Characterization of Atypical Aeromonas salmonicida Different Methods

Austin, Brian; Austin, Dawn A.; Dalsgaard, Inger; Guðmundsdóttir, Bjarnheiður K.; Høie, S.; Thornton, Jacinta; Larsen, J. L.; O'Hici, B.; Powell, Richard

doi:10.1016/s0723-2020(98)80008-8

Cited by 88 publications

(96 citation statements)

References 40 publications

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“…sahonicida is genetically homogenic throughout the world, and plasmid profiling is of little value in identifying the source of infection during outbreaks of furunculosis in salrnonids (Ssrum et al 1993). The present study shows, however, that the plasmid profiles vary between isolates of atypical A. salmonicjda, an observation also shown by Bast et al (1988), Pedersen et al (1996), Hanninen & Hirvela-Koski (1997), and Austin et al (1998). The majority of atypical A. salmonicida strains (95) could be divided into 7 different groups (I to VII) based on similarity in plasmid profiles.…”

Section: Discussionsupporting

confidence: 74%

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Grouping by plasmid profiles of atypical Aeromonas salmonicida isolated from fish, with special reference to salmonid fish

Sørum

Holstad²,

Lunder³

et al. 2000

Dis. Aquat. Org.

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Plasmid profile analyses were performed for 113 strains of atypical Aeromonas salmonicida and the reference strain A. salmonicida subsp. salmonicida ATCC 14174. The atypical A. salmonicida strains comprised 98 strains obtained from fish originating from 54 farms and 2 lakes in Norway, 10 strains from Canada ( 2 ) , Denmark (2), Finland ( l ) , Iceland (1) and Sweden (4), the reference strains NCMB 1109 and ATCC 15711 (Haemophilus piscium) of A. salmonicida subsp. achromogenes, and the type cultures A. salmonicida subsp. achrornogenes NCMB 11 10, A. salmonicida subsp. rnasoucida ATCC 27013 and A. salmonicida subsp, smithia CCM 4103. A total of 95 strains of atypical A. salmonicida were separated into 7 groups (I to VII) based on the plasmid profiles. Eighteen strains of atypical A, salmonicida had no common plasmid profile. The type strain NCMB 11 10 and the reference strain NCMB 1109 were included in group IV, and the type strain ATCC 27013 in group V, but the other reference and type strains had plasmid profiles different from all the other strains. An epidemiological link was documented between strains collected from different farms/localities in each of groups I, 111, V and VII. Physiological and biochemical characterizations were performed for 93 of the strains to investigate phenotypic differences between the plasmid groups. Group V11 strains and 3 strains with no common plasmid profile differed from the other groups in being catalase-negative. Differences in phenotypic characteristics were shown between the plasmid groups. However, significant variations in reactions for several phenotypic characteristics also occurred within each of the groups I to VII. The present study indicates that plasmid profiling may give useful epidemiological information during outbreaks of atypical A. salmonicida infections in fish. Additional comprehensive phenotypic characterisation is of limited value since the phenotypic characteristics in each plasmid group are not uniform.

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Section: Discussionsupporting

confidence: 74%

“…Austin et al (1998) found that isolates recovered from a -.vide range nf h~! s 2nd geographical locations were heterogeneous.…”

Section: Introductionmentioning

confidence: 99%

Grouping by plasmid profiles of atypical Aeromonas salmonicida isolated from fish, with special reference to salmonid fish

Sørum

Holstad²,

Lunder³

et al. 2000

Dis. Aquat. Org.

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show abstract

“…salmonicida. However, the isolates with brown-diffusible pigment obtained from loch sediment, with profiles of '5432004' and '5570544' (these became '5430004' 2 months later), could not be identified with the API 20NE Profile Index, although such variation in the results with API 20NE has been noted previously for A. salmonicida, with many different profiles accommodating bona fide representatives of the taxon (Austin and Austin 1993;Austin et al 1998). Furthermore, the sediment isolates of A. salmonicida were not pathogenic to rainbow trout.…”

Section: Resultsmentioning

confidence: 75%

“…salmonicida (American Type Culture Collection 14174, Rockville, MD, USA). The Aeromonas isolates were examined for the phenotypic traits described by Austin et al ( , 1998 and compared with the characteristics in Popoff (1984). All cultures were examined by the API 20NE rapid identification system (bioMérieux, Basingstoke, UK), with incubation at 25°C for 48 h. Whole cell agglutination of the sediment isolates of A. salmonicida was carried out with monospecific antiserum to A. salmonicida (titre 1:−8192; Austin et al 1998) produced in a New Zealand white rabbit (Schill et al 1989).…”

Section: Identification Of Bacterial Culturesmentioning

confidence: 99%

Isolation of Aeromonas salmonicida in association with purple-pigmented bacteria in sediment from a Scottish loch

Austin

Robertson

Wallace

et al. 1998

Lett Appl Microbiol

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“…It was found that SpaC, PknG and NanH presented better vaccine potential than SodC. Aeromonas hydrophila (A. hydrophila) causes aeromoniasis in fish [171]. Rauta et al [172] analyzed two antigenic outer membrane protein (Aha1) peptides as DNA vaccine candidates including them in a nanoparticle-based delivery system.…”

Section: Leptospira Interrogans (L Interrogans) Causes Leptospirosismentioning

confidence: 99%

Immunogenicity Prediction by VaxiJen: A Ten Year Overview

Zaharieva¹,

Димитров²,

Flower³

et al. 2017

J Proteomics Bioinform

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Characterization of Atypical Aeromonas salmonicida Different Methods

Cited by 88 publications

References 40 publications

Grouping by plasmid profiles of atypical Aeromonas salmonicida isolated from fish, with special reference to salmonid fish

Grouping by plasmid profiles of atypical Aeromonas salmonicida isolated from fish, with special reference to salmonid fish

Isolation of Aeromonas salmonicida in association with purple-pigmented bacteria in sediment from a Scottish loch

Immunogenicity Prediction by VaxiJen: A Ten Year Overview

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