Characterization of antibacterial activity and mechanisms of two linear derivatives of bactenecin

Liu, Fei; Wang, Haimei; Cao, Songsong; Jiang, Chenggang; Hou, Juncai

doi:10.1016/j.lwt.2019.02.073

Cited by 7 publications

(6 citation statements)

References 44 publications

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“…In addition, there was no special damage in RF treated C. sakazakii samples compared with those of HW treated ones. We can deduced that the two treatments might destroy the membranes in a way of local pore formation, leading to leakage of the cytoplasm and ultimately disintegrating the entire cells (Liu, Wang, Cao, Jiang, & Hou, 2019). This finding matched with the statement that the microbial inactivation mechanism in low-moisture foods is likely due to injured cell membrane, different from protein denaturation in foods with high a w (Ling et al, 2019).…”

Section: Microstructure Of C Sakazakiisupporting

confidence: 78%

Thermal inactivation of Cronobacter sakazakii ATCC 29544 in powdered infant formula milk using thermostatic radio frequency

et al. 2020

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Section: Microstructure Of C Sakazakiisupporting

confidence: 78%

Thermal inactivation of Cronobacter sakazakii ATCC 29544 in powdered infant formula milk using thermostatic radio frequency

et al. 2020

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“… In the case of bactenectin, which normally contains a disulfide bridge, a number of linear analogues have been prepared, including peptides G2, R2, and DP7, which were found to exhibit OM disruption and moderate synergy (Table ). − In the case of indolicidin, structure–activity relationship (SAR) studies have led to the discovery of the synergists Indopt 10 and CLS001 (Table ). CLS001 is particularly effective and displays synergy with both vancomycin and azithromycin against multiple Gram-negative pathogens. , Marketed under the name Omiganan, CLS001 is also much less hemolytic than indolicidin and is currently in clinical trials for the treatment of skin-related infections. ,, …”

Section: Peptide-based Potentiatorsmentioning

confidence: 99%

Synergy by Perturbing the Gram-Negative Outer Membrane: Opening the Door for Gram-Positive Specific Antibiotics

Wesseling

Martin

2022

ACS Infect. Dis.

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New approaches to target antibacterial agents toward Gram-negative bacteria are key, given the rise of antibiotic resistance. Since the discovery of polymyxin B nonapeptide as a potent Gram-negative outer membrane (OM)-permeabilizing synergist in the early 1980s, a vast amount of literature on such synergists has been published. This Review addresses a range of peptide-based and small organic compounds that disrupt the OM to elicit a synergistic effect with antibiotics that are otherwise inactive toward Gram-negative bacteria, with synergy defined as a fractional inhibitory concentration index (FICI) of <0.5. Another requirement for the inclusion of the synergists here covered is their potentiation of a specific set of clinically used antibiotics: erythromycin, rifampicin, novobiocin, or vancomycin. In addition, we have focused on those synergists with reported activity against Gram-negative members of the ESKAPE family of pathogens namely, Escherichia coli , Pseudomonas aeruginosa , Klebsiella pneumoniae , and/or Acinetobacter baumannii . In cases where the FICI values were not directly reported in the primary literature but could be calculated from the published data, we have done so, allowing for more direct comparison of potency with other synergists. We also address the hemolytic activity of the various OM-disrupting synergists reported in the literature, an effect that is often downplayed but is of key importance in assessing the selectivity of such compounds for Gram-negative bacteria.

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“…ONPG can be catalysed by β-galactosidase to produce o-nitrophenol, which is absorbed at 420 nm. 56 A difference in the absorbance value was observed when luteolin was added to the culture ( Figure 3A). Evidently, the absorbance value increased after luteolin treatment.…”

Section: Cell Membrane Damage Assessmentmentioning

confidence: 99%

<p>The Antibacterial Activity and Mechanism of Action of Luteolin Against <em>Trueperella pyogenes</em></p>

Guo¹,

Liu²,

Zhang³

et al. 2020

IDR

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Purpose: This research aimed to investigate the antibacterial activity and potential mechanism of luteolin against T. pyogenes. Materials and Methods: The broth microdilution method was used to determine the minimum inhibitory concentrations (MICs) of luteolin against various T. pyogenes strains. The potential mechanism of action of luteolin was elucidated through testing and analysing the luteolin-induced alterations of T. pyogenes in several aspects, including cell wall, cell membrane, protein expression, nucleic acid content, topoisomerase activity and energy metabolism. Results: The MIC values of luteolin against various T. pyogenes isolates and ATCC19411 were 78 µg/mL. The increased cell membrane permeability, destruction of cell wall integrity and TEM images after exposure to luteolin showed that the cell wall and membrane were damaged. The content of total protein and nucleic acid in T. pyogenes decreased significantly after treatment with luteolin (1/2 MIC) for 12, 24, and 36 h. Moreover, a hypochromic effect was observed in the absorption spectrum of luteolin when deoxyribonucleic acid (DNA) was added. In addition, after treatment with luteolin, a decrease in nicked or relaxed DNA content, which was catalysed by T. pyogenes-isolated DNA topoisomerase, was observed. In addition, the adenosine triphosphate (ATP) content in cells and the activity of succinate dehydrogenase (SDH) both decreased when T. pyogenes was exposed to different concentrations (1/4 MIC, 1/2 MIC, 1 MIC, 2 MIC) of luteolin for 1 h. Conclusion: Luteolin showed distinct antibacterial activity against T. pyogenes by multiple actions, which mainly include destroying the integrity of the cell wall and cell membrane, influencing the expression of proteins, inhibiting nucleic acid synthesis, and interfering with energy metabolism.

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Characterization of antibacterial activity and mechanisms of two linear derivatives of bactenecin

Cited by 7 publications

References 44 publications

Thermal inactivation of Cronobacter sakazakii ATCC 29544 in powdered infant formula milk using thermostatic radio frequency

Thermal inactivation of Cronobacter sakazakii ATCC 29544 in powdered infant formula milk using thermostatic radio frequency

Synergy by Perturbing the Gram-Negative Outer Membrane: Opening the Door for Gram-Positive Specific Antibiotics

<p>The Antibacterial Activity and Mechanism of Action of Luteolin Against <em>Trueperella pyogenes</em></p>

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