of cell division in which programmed DNA damage events and noticeable chromatin reorganizations occur. Here we describe the presence of highly amplified ITSs in the pericentromeric region of Mongolian gerbil (Meriones unguiculatus) chromosomes. During meiosis, ITSs show a different chromatin conformation than DNA repeats at telomeres, appearing more extended and accumulating heterochromatin markers. Interestingly, ITSs also recruit the telomeric proteins RAP1 and TRF1, but in a stage-dependent manner, appearing mainly at late prophase I stages. We did not find a specific accumulation of DNA repair factors to the ITSs, such as gH2AX or RAD51 at these stages, but we could detect the presence of MLH1, a marker for reciprocal recombination. However, contrary to previous reports, we did not find a specific accumulation of crossovers at ITSs. Intriguingly, some centromeric regions of metacentric chromosomes may bind the nuclear envelope through the association to SUN1 protein, a feature usually performed by telomeres. Therefore, ITSs present a particular and dynamic chromatin configuration in meiosis, which could be involved in maintaining their genetic stability, but they additionally retain some features of distal telomeres, provided by their capability to associate to telomere-binding proteins.

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“…The most well-known hypothesis is the tandem fusion hypothesis, first proposed by Hsu et al (1975), suggesting that the 2n = 6♀/7♂ karyotype of M. muntjak vaginalis could have evolved from 2n = 46 M. reevesi-like ancestral karyotype through extensive tandem fusions and several centric fusions. Subsequent studies, including conventional comparative cytogenetics (Shi et al, 1980;Elder & Hsu, 1988;Fontana & Rubini, 1990), chromosome painting (Yang et al, 1995(Yang et al, , 1997aYang, 1998;Chi et al, 2005a;Huang et al, 2006a), FISH mapping of centromeric sequences, telomeric sequences, cosmid clones and BAC clones (Scherthan,1990;Lin et al, 1991;Lee et al, 1993;Li et al, 2000a;Hartmann & Scherthan, 2004;Chi et al, 2005b;Huang et al, 2006b), and combined chromosome painting and satellite DNA mapping (Scherthan, 1995;Yang et al, 1997b,d;Huang et al, 2006c), provided direct evidence for the tandem fusion hypothesis. The chromosomal mechanism underlying the karyotype evolution in Muntiacus is well-established now: the karyotypes of all extant muntjacs have evolved from a common ancestor with a 2n = 70 acrocentric karyotype by extensive centromere-telomere fusions and several centric fusions.…”

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confidence: 99%

“…Some studies suggested that the repetitive DNA families at or near the centromeric and telomeric regions might facilitate illegitimate recombination between non-homologous chromosomes of muntjacs (Brinkley et al, 1984;Bogenberger et al, 1985Bogenberger et al, , 1987Benedum et al, 1986;Lin et al, 1991Lin et al, , 2004Lee et al, 1994Lee et al, , 1997Scherthan, 1995;Lee & Lin, 1996;Yang et al, 1997b;Li et al, 2000aLi et al, , b, 2002Hartmann & Scherthan, 2004). At present, four satellite DNA families are found in Muntiacus: satellite DNA families I, II, IV and V (Bogenberger et al, 1985;Lin et al, 1991Lin et al, , 2004Li et al, 2000bLi et al, , 2005.…”

mentioning

confidence: 99%

Cloning, Characterization, and FISH Mapping of Four Satellite DNAs from Black Muntjac (Muntiacus crinifrons)and Fea's Muntjac (M. feae)

Liu¹

2008

Zoological Research

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Abstract:Recent molecular cytogenetic studies demonstrate that extensive centromere-telomere fusions are the main chromosomal rearrangements underlying the karyotypic evolution of extant muntjacs. Although the molecular mechanism of tandem fusions remains unknown, satellite DNA is believed to have facilitated chromosome fusions by non-allelic homologous recombination. Previous studies detected non-random hybridization signals of cloned satellite DNA at the postulated fusion sites on the chromosomes in Indian and Chinese muntjacs. But the genomic distribution and organization of satellite DNAs in other muntjacs have not been investigated. In this study, we have isolated four satellite DNA clones (BMC5, BM700, BM1.1k and FM700) from the black muntjac (Muntiacus crinifrons) and Fea's muntjac (M. feae), and hybridized these four clones onto chromosomes of four muntjac species (M. reevesi, M. crinifrons, M. gongshanenisis and M. feae). Besides the predominant centromeric signals, non-random interstitial hybridization signals from satellite I and II DNA clones (BMC5, BM700 and FM700) were also observed on the arms of chromosomes of these four muntjacs. Our results provide additional support for the notion that the karyotypes of M. crinifrons, M. feae and M. gongshanensis have evolved from a 2n = 70 ancestral karyotype by a series of chromosome fusions.

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Characterization of ancestral chromosome fusion points in the Indian muntjac deer

Cited by 54 publications

References 33 publications

Comparison of the Chromosome Structures between the Chicken and Three Anserid Species, the Domestic Duck (<i>Anas platyrhynchos</i>), Muscovy Duck (<i>Cairina moschata</i>), and Chinese Goose (<i>Anser cygnoides</i>), and the Delineation of their Karyotype Evolution by Comparative Chromosome Mapping

Comparison of the Chromosome Structures between the Chicken and Three Anserid Species, the Domestic Duck (<i>Anas platyrhynchos</i>), Muscovy Duck (<i>Cairina moschata</i>), and Chinese Goose (<i>Anser cygnoides</i>), and the Delineation of their Karyotype Evolution by Comparative Chromosome Mapping

Chromatin Organization and Remodeling of Interstitial Telomeric Sites During Meiosis in the Mongolian Gerbil (Meriones unguiculatus)

Cloning, Characterization, and FISH Mapping of Four Satellite DNAs from Black Muntjac (<I>Muntiacus crinifrons</I>)and Fea's Muntjac (<I>M. feae</I>)

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