Characterization of bacterial diversity in two aerated lagoons of a wastewater treatment plant using PCR–DGGE analysis

Abstract

Aerated lagoons are commonly used for domestic and industrial wastewater treatment due to their low cost and minimal need of operational requirements. However, little information is known regarding microbial communities that inhabit these ecosystems. In this study, a 16S-DGGE approach was used to estimate bacterial diversity and to monitor community changes in two aerated lagoons from a wastewater treatment plant receiving urban and industrial effluents. Pronounced shifts between bacterial communities collected in winter-spring and summer-autumn months were detected. Temperature, dissolved oxygen (DO) and pH were the variables that most influenced the bacterial communities. Phylogenetic affiliation of predominant members was assessed by the determination of the 16S rDNA sequence of correspondent bands. Affiliations to Cytophaga-Flexibacter-Bacteroides (CFB) group, Firmicutes, and beta- and epsilon-proteobacteria were found.

Review of Molecular Techniques for the Identification of Bacterial Communities in Biological Effluent Treatment Facilities at Pulp and Paper Mills

“…It has been mentioned that this technique does not allow the quantification of cells. However, Moura et al (2009) examined DGGE patterns using two indexes, the Shannon-Weaver index and the equitability index. The diversity was calculated based on the relative intensity of DNA bands obtained from wastewater samples from cheese industry.…”

Evaluating sulphate removal and identifying the bacterial community present in acid mine drainage treated with synthetic domestic wastewater sludge

“…Sulphides are oxidised to elemental sulphur, which is deposited externally as sulphur globules that can be removed from the wastewater system (Brune, 1995;Pott and Dahl, 1998;Frigaard and Dahl, 2008). Due to their robustness and the nature of their sulphur metabolism, anoxygenic sulphur bacteria have been widely used in wastewater treatment processes as they enable a cost-effective, low-maintenance treatment of sulphide-rich wastewater (Malik et al, 2008;Mara, 2008;Moura et al, 2009).…”

Method optimization for denaturing gradient gel electrophoresis (DGGE) analysis of microflora from Eucalyptus sp. wood chips intended for pulping

“…Many of these studies relied on traditional culturing techniques to study microflora. Molecular methods relying on DNA or RNA extracted directly from environmental samples such as polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), automated ribosomal intergenic spacer analysis (ARISA) and terminal restriction fragment length polymorphism (T-RFLP) are increasingly being used, particularly in analyzing community structure of microorganisms from different environments, for example, soil (Gelsomino et al, 1999;Maarit-Niemi et al, 2001;Hoshino and Matsumoto, 2007;Xue et al, 2008), compost (Adams and Frostick, 2009;Novinscak et al, 2009), wastewater (Moura et al, 2009;Liu et al, 2010;Chen et al, 2013), food (Greaves, 1975;Miller et al, 1999;Ji et al, 2004;Handschur et al, 2005) and decaying chip piles intended for fuel (Rajala et al, 2010;Rajala et al, 2011). Several recent reports focused on understanding the microbial effects on storage of woody biomass intended for biofuel production, as well as composting (Ashraf et al, 2007;Adams and Frostick, 2009;Novinscak et al, 2009).…”