Characterization of antifungal metabolite produced by a new strain Pseudomonas aeruginosa PUPa3 that exhibits broad-spectrum antifungal activity and biofertilizing traits

Kumar, Rajesh; Ayyadurai, Niraikulam; Pandiaraja, Pandurangan; Reddy, A. Vijender; Venkateswarlu, Y.; Prakash, Om; Sakthivel, Natarajan

doi:10.1111/j.1365-2672.2004.02435.x

Cited by 202 publications

(136 citation statements)

References 59 publications

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“…Plant growth-promoting ability of these bacteria is mainly because of the production of IAA (Patten and Glick, 2002), siderophores (Schippers et al, 1987) and antibiotics (Colyer and Mount, 1984). Production of antibiotics such as phenazine-1-carboxylic acid (PCA), pyocyanin, 2-acetamidophenol, pyrrolnitrin, pyoluteorin, phenazine-1-carboxylic acid, 2,4-diacetylphloro-glucinol, viscosinamide and tensin in different species of pseudomonads has been reported (Sunish Kumar et al, 2005). Production of siderophores has also been linked to the disease suppressing ability of certain fluorescent Pseudomonas species (Loper and Buyer, 1991).…”

Section: Fluorescent Pseudomonadsmentioning

confidence: 99%

Plant growth promoting rhizobacteria and their potential for biocontrol of phytopathogens

Ramadan¹,

Abdel-Hafez²,

Hassan³

et al. 2016

Afr. J. Microbiol. Res.

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Growth promotion and disease control by rhizobacteria are complex interrelated processes that involve direct and indirect mechanisms. The mechanisms include synthesis of some metabolites (auxin, cytokinin and gibberellins), induction of 1-aminocyclopropane-1-carboxylate (ACC) deaminase, production of siderophore, antibiotics, hydrogen cyanide hydrogen cyanide (HCN) and volatile compounds. They also include mineral solubilization competition, and induction of systemic resistance. These bacteria are suitable as soil inoculants because they have the potential for rapid and aggressive colonization. This feature alone is characterised as a disease control mechanism, which prevents the invasion of detrimental soil microorganisms onto the root surface. Inoculant-based plant growthpromoting rhizobacteria (PGPR) is applied extensively on agricultural crops to improve plants' growth and simultaneously reduce chemical inputs like fertilizer and pesticide which can cause environmental degradation. The structure of the rhizobacterial community is affected by several factors including plant genotype and is determined by the amount and composition of root exudates. In addition, soil type and fertility are the contributing factors that shape the community. This form of communication can affect plants' growth, nutrient status and also susceptibility to stress and pathogens in the host plant. PGPR inoculants cause diverse beneficial interactions among plants, which leads to sustainable and environment-friendly agriculture. The application of rhizosphere soil of agricultural crops with desirable bacterial populations is considered promising in both laboratory and greenhouse experiment. Further, a clearer understanding of the way PGPRs promote plants' growth can lead to expanded exploitation of these 'biofertilizers' in order to reduce the potential negative environmental effects associated with food and fiber production.

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Section: Fluorescent Pseudomonadsmentioning

confidence: 99%

Plant growth promoting rhizobacteria and their potential for biocontrol of phytopathogens

Ramadan¹,

Abdel-Hafez²,

Hassan³

et al. 2016

Afr. J. Microbiol. Res.

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“…With the help of sterilized borer wells were made in the agar plates and culture filtrate of the producing organism was poured in the well and incubated for 24 hours, zone of inhibition was measured for the activity of antimicrobial compounds. [10] A negative control well was too made with 50μl of the dissolving solvent (DMSO). A positive control was made by placing antibiotic disc (Ciprofloxacin, Fluconazole) on agar plate.…”

Section: Screening Of Antibiotic Producing Bacteriamentioning

confidence: 99%

Antimicrobial Activity of Bacillus Sp. Jg: Isolation, Identification and Process Optimization for the Production of Antimicrobial Metabolite Against Human Pathogens

Jain¹

2017

WJPPS

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Antibiotic resistance in bacteria has become a serious problem. Therefore, the search for new antibiotics is an important endeavour and is very much needed. In the present investigation, a number of bacterial strains were isolated from different soil samples and were tested for antimicrobial metabolite production. 85 bacterial isolates were isolated and the best activity was exhibited by the bacterial isolate P13. Physiological, biochemical and 16S rDNA gene sequence analysis strongly suggested that the isolate P13 belonged to Bacillus sp. Effect of medium, incubation period, pH and temperature variations for the maximum production of antimicrobial metabolite were optimized for maximum antimicrobial metabolite production. The maximum production of antimicrobial metabolite was observed in WYH under pH 7.0 and incubation temperature of 35 o C. Nutritional and culture conditions for the production of antimicrobial metabolite by this organism under shake-flask conditions were also optimized. Dextrose and peptone were found to be the best carbon and nitrogen sources for the production of antimicrobial metabolite by Bacillus sp. JG. Chloroform was the best extraction solvent for the isolation of the antimicrobial metabolite. The minimum inhibitory concentration of the metabolite against the test pathogens ranged between 0.9 to 2mg/ml. It may be suggested from the present study that further research is needed for World Journal of Pharmacy and Pharmaceutical Sciencesdetermining the chemical structure of metabolite which is responsible for bio-efficacy and to determine the cytotoxicity before it is used for commercialization purposes.

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“…DNA was prepared in the same way as for sequencing. PCR amplification was performed according to Kumar et al 27 .…”

Section: Detection Of Anti-pythium Genes In P Stutzerimentioning

confidence: 99%

Detection of diketopiperazine and pyrrolnitrin, compounds with anti-Pythium insidiosum activity, in a Pseudomonas stutzeri environmental strain

Thongsri¹,

Aromdee²,

Yenjai³

et al. 2014

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Aims. Screening of bacterial flora for strains producing metabolites with inhibitory effects on the human pathogenic oomycete Pythium insidiosum. Separation and characterization of extracts from Pseudomonas stutzeri with anti-Pythium inhibitory activity. Search for genes with anti-Pythium effect within the genome of P. stutzeri. Methods. A total of 88 bacterial strains were isolated from water resources in northeastern Thailand. Two screening methods were used to establish their inhibitory effects on P. insidiosum. One strain, P. stutzeri ST1302 was randomly chosen, and the extract with anti-P. insidiosum activity was fractionated and subfractionated using liquid column chromatography and purified by thin layer chromatography. The chemical structure of purified fractions was determined by Fourier transform infrared spectroscopy, nuclear magnetic resonance and mass spectrometry. Further, search for genes involved in the anti-Pythium activity (phenazine-1-carboxylic acid, 2,4-diacetylphloroglucinol, pyoluteorin and pyrrolnitrin) was undertaken in this P. stutzeri strain using primers described in the literature. Results. Anti-P. insidiosum activity was detected in 16 isolates (18.2%). In P. stutzeri ST1302, a subfraction labeled PYK7 exhibited strong activity against this oomycete. It was assigned to the diketopiperazines as cyclo(D-Pro-L-Val). In the search for genes, one gene region was successfully amplified. This corresponded to pyrrolnitrin. The results suggest the possibility of using the related metabolites against P. insidiosum. This is the first report on the inhibitory effects of P. stutzeri against this oomycete. The results may contribute to the development of antimicrobial drugs/probiotics against pythiosis.

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Characterization of antifungal metabolite produced by a new strain Pseudomonas aeruginosa PUPa3 that exhibits broad-spectrum antifungal activity and biofertilizing traits

Cited by 202 publications

References 59 publications

Plant growth promoting rhizobacteria and their potential for biocontrol of phytopathogens

Plant growth promoting rhizobacteria and their potential for biocontrol of phytopathogens

Antimicrobial Activity of Bacillus Sp. Jg: Isolation, Identification and Process Optimization for the Production of Antimicrobial Metabolite Against Human Pathogens

Detection of diketopiperazine and pyrrolnitrin, compounds with anti-Pythium insidiosum activity, in a Pseudomonas stutzeri environmental strain

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