Characterization of bep1 and bep4 antigens involved in cell interactions during Paracentrotus lividus development

Romancino, Daniele P.; Ghersi, Giulio; Montana, Giovanna; Bonura, Angela; Perriera, Salvatore; Carlo, Marta Di

doi:10.1111/j.1432-0436.1992.tb00487.x

Cited by 27 publications

(14 citation statements)

References 20 publications

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“…In the latter, at early cleavage stages, inductive interactions between neighbouring cells are probably involved in such a mechanism. On the other hand, zygotic and maternal (Romancino et al, 1992;Di Carlo, personal communication) genes whose expression is spatially restricted to the animal region have been isolated. These genes encode for neither transcription factors nor growth factors but their protein products show also the same asymmetrical distribution in the embryo.…”

Section: Introductionmentioning

confidence: 99%

Expression of homeobox-containing genes in the sea urchin (Parancentrotus lividus) embryo

Bernardo

Russo²,

Oliveri³

et al. 1994

Genetica

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Two homeobox-containing genes that belong to different homeodomain classes have been isolated from a sea urchin genomic library. One, PlHbox11, is the sea urchin homologue of the human and mouse Hox B3 gene, the other, PlHbox12, shows about 55% identity with paired class genes. Expression profile analysis of the two sea urchin Hbox genes suggests that they play different roles during embryogenesis. In fact, PlHbox11 transcripts are rare and are detected only in the pluteus larva and in the Aristotle's lantern and intestine of the adult. The PlHbox12 gene is, on the contrary, transiently expressed in the very early embryo already at the four cell stage; it accumulates at the 64 cell stage and disappears at later stages of development. In situ hybridization experiments to 16 and 32 cell stage whole mount embryos showed localization of the PlHbox12 mRNA to part of the mesomere-macromere region of the early cleavage embryo. These observations suggest a possible role of this gene in early events of cell specification.

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Section: Introductionmentioning

confidence: 99%

Expression of homeobox-containing genes in the sea urchin (Parancentrotus lividus) embryo

Bernardo

Russo²,

Oliveri³

et al. 1994

Genetica

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“…Davidson (30,31) suggested that mi- cromeres are responsible for a cascade of cell interactionactivated events, leading to the early morphogenesis of the sea urchin embryo. That the bepl and bep4 proteins may have a role in cell interactions was suggested by experiments in which treatment of dissociated embryo cells with Fabs against bepl and bep4 proteins caused inhibition of cell reaggregation (13). On the other hand, these proteins may have other roles because, as previously shown (13), they are found not only on the cell surface but also within the cytoplasm.…”

Section: Discussionmentioning

confidence: 79%

“…We hypothesize that the territorial location of the two maternal mRNAs and their products described here in one-halfofthe unfertilized egg represents the asymmetry that starts such a cascade of gene activation. It is of potential interest that the proteins produced by these two maternally derived mRNAs appear to be associated with the cell surface (13).…”

Section: Discussionmentioning

confidence: 99%

“…They belong to a multigene family and code for proteins that become localized in the cell surface during early embryogenesis and may be relevant for cell adhesion (12,13). Their molecular mass is 33 kDa, and their messengers show a particular structural organization; two well-conserved domains surrounding a single specific domain (12,13). We report here that both these mRNAs and the corresponding proteins are located exclusively in one-half of the unfertilized egg (probably the animal half) and become localized at the animal pole immediately after fertilization.…”

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confidence: 99%

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Spatial distribution of two maternal messengers in Paracentrotus lividus during oogenesis and embryogenesis.

Carlo

Romancino

Montana

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

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We demonstrated that two mRNAs that are synthesized during the vitellogenic period of oogenesis and that code for cell surface proteins are asymmetrically distributed in the unfertilized egg of Paracentrotus lividus. At fertilization, these RNAs rapidly localize in the cortical zone at the animal pole of the egg. They are then detected in the mesomeres and the macromeres, but not in the micromeres, and thereafter are found in the ectoderm but not in the vegetal plate, mesenchyme cells, or early intestine. They disappear in late gastrula. The proteins synthesized by these mRNAs show the same territorial location during the period examined here, which included the unfertilized egg and the 16-blastomere stage. These conclusions were reached on the basis of in situ hybridization and immuntaning experiments, as well as Northern and Western blot analyses of isolated blastomeres. The possible sinificance of this asymmetric distribution of these two mRNAs and proteins in the establishment of the animal/vegetal axis is discussed.

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“…In sea urchins the localization process has not yet been studied because only recently three maternal mRNAs, called bep1 , bep3 and bep4 , asymmetrically distributed in P. lividus egg have been identified [9, 10]. These messengers encode for cell surface proteins that are involved in cell–cell interactions and may be relevant for the determination of the animal side of the sea urchin embryo [11, 12]. Moreover it has been demonstrated that cytoskeleton is involved in their localization and that the 3′UTRs of these mRNAs are able to bind to a 54‐kDa protein that we suggested to name LP54 (localizing protein) [14].…”

Section: Introductionmentioning

confidence: 99%

Folding and binding activity of the 3′UTRs of Paracentrotus lividus bep messengers

et al. 1998

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Bep mRNAs are localized at the animal pole of P. lividus eggs. In the present communication the secondary structures of the 3PUTRs of the bep1, bep3 and bep4 mRNAs are reported. The minimal lenghts of these regions required to bind the 54-kDa protein, previously shown to be involved in localization and anchoring of these RNAs, is estimated. Microinjection of the bep3 3PUTR into egg shows that this RNA fragment is also able to become localized to one of the egg poles, as happens for the entire bep3 RNA.z 1998 Federation of European Biochemical Societies.

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Characterization of bep1 and bep4 antigens involved in cell interactions during Paracentrotus lividus development

Cited by 27 publications

References 20 publications

Expression of homeobox-containing genes in the sea urchin (Parancentrotus lividus) embryo

Expression of homeobox-containing genes in the sea urchin (Parancentrotus lividus) embryo

Spatial distribution of two maternal messengers in Paracentrotus lividus during oogenesis and embryogenesis.

Folding and binding activity of the 3′UTRs of Paracentrotus lividus bep messengers

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