Characterization of avian myeloblastosis-associated virus DNA intermediates

Bergmann, D G; Souza, Lawrence M.; Baluda, M A

doi:10.1128/jvi.34.2.366-372.1980

Cited by 15 publications

(14 citation statements)

References 22 publications

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“…The positions of hybridization probes are shown underneath the provirus. The restriction map has been compiled from Bergmann et al (1980), Souza and Baluda (1980) and the unpublished data of D.Westaway. Therefore the diagram represents a consensus MAV provirus.…”

Section: Resultsmentioning

confidence: 99%

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Identification of a provirally activated c-Ha-ras oncogene in an avian nephroblastoma via a novel procedure: cDNA cloning of a chimaeric viral-host transcript.

Westaway¹,

Papkoff²,

Moscovici³

et al. 1986

The EMBO Journal

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Retroviruses without oncogenes often exert their neoplastic potential as insertional mutagens of cellular proto-oncogenes.This may be associated with the production of chimaeric viral -host transcripts; in these cases, activated cellular genes can be identified by obtainig cDNA clones of bipartite RNAs. This approach was used in the analysis of chicken nephroblastomas induced by myeloblastosis-associated virus (MAV). One tumor contained a novel mRNA species initiated within a MAV LTR. cDNA cloning revealed that this mRNA encodes a protein of 189 amino acids, identical to that of normal human Ha-ras-1 at 185 positions, including positions implicated in oncogenic activation of ras proto-oncogenes; there are no differences between the coding sequences of presumably normal Ha-ras cDNA clones from chicken lymphoma RNA and the tumor-derived cDNAs. The chimaeric mRNA in the nephroblastoma is at least 25-fold more abundant than c-Ha-ras mRNA in normal kidney tissue, and a 21-kd ras-related protein is present in relatively lare amounts in the tumor. We conclude that a quantitative change in cHa-ras gene expression results from an upstream insertion mutation and presumably contributes to tumorigenesis in this single case. Little or no increase in c-Ha-ras RNA or protein was observed in other nephroblastomas.

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Section: Resultsmentioning

confidence: 99%

“…Therefore the diagram represents a consensus MAV provirus. The HindruI site 1.2 kb from the 3' end of the genome may not be present in all MAV isolates (Bergmann et al, 1980). The U5 probe has been described previously (Payne et al, 1981).…”

Section: Resultsmentioning

confidence: 99%

Identification of a provirally activated c-Ha-ras oncogene in an avian nephroblastoma via a novel procedure: cDNA cloning of a chimaeric viral-host transcript.

Westaway¹,

Papkoff²,

Moscovici³

et al. 1986

The EMBO Journal

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“…[a-32P]dCTP-labeled DNA was hybridized to DNA in Southern blots as described by Wahl et al (28), washed (5), and autoradiographed (20).…”

Section: (9)mentioning

confidence: 99%

Vertebrate DNAs contain nucleotide sequences related to the transforming gene of avian myeloblastosis virus

Bergmann¹,

Souza²,

Baluda³

1981

J Virol

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Avian myeloblastosis virus contains a continuous sequence of approximately 1,000 nucleotides which may represent a gene (amv) responsible for acute myeloblastic leukemia in chickens. This sequence appears to have been acquired from chicken DNA and to be substituted for the envelope gene in the viral genome. We used hybridization probes enriched for the amv sequences and conditions that facilitate annealing of partially homologous nucleotide sequences to show that cellular sequences related to amv are present in the genomes of all vertebrates ranging from amphibians to humans but were not detected in fish, sea urchins, or Escherichia coli. In contrast to the preceding findings, nontransforming endogenous proviral nucleotide sequences closely related to the remainder of the avian myeloblastosis virus genome and to the entire myeloblastosis-associated helper virus are present only in chicken DNA. The amv-related cellular sequences appear to be highly conserved during evolution and to be contained at only one or a few locations in the genome of vertebrates. Within closely related species, they appear to share common evolutionary genetic loci. These findings and similar ones obtained with other highly oncogenic retroviruses containing a transforming gene suggest a general mechanism for acquisition of viral oncogenic sequences and an essential role for these sequences in the normal cellular state.

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“…of EcoRI-cleaved DNA from uninfected or MAV-2-infected CEF. High-molecular-weight cellular DNA was digested with EcoRI, electrophoresed in 0.7%o agarose gels, blotted, and hybridized with 125I-labeled AMV-S RNA as described previously (4,15,16). Autoradiographs of Southern blots of KpnI-cleaved DNA from uninfected or MAV-2-infected CEF.…”

Section: Fig 3 Autoradiographs Of Southern Blotsmentioning

confidence: 99%

“…CEF cultures from a single 11-day-old C/E chicken embryo (Spafas, Inc.) negative for group-specific antigen and for chicken helper factor were infected with MAV-2 at a multiplicity of 1 to 5 infectious units per cell. High-molecular-weight (-3 x 107) cellular DNA from the MAV-2-infected CEF was extracted, precipitated by the method of Hirt, and purified as previously described (4,6,7,9).…”

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confidence: 99%

DNA of avian myeloblastosis-associated virus type 2 integrates at multiple sites in the chicken genome

Bergmann¹,

Baluda²

1980

J Virol

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The cellular sites of integration of the avian myeloblastosis-associated virus type 2 (MAV-2) DNA have been examined by Southern blot analysis of cellular DNA from infected cloned and uncloned chicken embryonic fibroblasts. Provirus-cell juncture fragments were not detected in restriction enzyme digests of DNA from MAV-2-infected uncloned cells. However, each MAV-2-infected cell clone examined produced a unique set of junctive bands. Thse findings indicate that multiple sites of integration exists for MAV-2 proviruses in cellular DNA.

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Characterization of avian myeloblastosis-associated virus DNA intermediates

Cited by 15 publications

References 22 publications

Identification of a provirally activated c-Ha-ras oncogene in an avian nephroblastoma via a novel procedure: cDNA cloning of a chimaeric viral-host transcript.

Identification of a provirally activated c-Ha-ras oncogene in an avian nephroblastoma via a novel procedure: cDNA cloning of a chimaeric viral-host transcript.

Vertebrate DNAs contain nucleotide sequences related to the transforming gene of avian myeloblastosis virus

DNA of avian myeloblastosis-associated virus type 2 integrates at multiple sites in the chicken genome

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