Characterization of Auricularia auricula polysaccharides and its antioxidant properties in fresh and pickled product

Khaskheli, Shahzor Gul; Zheng, Wen-Bin; Sheikh, Saghir Ahmed; Khaskheli, Ashfaque Ahmed; Liu, Ying; Soomro, Aijaz Hussain; Feng, Xi; Sauer, Mary Ball; Wang, Yan Feng; Huang, Wen

doi:10.1016/j.ijbiomac.2015.08.020

Cited by 92 publications

(36 citation statements)

References 34 publications

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“…It has potential anti-tumour, anti-inflammatory, hypoglycemic, hypolipidemic and anticoagulant characteristics [2][3][4][5][6]. Furthermore, its extracts have been reported to possess antioxidant and radical scavenging properties [7][8][9]. Despite these important properties of A. auricula, the cultivation of this black fungus has been poorly explored, especially the degradation mechanism of cellulose or hemicellulose at the molecular level.…”

Section: Introductionmentioning

confidence: 99%

“…Among the various a-L-arabinofuranosidases, GH family-51 a-L-arabinofuranosidases have been well studied at the structural level. Analysis of these structures has revealed that GH family-51 a-L-arabinofuranosidases are composed of a catalytic domain characterized by a (b/a) 8 barrel with two conserved glutamate residues in the catalytic site [19].…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of a glycoside hydrolase family-51 α-L-arabinofuranosidase from Auricularia auricula

Zou

Sun

Yang

et al. 2017

Biotechnology & Biotechnological Equipment

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The cDNA encoding a-L-arabinofuranosidase was cloned from the edible fungus Auricularia auricula for the first time. The open reading frame of the a-L-arabinofuranosidase gene abf was 1953 bp encoding 650 amino acids, with a predicted protein molecular weight of 71.19 kDa and a theoretical isoelectric point of 5.23. The putative protein was predicted to belong to the glycoside hydrolase family-51. In addition, abf was cloned into the pET-32a vector and then expressed in Escherichia coli BL21. The recombinant protein, with an expected molecular weight, was observed in sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Moreover, the transcription levels of abf in response to different carbon sources were investigated in this study. The results showed that the expression of abf was mostly up-regulated when the mycelia were grown in different carbon sources, and L-arabinose or maltose induction had a significant effect on the expression of abf, which was 5.13-and 4.58-fold higher than that in the untreated control sample, respectively. In addition, the highest transcript levels induced by glucose and sucrose appeared on the third day and the levels were 2.47-and 3.11-fold higher compared to the control. These results laid a foundation for further studies on the a-L-arabinofuranosidase from A. auricula.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular characterization of a glycoside hydrolase family-51 α-L-arabinofuranosidase from Auricularia auricula

Zou

Sun

Yang

et al. 2017

Biotechnology & Biotechnological Equipment

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“…Up to today, it has become the fourth most important cultivated mushroom in the world. It has some pharmaceutical effects such as inhibiting lipid peroxidation, decreasing liver damage, scavenging radicals, and chelating metal ions in vitro and lowering blood lipids (Khaskheli et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…More and more attentions have been paid to the bioactive polysaccharides originated from natural sources. These polysaccharides not only serve as a free radical scavenger in the prevention of oxidative damage (Khaskheli et al, ), but also act as a strong antioxidant (Zhang, Wang, Zhang, & Wang, ). Besides these, they have other biological activities such as anti‐tumor, anti‐virus, anti‐aging, enhancing immunity, lowering blood glucose, and stimulating hematogenesis (Nguyen, Chen, et al, ; Thetsrimuang, Khammuang, Chiablaem, Srisomsap, & Sarnthima, ; Zhang, Li, Xia, & Lin, ; Zhu et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…In the past, the experimental material of A. auricula was ground into some certain particle diameter sizes ranging from 250 to 850 μm (Fan, Zhang, Yu, & Ma, ; Luo et al, ; Khaskheli et al, ; Zhang, Wang, Zhang, & Wang, ), which belongs to the routine grinding method. As one kind of new technology, superfine grinding is a useful tool for making superfine powder, referring to small solid particles with the size from 1 nm to 100 μm (Zhang et al, ).…”

Section: Introductionmentioning

confidence: 99%

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PEG‐based ultrasound‐assisted extraction of polysaccharides from superfine ground Auricularia auricular

Zhang

Wang

2017

J Food Process Preserv

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In this study, polyethylene glycol was used for the ultrasound‐assisted extraction of polysaccharides from superfine ground Auricularia auricula. The experimental factors including PEG molecule weight, PEG concentration, sample size, liquid–solid ratio, extraction temperature, and extraction time were optimized by the Box‐Behnken Design. The results showed that the optimal extraction conditions were a liquid–solid ratio of 39.27 ml/g, an extraction temperature of 91.94 °C and an extraction time of 32.44 min. Under these extraction conditions, the extraction yield of AAP was proved to be 25.75 ± 0.59%. The optimal extraction yield was well in close agreement with the value predicted by the regression model. Practical applications The PEG‐based ultrasound‐assisted extraction of polysaccharides from Auricularia auricular not only effectively decreases the extraction time, but also increases the extraction yield. Furthermore, the superfine grinding technology can greatly improve reactive surface to the largest content, thus enhancing the extraction yield.

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Purification and Structural Characterization of Dendrobium officinale Polysaccharides and Its Activities

Wang

et al. 2021

Chemistry & Biodiversity

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In this study, Dendrobium officinale polysaccharide (named DOPS‐1) was isolated from the stems of Dendrobium officinale by hot‐water extraction and purified by using Sephadex G‐150 column chromatography. The structural characterization, antioxidant and cytotoxic activity were carried out. Based on the results of HPLC, GC, Congo red experiment, together with periodate oxidation, Smith degradation, SEM, FT‐IR, and NMR spectral analysis, it expressed that DOPS‐1 was largely composed of mannose, glucose and galacturonic acid in a molar ratio of 3.2 : 1.3 : 1. The molecular weight of DOPS‐1 was 1530 kDa and the main chain was composed of (1→4)‐β‐D‐Glcp, (1→4)‐β‐D‐Manp and 2‐O‐acetyl‐(1→4)‐β‐D‐Manp. The measurement results of antioxidant activity showed that DOPS‐1 had the strong scavenging activities on hydroxyl radicals, DPPH radicals and superoxide radicals and the high reducing ability in vitro. Moreover, DOPS‐1 was cytotoxic to all three human cancer cells of MDA‐MB‐231, A549 and HepG2.

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Characterization of Auricularia auricula polysaccharides and its antioxidant properties in fresh and pickled product

Cited by 92 publications

References 34 publications

Molecular characterization of a glycoside hydrolase family-51 α-L-arabinofuranosidase from Auricularia auricula

Molecular characterization of a glycoside hydrolase family-51 α-L-arabinofuranosidase from Auricularia auricula

PEG‐based ultrasound‐assisted extraction of polysaccharides from superfine ground Auricularia auricular

Purification and Structural Characterization of Dendrobium officinale Polysaccharides and Its Activities

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