Characterization of Aspartic Proteinase from Basidiomycete, Laetiporus sulphureus

Kobayashi, Hideyuki; Kim, Hyonok

doi:10.3136/fstr.9.30

Cited by 15 publications

(11 citation statements)

References 33 publications

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“…The enzyme called polyporopepsin (EC 3.4.23.29) was cloned and sequenced (Kobayashi et al 1989). Its N-terminal amino acid sequence bears close resemblance to that of aspartic proteinase from the basidiomycete Laetiporus sulphureus (Kobayashi and Kim 2003).…”

Section: Hydrolasesmentioning

confidence: 99%

Irpex lacteus, a white-rot fungus with biotechnological potential — review

et al. 2009

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White-rot fungi that are efficient lignin degraders responsible for its turnover in nature have appeared twice in the center of biotechnological research - first, when the lignin degradation process started being systematically investigated and major enzyme activities and mechanisms involved were described, and second, when the huge remediation potential of these organisms was established. Originally, Phanerochaete chrysosporium became a model organism, characterized by a secondary metabolism regulatory pattern triggered by nutrient (mostly nitrogen) limitation. Last decade brought evidence of more varied regulatory patterns in white-rot fungi when ligninolytic enzymes were also abundantly synthesized under conditions of nitrogen sufficiency. Gradually, research was focused on other species, among them Irpex lacteus showing a remarkable pollutant toxicity resistance and biodegradation efficiency. Systematic research has built up knowledge of biochemistry and biotechnological applicability of this fungus, stressing the need to critically summarize and estimate these scattered data. The review attempts to evaluate the information on I. lacteus focusing on various enzyme activities and bioremediation of organopollutants in water and soil environments, with the aim of mediating this knowledge to a broader microbiological audience.

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Section: Hydrolasesmentioning

confidence: 99%

Irpex lacteus, a white-rot fungus with biotechnological potential — review

et al. 2009

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“…Isolation, Purification, and Properties of EPS-2-1 According to the phenol-sulfuric acid method, the EPS purified from L. sulphureus contained 98.9% of carbohydrates and no absorbance was observed under the UV 280nm spectrophotometer, indicating that it was not contaminated with other substances including proteins [16]. The EPS was purified by a DEAE cellulose column with gradient elution using 0-0.3 M NaCl solutions.…”

Section: Resultsmentioning

confidence: 99%

Biochemical Characterization of the Exopolysaccharide Purified from Laetiporus sulphureus Mycelia

Seo

Kang²,

Park³

et al. 2011

J. Microbiol. Biotechnol.

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The extracellular polysaccharide (EPS) was isolated from mycelial cultures of Laetiporus sulphureus var. miniatus and purified by DEAE cellulose and Sephadex G-50 column chromatography. The purified EPS (EPS-2-1) was composed of only glucose units and its molecular mass was 6.95 kDa. The chemical structure of EPS-2-1 consisted of a main chain containing (1→4)-Glcp units with branches at the C-6 position of the chain carrying-Glcp-(1→4)-linked residues. The effect of purified EPS on immunomodulatory genes and proteins of the Bcl-2 family was observed using cultured U937 human leukemia cells. Of note, the levels of Bax and Bad proteins treated with the EPS (4 mg/ml) were approximately 23-and 18-times higher than those in non-treated cells, respectively. These results may suggest that the EPS purified from the mushroom L. sulphureus is associated with the activation of immunomodulatory mediators, Bax and Bad proteins.

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“…No absorbance was estimated at 280 nm in the UV spectrophotometer. This indicated that the exopolysaccharide was not contaminated with other substances, including proteins (Kobayashi and Kim, 2003). A linkage analysis was performed with full methylation, reduction, and acetylation of purified polysaccharide.…”

Section: Exopolysaccharide Productivity (G/l)mentioning

confidence: 99%

Initial acidic pH is critical for mycelial cultures and functional exopolysaccharide production of an edible mushroom, Laetiporus sulphureus var. miniatus JM 27

et al. 2010

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We conducted a time course experiment on mycelial cultures of Laetiporus sulphureus var. miniatus. The strain showed significant survival in an initial pH range of 2.0 to 7.0 for 24 days, during which time oxalic acid was accumulated. A structural analysis of purified exopolysaccharide suggested that it contained 96.1% glucose, and the mode of linkage was mainly → 4-Glcp-(1 → units, with branches at the C-6 position consisting of a Glcp-(1 → 4) linked side chain. An exopolysaccharide purified from the acidophilic strain was added to cultured U937 cells, resulting in significantly increased transcription levels of p53 and p21 genes.

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Characterization of Aspartic Proteinase from Basidiomycete, Laetiporus sulphureus

Cited by 15 publications

References 33 publications

Irpex lacteus, a white-rot fungus with biotechnological potential — review

Irpex lacteus, a white-rot fungus with biotechnological potential — review

Biochemical Characterization of the Exopolysaccharide Purified from Laetiporus sulphureus Mycelia

Initial acidic pH is critical for mycelial cultures and functional exopolysaccharide production of an edible mushroom, Laetiporus sulphureus var. miniatus JM 27

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