Characterization of beta-tricalcium phosphate as a novel immunomodulator

Tai, Sachiko; Cheng, Jinyan; Ishii, Hidee; Akimoto, Shingo; Satoh, Takatomo; Yamamoto, Kazumi; Nakajima, Takashi; Karaki, Sachiko; Suzuki, Eiji; Yamaguchi, Ken; Maruyama, Kouji

doi:10.1016/j.intimp.2013.12.024

Cited by 11 publications

(19 citation statements)

References 26 publications

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“…26,27 b-TCP also induced the protein level of macrophage inflammatory protein 1 alpha in J774A.1 cells. 26 Both proteins are known to stimulate recruitment of various immune cells to the site of inflammation, thus upregulation of these proteins in the presence of b-TCP triggers and enhances the migration of a huge number of immune cells towards the area surrounding b-TCP in vivo. 6,26 The recruitment of these immune cell types to the vicinity of b-TCP lead to degradation of b-TCP via phagocytosis.…”

Section: Histological Evaluationsmentioning

confidence: 88%

“…25 Upon implantation, the inflammatory reaction is the first stage of the bone healing whereby inflammatory cells (macrophages, monocytes, lymphocytes, and polymorphonuclear cells) are recruited and colonize the b-TCP surface. 6,25,26 Tai et al demonstrated that subcutaneous implantation of b-TCP in normal B6 mice evoked extensive migration of immune cells into the area surrounding the implantation site. The b-TCP was initially colonized by neutrophils, followed by lymphocytes, histiocytes (macrophages), and fibroblasts, and finally by multinucleated giant cells.…”

Section: Histological Evaluationsmentioning

confidence: 99%

“…The b-TCP was initially colonized by neutrophils, followed by lymphocytes, histiocytes (macrophages), and fibroblasts, and finally by multinucleated giant cells. 6,26 Studies have reported that human peripheral blood mononuclear cells and J774A.1 cells (monocyte/macrophage cells derived from mice) incubated with b-TCP exhibited upregulation of the protein level of tumor necrosis factor alpha. 26,27 b-TCP also induced the protein level of macrophage inflammatory protein 1 alpha in J774A.1 cells.…”

Section: Histological Evaluationsmentioning

confidence: 99%

“…26 Both proteins are known to stimulate recruitment of various immune cells to the site of inflammation, thus upregulation of these proteins in the presence of b-TCP triggers and enhances the migration of a huge number of immune cells towards the area surrounding b-TCP in vivo. 6,26 The recruitment of these immune cell types to the vicinity of b-TCP lead to degradation of b-TCP via phagocytosis. Eggli et al found a cluster of macrophages packed with granular b-TCP on the implantation site in a rabbit model, which suggests that macrophages may play an active role in intracellular degradation of small detached ceramic particles.…”

Section: Histological Evaluationsmentioning

confidence: 99%

“…Eggli et al found a cluster of macrophages packed with granular b-TCP on the implantation site in a rabbit model, which suggests that macrophages may play an active role in intracellular degradation of small detached ceramic particles. 28 Human peripheral blood mononuclear cells and J774A.1 cells were also found to incorporate b-TCP by phagocytosis, 26,27 and recent studies demonstrated the involvement of foreign body multinucleated giant cells (FBGCs) in degrading b-TCP. 29,30 FBGCs form by fusion of monocytes/ macrophages only under pathological conditions or at the surface of a foreign material such as b-TCP.…”

Section: Histological Evaluationsmentioning

confidence: 99%

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In vivo efficacy and toxicity of synthesized nano-β-tricalcium phosphate in a rabbit tibial defect model

Ooi

Kasim

Shaari

et al. 2018

Toxicology Research and Application

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Previous studies of the biocompatibility of b-tricalcium phosphate (b-TCP) focused on bulk-sized b-TCP, and little is known about the biocompatibility of nano b-TCP particles (nb-TCP). The objectives of this study were to synthesize nb-TCP particles and determine their efficacy in a rabbit tibial defect model. The nb-TCP particles were first synthesized using a wet chemical precipitation process. The particles were then implanted in the left tibia of New Zealand white rabbits, and the defect site healing was evaluated for a period of 16 weeks using radiography, computed tomography, and histology. Data were compared with those of a sham (empty) control. Results showed that the defect site treated with nb-TCP particles did not heal completely after 16 weeks, whereas full cortical bone recovery was observed in the sham control group of rabbits. Histopathological examination showed that the nb-TCP particles caused an excessive and prolonged inflammatory response by the host. The nano-scaled size and biodegradability of the synthesized nb-TCP particles may have been responsible for this progressive and extended inflammatory response, which delayed the bone healing process. The underlying mechanism for this effect remains unclear and warrants further investigation.

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Section: Histological Evaluationsmentioning

confidence: 88%

Section: Histological Evaluationsmentioning

confidence: 99%

Section: Histological Evaluationsmentioning

confidence: 99%

Section: Histological Evaluationsmentioning

confidence: 99%

Section: Histological Evaluationsmentioning

confidence: 99%

See 3 more Smart Citations

In vivo efficacy and toxicity of synthesized nano-β-tricalcium phosphate in a rabbit tibial defect model

Ooi

Kasim

Shaari

et al. 2018

Toxicology Research and Application

View full text Add to dashboard Cite

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Effects of beta-tricalcium phosphate particles on primary cultured murine dendritic cells and macrophages

Tai

Cheng

Ishii

et al. 2016

International Immunopharmacology

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Beta-tricalcium phosphate promotes osteogenic differentiation of bone marrow-derived mesenchymal stem cells through macrophages

et al. 2021

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Macrophages are vital regulators of skeletal remodeling and osseous repair. Beta-tricalcium phosphate (β-TCP) is a synthetic ceramic biomaterial that has shown promise as bone substitute. However, whether and how β-TCP affects osteogenesis-related responses of macrophages has rarely been studied. The aims of this study were to explore (a) the effects of β-TCP on osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) co-cultured with macrophages and (b) on macrophage polarization as well as macrophage gene and protein expression profiles. BMSC osteogenic differentiation capacity in vitro was enhanced in β-TCP-induced co-cultured BMSCs compared to that in BMSC monocultures. We also found that macrophages induced with 25 mg ml−1 β-TCP extract had more significant immune responses and switched to the M2 phenotype. Expression levels of the Wnt signaling pathway modulators wingless-type MMTV integration site family, member 6 (WNT6) and Wnt inhibitory factor 1 (WIF1) were upregulated and downregulated, respectively, in macrophages treated with β-TCP extract. Our findings suggest that β-TCP enhances osteogenic differentiation of BMSCs by inducing macrophage polarization and by regulating the Wnt signaling pathway, thereby highlighting its therapeutic potential for bone healing through osteoimmunomodulatory properties.

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Characterization of beta-tricalcium phosphate as a novel immunomodulator

Cited by 11 publications

References 26 publications

In vivo efficacy and toxicity of synthesized nano-β-tricalcium phosphate in a rabbit tibial defect model

In vivo efficacy and toxicity of synthesized nano-β-tricalcium phosphate in a rabbit tibial defect model

Effects of beta-tricalcium phosphate particles on primary cultured murine dendritic cells and macrophages

Beta-tricalcium phosphate promotes osteogenic differentiation of bone marrow-derived mesenchymal stem cells through macrophages

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