Characterization of Aspergillus flavus strains from Brazilian Brazil nuts and cashew by RAPD and ribosomal DNA analysis

Midorikawa, G. E. O.; Pinheiro, M; Vidigal, Bruna; Arruda, M.C.C. de; Costa, Fernando Ferreira; Παππάς, Γεώργιος; Ribeiro, S. G.; Freire, F. das C. O.; Miller, Robert G.

doi:10.1111/j.1472-765x.2008.02377.x

Cited by 27 publications

(19 citation statements)

References 24 publications

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“…In our study, plate washings were diluted to 10 -2 , resulting in positive amplification for all symptomatic samples. False negatives can be due to the presence of inhibitors or excess of target DNA, in this case using PCR internal controls should be highly desirable, as described by MIDORIKAWA et al (2008), to ensure the test's reliability.…”

Section: Resultsmentioning

confidence: 99%

Molecular detection of Erwinia psidii in guava plants under greenhouse and field conditions

et al. 2016

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Section: Resultsmentioning

confidence: 99%

Molecular detection of Erwinia psidii in guava plants under greenhouse and field conditions

et al. 2016

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“…Selain itu, teknik RAPD telah digunakan dalam beberapa penelitian analisis genetik mikroorganisme, di antaranya keragaman genetik Staphylococcus aureus (Morandi et al, 2010). Aplikasi RAPD dalam analisis keragaman genetik dan identifikasi cendawan telah banyak dilakukan seperti pada Phytophthora infestans (Atheya et al, 2005), Aspergillus flavus (Midorikawa et al, 2008) dan Colletotrichum truncatum (Sant'Anna et al, 2010). Saat ini penggunaan teknik molekuler untuk penetapan keragaman genetik Ganoderma sp.…”

Section: Pendahuluanunclassified

Analisis keragaman genetik Ganoderma spp. yang berasosiasi dengan tanaman kakao dan tanaman pelindungnya menggunakan Random Amplified Polymorphic DNA (RAPD) Genetic diversity analysis of Ganoderma spp. associated with cocoa and its shade trees using Random Amplified Polymorphic DNA (RAPD)

MINARSIH¹,

LINGGA²,

DARMONO³

et al. 2016

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Information on genetic diversity of Ganoderma spp.causing root rot disease in crops is important to developa proper strategy for the control of Ganoderma disease. Theobjectives of this research were to study the genetic diversityof Ganoderma spp. associated with cacao and its shade trees(Albazia faltacaria, Swietenia mahogani, Adenatheramicrosperma and Leucaena leucocephala) by randomamplified polymorphic DNA (RAPD) analysis. Fourty fivesamples of Ganoderma spp. were used in this research. Theresults showed that DNA amplification using 10 arbitraryoligonucleotide primers produced 220 DNA fragmentsshowing polymorphisms. The cluster analysis showed that 45number of Ganoderma samples had a high variability with thecoefficient value ranged from 0.71 to 0.91. Further analysisusing Winboot software showed that three groups ofGanoderma spp. had a high degree of confidence (>50 %),which were Ganoderma samples from sengon (Paraserianthessp.) of Tasikmalaya, sengon (Paraserianthes sp.) ofPalembang, and mahogany of Jember; whereas the othergroups of samples had a low degree of confidence (<50%).AbstrakInformasi tentang keragaman genetik Ganoderma spp.sebagai penyebab penyakit busuk akar pada tanamanperkebunan sangat diperlukan untuk menerapkan strategiyang tepat dalam upaya perlindungan tanaman perkebunan.Penelitian ini bertujuan untuk mengetahui keragaman genetikGanoderma spp. yang berasosiasi dengan tanaman kakao dantanaman pelindungnya (sengon, mahoni, saga dan lamtoro)dari berbagai wilayah di Indonesia menggunakan penandamolekuler random amplified polymorphic DNA (RAPD).Sebanyak 45 sampel Ganoderma spp digunakan dalampenelitian ini. Amplifikasi DNA dengan 10 primer terpilihmenghasilkan 220 fragmen DNA yang menunjukkan adanyapolimorfisme. Hasil analisis menunjukkan adanya keragamanyang cukup tinggi di antara sampel Ganoderma spp. daripohon inang dan wilayah yang berbeda, dengan nilaikoefisien 0,71-0,91. Berdasarkan analisis bootstrapdiketahui bahwa tiga kelompok sampel Ganoderma spp.memiliki tingkat kepercayaan yang tinggi (>50 %) yaitukelompok Ganoderma spp. yang berasosiasi dengan pohonsengon asal Tasikmalaya, sengon Palembang, dan mahoniJember; sedangkan pengelompokan lainnya menunjukkmenunjukkan tingkat kepercayaan yang rendah (<50 %).

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“…In some years, F. graminearum and A. flavus primers were also used. Primers used for F. graminearum were described for selectivity by Wang et al (2008), and primers for A. flavus were described for selectivity by Midorikawa et al 2008.…”

Section: Samplingmentioning

confidence: 99%

Field incidence of mycotoxins in commercial popcorn and potential environmental influences

Dowd

Johnson

2009

Mycotox Res

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Popcorn ear damage by insects and mycotoxin levels in kernels were monitored in several commercial popcorn fields in central Illinois over a 4-year period. Aflatoxin was rare, but fumonisin and deoxynivalenol (DON) were commonly encountered each year, and occurred at mean levels in fields up to 1.7 mg/kg (sample max. 2.77 mg/kg) and 1.9 mg/kg (sample max. 2.66 mg/kg), respectively. Neither fumonisin nor DON levels were significantly correlated with the percent of ears with visibly moldy insect-damaged kernels. Significant correlations were noted for the percent of ears with early caterpillar damage and both fumonisin and DON levels overall for some years and at specific sites in other years. Fumonisin levels were generally more highly correlated with insect damage than DON levels. Insect damaged kernels had 100- to 500-fold or greater levels of fumonisin compared to noninsect-damaged kernels, while DON levels were closer to 10- to 30-fold higher in insect damaged versus nondamaged kernels. A high percentage of DON-contaminated kernels were not insect damaged in 2007 and 2008. In some cases, differing mycotoxin levels for the same hybrid and same year planted at different locations appeared to be due to the prior crop. Higher DON levels in 2008 than other years were most likely associated with higher levels of rainfall and cooler temperatures than average during ear fill. While kernel sorters are reported to remove mycotoxin-contaminated popcorn kernels to acceptible levels, consideration of environmental factors that promote mycotoxins in popcorn should result in more effective control measures in the field.

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Characterization of Aspergillus flavus strains from Brazilian Brazil nuts and cashew by RAPD and ribosomal DNA analysis

Cited by 27 publications

References 24 publications

Molecular detection of Erwinia psidii in guava plants under greenhouse and field conditions

Molecular detection of Erwinia psidii in guava plants under greenhouse and field conditions

Analisis keragaman genetik Ganoderma spp. yang berasosiasi dengan tanaman kakao dan tanaman pelindungnya menggunakan Random Amplified Polymorphic DNA (RAPD) Genetic diversity analysis of Ganoderma spp. associated with cocoa and its shade trees using Random Amplified Polymorphic DNA (RAPD)

Field incidence of mycotoxins in commercial popcorn and potential environmental influences

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