Characterization of anti-ganglioside antibodies present in normal human plasma

Mizutamari, R.K.; Wiegandt, Herbert; Nores, Gustavo A.

doi:10.1016/0165-5728(94)90048-5

Cited by 69 publications

(43 citation statements)

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“…Normal adult human serum contains anti-GM 1 antibodies of the IgM isotype (9). They are relatively low-affinity antibodies, cross-reacting with GA 1 and GD 1b and probably devoid of GM 1 -mediated biological activity (10).…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, very little information is available about how these autoantibodies are originated. Anti-GM 1 immunoglobulin M (IgM) antibodies are part of the antibody repertoire of normal humans (9) and similar antibodies but with higher affinity have been found in patients with neuropathy (6,10). Studies of anti-GM 1 IgM antibodies (13) cloned from neuropathy patients have shown that they are encoded by somatically mutated diverse V genes.…”

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confidence: 99%

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Normally Occurring Human Anti-GM₁Immunoglobulin M Antibodies and the Immune Response to Bacteria

et al. 2004

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Anti-GM 1 antibodies of the immunoglobulin M (IgM) isotype are normal components of the antibody repertoire of adult human serum. Using a sensitive high-performance thin-layer chromatography (HPTLC) immunostaining assay, we found that these antibodies were absent in the umbilical vein and children <1 month of age but could be detected after 1 month of age. Although most of the children older than 6 months of age were positive, there were still a few negative children. The appearance of anti-GM 1 IgM antibodies showed a perfect concordance with two well-characterized antibacterial antibodies, anti-Forssman and antiblood group A, which indicates a similar origin. We also studied IgM reactivity with lipopolysaccharides (LPSs) from gram-negative bacteria isolated from stool samples from healthy babies and from Escherichia coli HB101 in serum from individuals of different ages. We found a positive reaction with both LPSs in all the children more than 1 month of age analyzed, even in those that were negative for anti-GM 1 antibodies. Anti-GM 1 IgM antibodies were purified from adult serum by affinity chromatography and tested for the ability to bind LPSs from different bacteria. This highly specific preparation showed reactivity only with LPS from a strain of Campylobacter jejuni isolated from a patient with diarrhea. We conclude that normally occurring IgM antibodies are generated after birth, probably during the immune defense against specific bacterial strains.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Normally Occurring Human Anti-GM₁Immunoglobulin M Antibodies and the Immune Response to Bacteria

et al. 2004

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“…В работе использован модифицированный метод Mitzutamari и др. [10,11]. Вкратце, 1000 нг GD1a или GM1 ("Sigma", США) растворяли в 100 мл 100% метанола и с помощью пипетки заполняли лунки микротитрационного Работа посвящена поиску маркеров начальных стадий нейродегенеративных заболеваний у пожилых людей и предварительному исследованию распространенности этих заболеваний у пациентов данной возрастной группы.…”

Section: методикаunclassified

Serum igG antibodies to GD1a and GM1 gangliosides in elderly people

Kolyovska

2016

BIOMED KHIM

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Nowadays, the percentage of elderly people in society grows. Good nutrition and medical care help older people to have a normal life over 80 to 90 years. In the last ten years it is of critical importance to establish the clinical significance of serum IgG anti-GD1a and anti-GM1 ganglioside antibodies as potential biomarkers for neuronal damage in neurodegenerative diseases and immune-mediated neuropathies and demyelination. In the current study, the diagnostic values of IgG anti-GD1a and anti-GM1 antibodies were determined by the ELISA method in serum samples of 18 elderly patients (71-91 years). Significantly elevated serum IgG anti-GD1a and anti-GM1 antibodies titers were detected only in patients over 80 years. These data suggest that the immune-mediated neuropathies, neurodegeneration and demyelination in healthy elderly occur after 80 years old. Therefore, IgG anti-GD1a and anti-GM1 antibodies can serve as biomarkers, showing the nervous system dysfunction.

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“…Antibody binding to GM1 was quantified by enzyme-linked immunosorbent assay (ELISA) [7]. Titer values were calculated as the reciprocal of the serum dilution needed to obtain half-maximal antibody binding [16]. Rabbit sera were purified by GM1 affinity columns as described [7].…”

Section: Antibody Binding Assaysmentioning

confidence: 99%

Validation of a rabbit model of neuropathy induced by immunization with gangliosides

Moyano

Comín

Lardone

et al. 2008

Journal of the Neurological Sciences

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Characterization of anti-ganglioside antibodies present in normal human plasma

Cited by 69 publications

References 13 publications

Normally Occurring Human Anti-GM₁Immunoglobulin M Antibodies and the Immune Response to Bacteria

Normally Occurring Human Anti-GM₁Immunoglobulin M Antibodies and the Immune Response to Bacteria

Serum igG antibodies to GD1a and GM1 gangliosides in elderly people

Validation of a rabbit model of neuropathy induced by immunization with gangliosides

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Characterization of anti-ganglioside antibodies present in normal human plasma

Cited by 69 publications

References 13 publications

Normally Occurring Human Anti-GM1Immunoglobulin M Antibodies and the Immune Response to Bacteria

Normally Occurring Human Anti-GM1Immunoglobulin M Antibodies and the Immune Response to Bacteria

Serum igG antibodies to GD1a and GM1 gangliosides in elderly people

Validation of a rabbit model of neuropathy induced by immunization with gangliosides

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Product

Resources

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Normally Occurring Human Anti-GM₁Immunoglobulin M Antibodies and the Immune Response to Bacteria

Normally Occurring Human Anti-GM₁Immunoglobulin M Antibodies and the Immune Response to Bacteria